Spatial variation in the gastrointestinal microbiome, diet, and nutritional condition of a juvenile flatfish among coastal habitats

Morris, Marc T.; Hauton, Chris; Baylay, Alison J.; Peruzza, Luca; Targett, Timothy E.; Ciotti, Benjamin J.

doi:10.1016/j.marenvres.2021.105413

Cited by 8 publications

(3 citation statements)

References 107 publications

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“…PID21-B) inhibited the growth of the Gram-negative fish pathogen V. ichthyoenteri (Table 1). The gill-derived C. carnis (PG10-B) moderately inhibited this pathogen only when cultured in solid (MA) medium (IC 50 18.6 µg/mL). The opposite held true for the gill-derived Microbacterium sp.…”

Section: Cultivation Extraction and Bioactivity Assessmentsmentioning

confidence: 99%

“…A more recent cultivation-independent report retrieved 304 bacterial genera from plaice gills with sequence reads dominated by the alphaproteobacterial genus Sphingomonas and prevalence of several potential pathogenic genera, such as Shewanella, Pseudoalteromonas, Hafnia, Halomonas, Pasteurella, Pseudomonas, Acinetobacter, Francisella, Vibrio, Listonella, Photobacterium, Mycoplasma, Corynebacterium, Micrococcus, Chryseobacterium, Flavobacterium, Tenacibaculum, Staphylococcus, Bacillus, Lactococcus, Streptococcus and Clostridium [17]. The gut of young wild plaice sampled in Scotland was reportedly dominated by the phyla Proteobacteria, Spirochaetes and Tenericutes with a surprising dominance of Alphaproteobacteria and gut microbial community composition varied depending on the plaice body size, nutritional condition and diet [18]. However, to the best of our knowledge, nothing is known on the potential of plaice-associated microorganisms for production of bioactive secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

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Gut and Gill-Associated Microbiota of the Flatfish European Plaice (Pleuronectes platessa): Diversity, Metabolome and Bioactivity against Human and Aquaculture Pathogens

et al. 2022

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Similar to other marine holobionts, fish are colonized by complex microbial communities that promote their health and growth. Fish-associated microbiota is emerging as a promising source of bioactive metabolites. Pleuronectes platessa (European plaice, plaice), a flatfish with commercial importance, is common in the Baltic Sea. Here we used a culture-dependent survey followed by molecular identification to identify microbiota associated with the gills and the gastrointestinal tract (GIT) of P. platessa, then profiled their antimicrobial activity and metabolome. Altogether, 66 strains (59 bacteria and 7 fungi) were isolated, with Proteobacteria being the most abundant phylum. Gill-associated microbiota accounted for higher number of isolates and were dominated by the Proteobacteria (family Moraxellaceae) and Actinobacteria (family Nocardiaceae), whereas Gram-negative bacterial families Vibrionaceae and Shewanellaceae represented the largest group associated with the GIT. The EtOAc extracts of the solid and liquid media cultures of 21 bacteria and 2 fungi representing the diversity of cultivable plaice-associated microbiota was profiled for their antimicrobial activity against three fish pathogens, human bacterial pathogen panel (ESKAPE) and two human fungal pathogens. More than half of all tested microorganisms, particularly those originating from the GIT epithelium, exhibited antagonistic effect against fish pathogens (Lactococcus garvieae, Vibrio ichthyoenteri) and/or human pathogens (Enterococcus faecium, methicillin-resistant Staphylococcus aureus). Proteobacteria represented the most active isolates. Notably, the solid media extracts displayed higher activity against fish pathogens, while liquid culture extracts were more active against human pathogens. Untargeted metabolomics approach using feature-based molecular networking showed the high chemical diversity of the liquid extracts that contained undescribed clusters. This study highlights plaice-associated microbiota as a potential source of antimicrobials for the control of human and the aquaculture-associated infections. This is the first study reporting diversity, bioactivity and chemical profile of culture-dependent microbiota of plaice.

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Section: Cultivation Extraction and Bioactivity Assessmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gut and Gill-Associated Microbiota of the Flatfish European Plaice (Pleuronectes platessa): Diversity, Metabolome and Bioactivity against Human and Aquaculture Pathogens

et al. 2022

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“…Numerous factors, such as developmental stage, environmental factors (e.g., water salinity and temperature), geographic habitats, and species, can modulate the composition of gut microbiota ( Wang A.R. et al, 2018 ; Zhao et al, 2020 ; Jia et al, 2021 ; Morris et al, 2021 ; Zhang et al, 2021 ). However, information on the effects of TWTP effluents on the gut microbiota of aquatic organisms is limited.…”

Section: Introductionmentioning

confidence: 99%

Effect of Chronic Exposure to Textile Wastewater Treatment Plant Effluents on Growth Performance, Oxidative Stress, and Intestinal Microbiota in Adult Zebrafish (Danio rerio)

et al. 2021

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The ever-increasing production and processing of textiles will lead to greater risks of releasing pollutants into the environment. Textile wastewater treatment plants (TWTPs) effluent are an important source of persistent toxic pollutants in receiving water bodies. The effects of specific pollutants on organisms are usually studied under laboratory conditions, and therefore, comprehensive results are not obtained regarding the chronic combined effects of pollutants under aquatic environmental conditions. Thus, this study aimed to determine the combined effects of TWTP effluents on the growth performance, oxidative stress, inflammatory response, and intestinal microbiota of adult zebrafish (Danio rerio). Exposure to TWTP effluents significantly inhibited growth, exacerbated the condition factor, and increased the mortality of adult zebrafish. Moreover, markedly decreases were observed in the activities of antioxidant enzymes, such as CAT, GSH, GSH-Px, MDA, SOD, and T-AOC, mostly in the intestine and muscle tissues of zebrafish after 1 and 4 months of exposure. In addition, the results demonstrated that TWTP effluent exposure affected the intestinal microbial community composition and decreased community diversity. Slight changes were found in the relative abundance of probiotic Lactobacillus, Akkermansia, and Lactococcus in zebrafish guts after chronic TWTP effluent exposure. The chronic toxic effects of slight increases in opportunistic pathogens, such as Mycoplasma, Stenotrophomonas, and Vibrio, deserve further attention. Our results reveal that TWTP effluent exposure poses potential health risks to aquatic organisms through growth inhibition, oxidative stress impairment of the intestine and muscles, and intestinal microbial community alterations.

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Supplementation with Enterococcus faecium enhances growth performance, intestinal health and immunity of big-belly seahorses (Hippocampus abdominalis) during diet conversion

Spatial variation in the gastrointestinal microbiome, diet, and nutritional condition of a juvenile flatfish among coastal habitats

Cited by 8 publications

References 107 publications

Gut and Gill-Associated Microbiota of the Flatfish European Plaice (Pleuronectes platessa): Diversity, Metabolome and Bioactivity against Human and Aquaculture Pathogens

Gut and Gill-Associated Microbiota of the Flatfish European Plaice (Pleuronectes platessa): Diversity, Metabolome and Bioactivity against Human and Aquaculture Pathogens

Effect of Chronic Exposure to Textile Wastewater Treatment Plant Effluents on Growth Performance, Oxidative Stress, and Intestinal Microbiota in Adult Zebrafish (Danio rerio)

Supplementation with Enterococcus faecium enhances growth performance, intestinal health and immunity of big-belly seahorses (Hippocampus abdominalis) during diet conversion

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