Spatially resolved macromolecular orientation in biological tissues using FT-IR imaging

Kosowska, Karolina; Koziol, Paulina; Liberda, Danuta; Wróbel, Tomasz P.

doi:10.1016/j.clispe.2021.100013

Cited by 7 publications

(13 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Introductionmentioning

confidence: 99%

“…Next, the four-polarization method (4P) has been proposed, which enabled the study of the in-plane organization of molecules in complex systems. The first applications of IR imaging with 4P on polymeric systems − were followed by active compound visualization and recently by human tissue microarray analysis by Koziol et al in 2020. , …”

Section: Introductionmentioning

confidence: 99%

“…The first applications of IR imaging with 4P on polymeric systems 11−14 were followed by active compound visualization 15 and recently by human tissue microarray analysis by Koziol et al in 2020. 16,17 Until now, experiments focused on using a single vibrational band to retrieve the in-plane orientation and internal ordering of samples. 4P is experimentally a simple (no sample tilting) framework that offers much more with proper mathematical treatment.…”

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Super-Resolved 3D Mapping of Molecular Orientation Using Vibrational Techniques

et al. 2022

Self Cite

View full text Add to dashboard Cite

When a sample has an anisotropic structure, it is possible to obtain additional information controlling the polarization of incident light. With their straightforward instrumentation approaches, infrared (IR) and Raman spectroscopies are widely popular in this area. Single-band-based determination of molecular in-plane orientation, typically used in materials science, is here extended by the concurrent use of two vibration bands, revealing the orientational ordering in three dimension. The concurrent analysis was applied to IR spectromicroscopic data to obtain orientation angles of a model polycaprolactone spherulite sample. The applicability of this method spans from high-resolution, diffraction-limited Fourier transform infrared (FT-IR) and Raman imaging to super-resolved optical photothermal infrared (O-PTIR) imaging. Due to the nontomographic experimental approach, no image distortion is visible and nanometer scale orientation domains can be observed. Three-dimensional (3D) bond orientation maps enable in-depth characterization and consequently precise control of the sample’s physicochemical properties and functions.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Super-Resolved 3D Mapping of Molecular Orientation Using Vibrational Techniques

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Next, the fourpolarization method (4P) has been proposed, which enabled the study of the in-plane organization of molecules in complex systems. The first applications of IR imaging with 4P on polymeric systems [11][12][13][14] were followed by active compound visualization 15 and recently by human tissue microarrays analysis by Koziol et al in 2020 16,17 .…”

Section: Mainmentioning

confidence: 99%

Super-resolved 3D mapping of molecular orientation with vibrational techniques

Koziol

Kosowska

Liberda

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

When a sample has an anisotropic structure, it is possible to obtain different information, when changing polarization of incident light. Using polarized light of a single vibrational band to determine the in-plane orientation and internal ordering of a sample is a typical practice in materials science. Acquiring mapping data at four different polarizations with a stationary sample than just at two polarizations offers much more insight into the sample structure with proper mathematical treatment. A concurrent analysis of two vibrational bands with perpendicular transition moment orientations allows the understanding of the orientational ordering in three dimensions. We show here, to the best of our knowledge, the first application of concurrent analysis to IR spectromicroscopy data and obtain orientation angles of a model spherulite polycaprolactone sample. Moreover, we show that this method can be easily applied to high resolution, diffraction limited FT-IR and Raman imaging and even to sub-diffraction limit O-PTIR imaging. Due to the non-tomographic experimental approach, no image distortion is visible and nanometer scale orientation domains can be observed. 3D bond orientation maps will enable in-depth characterization of sample structure in a quantitative manner enabling more precise control of their physicochemical properties and function.

show abstract

“…Kosowska et al 26 later applied the multipolarization method to pancreatic tissue micro arrays. These latter two studies exemplify the spatially erratic orientation of anisotropic components of biological tissues, and thus the importance of applying a multiple-angle polarization approach in similar samples.…”

mentioning

confidence: 99%

Linearly Polarized Infrared Spectroscopy for the Analysis of Biological Materials

Gassner

Vongsvivut

et al. 2023

Appl Spectrosc

View full text Add to dashboard Cite

The analysis of biological samples with polarized infrared spectroscopy (p-IR) has long been a widely practiced method for the determination of sample orientation and structural properties. In contrast to earlier works, which employed this method to investigate the fundamental chemistry of biological systems, recent interests are moving toward “real-world” applications for the evaluation and diagnosis of pathological states. This focal point review provides an up-to-date synopsis of the knowledge of biological materials garnered through linearly p-IR on biomolecules, cells, and tissues. An overview of the theory with special consideration to biological samples is provided. Different modalities which can be employed along with their capabilities and limitations are outlined. Furthermore, an in-depth discussion of factors regarding sample preparation, sample properties, and instrumentation, which can affect p-IR analysis is provided. Additionally, attention is drawn to the potential impacts of analysis of biological samples with inherently polarized light sources, such as synchrotron light and quantum cascade lasers. The vast applications of p-IR for the determination of the structure and orientation of biological samples are given. In conclusion, with considerations to emerging instrumentation, findings by other techniques, and the shift of focus toward clinical applications, we speculate on the future directions of this methodology.

show abstract

Spatially resolved macromolecular orientation in biological tissues using FT-IR imaging

Cited by 7 publications

References 28 publications

Super-Resolved 3D Mapping of Molecular Orientation Using Vibrational Techniques

Super-Resolved 3D Mapping of Molecular Orientation Using Vibrational Techniques

Super-resolved 3D mapping of molecular orientation with vibrational techniques

Linearly Polarized Infrared Spectroscopy for the Analysis of Biological Materials

Contact Info

Product

Resources

About