Spatio-temporal relays control layer identity of direction-selective neuron subtypes in<i>Drosophila</i>

Apitz, Holger; Salecker, Iris

doi:10.1101/262451

Cited by 8 publications

(15 citation statements)

References 75 publications

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“…Together with previous results, our findings suggest that the lamination of T4/T5 axonal outputs occurs via two distinct processes, each controlled by a separate TF. Binary expression of bi is required for lamination of the broad a/b from c/d LoP domains (Apitz and Salecker, 2018), whereas binary expression of grn is required for sublamination of each domain into separate LoP layers. Importantly, perturbation of each TF exclusively disrupts the corresponding lamination step, while not affecting other morphological features of T4/T5 neurons.…”

Section: Discussionmentioning

confidence: 99%

“…RNAi of bi in all T4/T5 neurons results in loss of the c/d domain of the LoP, whereas overexpression results in loss of the a/b domain. In both cases, further sublamination of remaining inner and outer LoP layer pairs still occurs (Apitz and Salecker, 2018). We performed RNAi of grn, which resulted in a different phenotype; whereas distinct a/b and c/d LoP domains were still separated by a pronounced gap and differential expression of Con (a marker for LoP layers c/d), both domains failed to sublaminate into inner and outer layers, instead forming a single layer each (Figure 6a-b).…”

Section: Axon-specific Transcriptional Programs Of T4/t5 Neurons Contmentioning

confidence: 99%

“…We envision that our findings in this system will provide insights into the genetic logic of wiring specificity more broadly in both vertebrate and invertebrate systems. T4/T5 neurons share a common developmental origin, physiological function, and morphology, but differ in their precise wiring patterns and preferred stimulus (Fischbach and Dittrich, 1989;Maisak et al, 2013;Apitz and Salecker, 2018;Pinto-Teixeira et al, 2018;Shinomiya et al, 2019). T4/T5s can be classified into two quartets of subtypes based on dendritic inputs: the four T4 subtypes share a common set of dendritic inputs in the medulla, and the four T5 neurons share a different set of dendritic inputs in the lobula (Figure 1a-c).…”

Section: Introductionmentioning

confidence: 99%

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Modular transcriptional programs separately define axon and dendrite connectivity

Kurmangaliyev

Yoo

LoCascio

et al. 2019

Preprint

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Patterns of synaptic connectivity are remarkably precise and complex. Single-cell RNA sequencing has revealed a vast transcriptional diversity of neurons. Nevertheless, a clear logic underlying the transcriptional control of neuronal connectivity has yet to emerge. Here, we focused on Drosophila T4/T5 neurons, a class of closely related neuronal subtypes with different wiring patterns. Eight subtypes of T4/T5 neurons are defined by combinations of two patterns of dendritic inputs and four patterns of axonal outputs. Single-cell profiling during development revealed distinct transcriptional programs defining each dendrite and axon wiring pattern. These programs were defined by the expression of a few transcription factors and different combinations of cell surface proteins. Gain and loss of function studies provide evidence for independent control of different wiring features. We propose that modular transcriptional programs for distinct wiring features are assembled in different combinations to generate diverse patterns of neuronal connectivity.

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Section: Discussionmentioning

confidence: 99%

Section: Axon-specific Transcriptional Programs Of T4/t5 Neurons Contmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Modular transcriptional programs separately define axon and dendrite connectivity

Kurmangaliyev

Yoo

LoCascio

et al. 2019

Preprint

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“…As with LC11, we found that T2 and T3 both respond strongly to a small moving object, show an intermediate response to a bar, and almost no response to wide-field grating motion ( Figure 3H). Because the columnar T2 and T3 neurons are developmentally related to the motion detecting neurons, T4 and T5 (Apitz and Salecker, 2018), and share some of the same synaptic inputs from the medulla (Shinomiya et al, 2019;Takemura et al, 2015), we made direct comparisons among all four cell types.…”

Section: T2 and T3 Neurons Innervate Lc11 Dendritesmentioning

confidence: 99%

Inhibitory Interactions and Columnar Inputs to an Object Motion Detector in Drosophila

et al. 2020

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Graphical Abstract Highlights d LC11 neurons are suppressed by motion and flicker in the local surround d LC11 expresses a GABA receptor required for normal object detection d T2/T3 respond to ON and OFF components of object motion and flicker d T3 neurons excite LC11 and are required for normal LC11 function SUMMARYThe direction-selective T4/T5 cells innervate opticflow processing projection neurons in the lobula plate of the fly that mediate the visual control of locomotion. In the lobula, visual projection neurons coordinate complex behavioral responses to visual features, however, the input circuitry and computations that bestow their feature-detecting properties are less clear. Here, we study a highly specialized small object motion detector, LC11, and demonstrate that its responses are suppressed by local background motion. We show that LC11 expresses GABA-A receptors that serve to sculpt responses to small objects but are not responsible for the rejection of background motion. Instead, LC11 is innervated by columnar T2 and T3 neurons that are themselves highly sensitive to small static or moving objects, insensitive to wide-field motion and, unlike T4/T5, respond to both ON and OFF luminance steps.

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“…A recent study by Pinto-Teixeira et al [2], along with two related studies [3,4], illustrates how simple developmental processes can be combined to produce, differentiate, and wire a large population of neurons with complex synaptic connections ( Figure 1).…”

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confidence: 98%

Developmental Biology: Neurons That Divide Together Wire Together

Pang

Clandinin

2018

Current Biology

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Spatio-temporal relays control layer identity of direction-selective neuron subtypes inDrosophila

Cited by 8 publications

References 75 publications

Modular transcriptional programs separately define axon and dendrite connectivity

Modular transcriptional programs separately define axon and dendrite connectivity

Inhibitory Interactions and Columnar Inputs to an Object Motion Detector in Drosophila

Developmental Biology: Neurons That Divide Together Wire Together

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