Poyang Lake is the largest freshwater lake in China and is one of the two largest lakes connected with the Yangtze River. It is an overflow lake which absorbs and releases water seasonally. To evaluate the geochemical cycling of nitrogen (N) in Poyang Lake, the spatial distributions of ammonia nitrogen (NH 4 ? -N), nitrate nitrogen (NO 3 --N) and total nitrogen (TN) in sediments and overlying water were measured. The experimental results showed that the average concentrations of TN in the sediment samples, surface water, and bottom water samples from Poyang Lake were 1,107.375 mg kg -1 , 1.998 and 2.237 mg L -1 , respectively. The highest concentrations of TN were measured in the water and sediment obtained from the river tail region south of Poyang Lake, with decreasing pattern from south to north affected by the river input. The contribution of TN to the internal load played an important role on N distribution in the overlying water. The vertical distribution of TN in sediment cores of Poyang Lake showed a decreasing trend with depth. The NH 4 ? -N content in sediment cores initially decreased with depth and then increased slowly. The NO 3 --N content in sediment cores exhibited an inverse trend compared with that of NH 4 ? -N due to the influence of nitrification and denitrification processes. Release kinetic experiments of NH 4? -N demonstrated that the maximum NH 4 ? -N release rate occurred within first 5 min and reached 90-94 % of the maximum value within 0-10 min.