Spatial Variation and Environmental Parameters Affecting the Abundant and Rare Communities of Bacteria and Archaea in the Sediments of Tropical Urban Reservoirs

Pierangeli, Gabrielle Maria Fonseca; Domingues, Mércia Regina; Choueri, Rodrigo Brasil; Hanisch, Werner Siegfried; Gregoracci, Gustavo Bueno; Benassi, Roseli Frederigi

doi:10.1007/s00248-022-02047-z

Cited by 6 publications

(7 citation statements)

References 73 publications

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“…In contrast, DO, which often affects bacterial communities in aquatic ecosystems 72 , does not vary significantly over the course of the year, so it was not considered to affect bacterioplankton communities in Billings surface water. Consistent with our results on the water surface from Billings reservoir, Pierangeli et al 73 recently found that DO and groundwater pH showed no statistical differences among sampling sites. According to our results, the water was neutral, with a relatively large increase in pH in September, the month of transition from the dry winter to the hot rainy season.…”

Section: Discussionsupporting

confidence: 93%

Temporal patterns of bacterial communities in the Billings Reservoir system

Marcondes,

Pessôa,

José da Silva Duarte

et al. 2024

Sci Rep

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In this study, high-throughput sequencing of 16S rRNA amplicons and predictive PICRUSt functional profiles were used to perform a comprehensive analysis of the temporal bacterial distribution and metabolic functions of 19 bimonthly samples collected from July 2019 to January 2020 in the surface water of Billings Reservoir, São Paulo. The results revealed that most of the bacterial 16S rRNA gene sequences belonged to Cyanobacteria and Proteobacteria, which accounted for more than 58% of the total bacterial abundance. Species richness and evenness indices were highest in surface water from summer samples (January 2020), followed by winter (July 2019) and spring samples (September and November 2019). Results also showed that the highest concentrations of sulfate (SO4–2), phosphate (P), ammonia (NH3), and nitrate (NO3-) were detected in November 2019 and January 2020 compared with samples collected in July and September 2019 (P < 0.05). Principal component analysis suggests that physicochemical factors such as pH, DO, temperature, and NH3 are the most important environmental factors influencing spatial and temporal variations in the community structure of bacterioplankton. At the genus level, 18.3% and 9.9% of OTUs in the July and September 2019 samples, respectively, were assigned to Planktothrix, while 14.4% and 20% of OTUs in the November 2019 and January 2020 samples, respectively, were assigned to Microcystis. In addition, PICRUSt metabolic analysis revealed increasing enrichment of genes in surface water associated with multiple metabolic processes rather than a single regulatory mechanism. This is the first study to examine the temporal dynamics of bacterioplankton and its function in Billings Reservoir during the winter, spring, and summer seasons. The study provides comprehensive reference information on the effects of an artificial habitat on the bacterioplankton community that can be used to interpret the results of studies to evaluate and set appropriate treatment targets.

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Section: Discussionsupporting

confidence: 93%

Temporal patterns of bacterial communities in the Billings Reservoir system

Marcondes,

Pessôa,

José da Silva Duarte

et al. 2024

Sci Rep

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“…Surface sediment samples (0-10 cm) were collected from each station in triplicates using a grab sediment sampler (Van Veen, OSIL, Hants, UK). The sediment samples were screened with a 2 mm net to remove stones and plant roots and sealed in aseptic plastic bags [26]. Total organic carbon (TOC) in the sediment was determined using the potassium dichromate oxidation-reduction volumetric method, total nitrogen (TN) was determined via Kjeldahl titration, total phosphorus (TP) was determined using spectrophotometry, and sulfide was analyzed by methylene blue spectrophotometry.…”

Section: Sample Collection and Analysismentioning

confidence: 99%

Dissolved Inorganic Nutrient Biogeochemistry in an Urbanized Coastal Region: A Study of Dapeng Cove, Shenzhen

