Spatio-temporal patterns of ensheathing cell differentiation in the rat olfactory system during development

Astic, Liliane; Pellier‐Monnin, Véronique; Godinot, F.

doi:10.1016/s0306-4522(97)00496-x

Cited by 59 publications

(68 citation statements)

References 88 publications

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“…NT R [20,23,61] and S-100 protein [3,44]. In this study double staining with anti-S-100 and Nuclear Yellow (Sigma, St. Louis, MO) was randomly performed in different batches to ascertain the high purity of EC cultures which has been consistently obtained in earlier studies (Fig.…”

Section: Olfactory Ensheathing Cell Culturementioning

confidence: 93%

Cultured olfactory ensheathing cells express nerve growth factor, brain-derived neurotrophic factor, glia cell line-derived neurotrophic factor and their receptors

Woodhall

West

Chuah

2001

Molecular Brain Research

307

209

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In the primary olfactory pathway axons of olfactory neurons (ONs) are accompanied by ensheathing cells (ECs) as the fibres course towards the olfactory bulb. Ensheathing cells are thought to play an important role in promoting and guiding olfactory axons to their appropriate target. In recent years, studies have shown that transplants of ECs into lesions in the central nervous system (CNS) are able to stimulate the growth ofaxons and in some cases restore functional connections. In an attempt to identify a possible mechanism underlying EC support for olfactory nerve growth and CNS axonal regeneration, this study investigated the production of growth factors and expression of corresponding receptors by these cells. Three techniques immunohistochemistry, enzyme linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to assess growth factor expression in cultured ECs. Immunohistochemistry showed that ECs expressed nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and glial cell-line derived neurotrophic factor (GDNF). ELISA confirmed the intracellular presence of NGF and BDNF and showed that, compared to BDNF, about seven times as much NGF was secreted by ECs. RT-PCR analysis demonstrated expression of mRNA for NGF, BDNF, GDNF and neurturin (NTN). In addition, ECs also expressed the receptors trkB, GFRa-1 and GFRa-2. The results of the experiments show that ECs express a number of growth factors and that BDNF in particular could act both in a paracrine and autocrine manner.

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Section: Olfactory Ensheathing Cell Culturementioning

confidence: 93%

Cultured olfactory ensheathing cells express nerve growth factor, brain-derived neurotrophic factor, glia cell line-derived neurotrophic factor and their receptors

Woodhall

West

Chuah

2001

Molecular Brain Research

307

209

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“…We then examined the expression of the Dlx5 lacZ reporter in various sub-populations of MgCs in E14.5 normal embryos, by performing double immunostaining for bgal (Dlx5) and either DCX (Gleeson et al 1999), GnRH (Romanelli et al 2004), or S100 (Astic et al 1998;Tennent and Chuah 1996) on frontal sections of Dlx5 +/lacZ embryos. As seen with Xgal staining, abundant bgal+ cells were found near the medial surface of the OB (Fig.…”

Section: Expression Of Dlx5 In Specific Subpopulation Of Migratory Cellsmentioning

confidence: 99%

The role of Dlx homeogenes in early development of the olfactory pathway

et al. 2007

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Development of the olfactory pathway requires interaction between cells and signals of different origin. Olfactory receptor neurons (ORN) in the olfactory placodes (OP) extend axons towards the forebrain (FB); with innervation taking place at a later time following degradation of the basement membrane. Cells from the OP migrate along ORN axons and differentiate into various elements, including ensheathing and Gonadotropin Releasing Hormone (GnRH)+ cells. The importance of the olfactory connection and migration is highlighted by the severe endocrine phenotype in Kallmann's patients who lack this migratory pathway. Little is known about the genetic control of intrinsic ORN properties. Inactivation of the distalless-related Dlx5 prevents connections between ORNs and FB. Using a grafting approach we show that misguidance and lack of connectivity is due to intrinsic defects in ORN neurites and migratory cells (MgC), and not to environmental factors. These data point to a cellautonomous function of Dlx5 in providing ORN axons with their connectivity properties. Dlx5 also marks a population of early MgC that partly overlaps with the GnRH+ population. In the absence of Dlx5 MgCs of the Dlx5+ lineage migrate, associated with PSA-NCAM+ axons, but fail to reach the FB as a consequence of the lack of axonal connection and not an inability to migrate. These data suggests that Dlx5 is not required to initiate migration and differentiation of MgCs.

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“…The migratory ability of OECs is important for the development of the olfactory system and neural regeneration [11,[17][18][19]. During embryonic development, OECs are derived from olfactory placode, migrate out of the olfactory epithelium along the olfactory axons from the lamina propria, go through the cribriform plate into the cranial cavity and finally form the glia limitations surrounding the olfactory bulb [17].…”

Section: Introductionmentioning

confidence: 99%

Migratory properties of cultured olfactory ensheathing cells by single-cell migration assay

et al. 2008

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Spatio-temporal patterns of ensheathing cell differentiation in the rat olfactory system during development

Cited by 59 publications

References 88 publications

Cultured olfactory ensheathing cells express nerve growth factor, brain-derived neurotrophic factor, glia cell line-derived neurotrophic factor and their receptors

Cultured olfactory ensheathing cells express nerve growth factor, brain-derived neurotrophic factor, glia cell line-derived neurotrophic factor and their receptors

The role of Dlx homeogenes in early development of the olfactory pathway

Migratory properties of cultured olfactory ensheathing cells by single-cell migration assay

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