Tong,

Chen,

Zhang

2023

Sustainability

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Dissolved inorganic nutrients are pivotal in maintaining the material and energy balance of marine ecosystems, impacting the survival and dynamic succession of marine organisms. To gain a deeper understanding of the source and sink characteristics of dissolved inorganic nutrients in bays affected by human activities and to elucidate the processes involving filter-feeding shellfish in relation to these nutrients, this study investigated the source and sink dynamics of dissolved inorganic nutrients in the Dapeng Cove sea area of Shenzhen. Over the past decade, a significant change in the N/P ratio within the survey area has been observed, suggesting a shift in nutrient limitation from nitrogen to phosphorus or phosphorus–silicon limitation. This induced change in the N/P ratio, along with Si/N and Si/P ratios, may facilitate the growth of cyanobacteria and, subsequently, alter the proportions of diatoms, dinoflagellates, and cyanobacteria. Seasonal fluctuations in human disturbance intensity and precipitation determine the seasonal and spatial distribution of nutrients in the bay, thereby influencing the bay ecosystem metabolism. The Land–Ocean Interactions in the Coastal Zone (LOICZ) model analysis revealed that the bay represents a major source of inorganic nitrogen and a source of phosphate in spring, summer, and autumn, while acting as a sink for phosphate in winter. Furthermore, rivers and groundwater represent the primary sources of phosphate and inorganic nitrogen in the bay. The bay exhibits an annual net ecosystem metabolic rate of 7.06 mmol C/m2/d, with denitrification dominating the nitrogen cycle at 12.65 mmol C/m2/d. Overall, the Dapeng Cove ecosystem displays net production exceeding respiration, classifying it as an autotrophic system. Additionally, the nitrogen cycle in the sea area is predominantly driven by denitrification. The analysis also revealed that the impact of oyster proliferation on the physical and chemical factors in the surveyed area is relatively weaker than that of surface runoff and groundwater inputs.

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“…Samples were collected at 250-300 m intervals along the center of each area. At each site, triplicate superficial sediments (0-10 cm mud samples) were collected on the sediment surface at 12 sites using a manual Van Veen dredge [12,23]. The sediment samples were sieved with a 2 mm mesh to remove stones and plant roots before being sealed in sterile plastic bags [24].…”

Section: Study Area and Samples Collectionmentioning

confidence: 99%

“…The sieved sediment samples were flash-frozen and kept on boards in liquid nitrogen before being stored at −80 • C in the laboratory [25]. Overlying water samples were collected with Van Dorn bottles washed with 10% HCl and stored in triplicate flasks at −4 • C in a cooler [12].…”

Section: Study Area and Samples Collectionmentioning

confidence: 99%

“…Microbial communities in sediments play an important role in organic remineralization, helping to maintain biogeochemical cycles, release nutrients, and emit greenhouse gases [11,12]. As primary producers, these prokaryotes form an indispensable foundation of the food web of the ARS ecosystem, supporting a diverse range of benthic life, from microbial consumers to animals like zooplanktons, clams, mussels, and shrimps [13].…”

Section: Introductionmentioning

confidence: 99%

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The Effect of the Artificial Reef on the Structure and Function of Sediment Bacterial Community

et al. 2022

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The bacterial community in sediment is sensitive to artificial disturbance, and they respond differently to human disturbance, such as changing the nutrient cycling and energy flow in marine ecosystems. However, little is known about the dynamics and distribution of bacterial community structures in marine sediments and potential biogeochemical functions during the long-time succession in marine ranching. In the present study, we compared the dynamics of the bacterial composition and potential biogeochemical functions of sediment to ten years (TR) and one-year new artificial reef (NR) areas using metagenomic next-generation sequencing technology. Results revealed that NR reduces the Pielou’s evenness and Shannon index. Similarly, nonmetric multidimensional scaling showed that the beta diversity of sediment bacterial communities in NR significantly differed between TR and non-artificial reef areas. Previously, TR biomarkers were frequently associated with organic matter decomposing and assimilating in the organically enriched sediments (i.e., Acinetobacter). The soluble reactive phosphate (SRP) and total phosphorus (TP) concentrations were thought to be the primary driving forces in shaping the microbial community in sediment. Pseudomonas, Lactobacillus, and Ralstonia have a significant positive correlation with SRP, TP, nitrate, and TN, but a negative association with pH, Salinity, Hg, and depth. NR was found to have more negative correlation nodes, indicating that taxa face more competition or predation press. Vibrio served as the module-hubs in the network in all areas. In addition, chemoheterotrophy, aerobic chemoheterotrophy, and fermentation were the three most prominent functions of the three areas, accounting for 59.96% of the relative abundance of the functional annotation. Different bacteria in sediments may change the amount of biogeochemical cycle in the marine ranching ecosystem. These findings can increase our understanding of the succession of the microecosystem for the marine ranching sedimentary environment by revealing how artificial reefs affect the indigenous sediment bacterial community and their responses to environmental variation.

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Spatial Variation and Environmental Parameters Affecting the Abundant and Rare Communities of Bacteria and Archaea in the Sediments of Tropical Urban Reservoirs

Cited by 6 publications

References 73 publications

Temporal patterns of bacterial communities in the Billings Reservoir system

Temporal patterns of bacterial communities in the Billings Reservoir system

Dissolved Inorganic Nutrient Biogeochemistry in an Urbanized Coastal Region: A Study of Dapeng Cove, Shenzhen

The Effect of the Artificial Reef on the Structure and Function of Sediment Bacterial Community

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