Spatiotemporal Expression and Bioinformatic Analyses of the HD-Zip Transcription Factor Family in Larix olgensis

An, Peiqi; Cao, Qing; Wang, Chen; Wang, Junhun; Zhang, Hanguo; Zhang, Lei

doi:10.1007/s11105-020-01244-9

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…Because it is difficult to cultivate larch in vitro regenerators, difficult to root, and long growth cycle, a large number of seedlings cannot be obtained in a short time 46 . Therefore, in order to clarify the function of LoHDZ2 , LoHDZ2 was transformed into model plant tobacco 47 , and transgenic tobacco was successfully obtained. After cultivating transgenic tobacco plants, the phenotypic characters and physiological and biochemical indexes of different transgenic lines were determined.…”

Section: Discussionmentioning

confidence: 99%

Genetic transformation of LoHDZ2 and analysis of its function to enhance stress resistance in Larix olgensis

Qin

Zhao

et al. 2022

Sci Rep

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To study the function of LoHDZ2 in larch, we first constructed a VB191103-LoHDZ2::GUS overexpression vector. Through Agrobacterium-mediated infection, the expression vector was transferred into a larch embryogenic cell line. A stable resistant cell line was subsequently screened, and mature embryos were induced to grow until they developed into seedlings. Antagonistic cell lines were identified at both the DNA and RNA levels. The transgenic cell lines were then subjected to GUS staining, and transgenic cell lines were ultimately identified and obtained. These transgenic cell lines were sequenced to identify differentially expressed genes, and a cluster analysis was performed. The resistant cell lines were cultured under stress conditions involving 20% PEG6000 and 200 mM NaCl proliferation media (1/10-BM). After the stress treatment, the contents of peroxidase (POD), malondialdehyde (MDA) and superoxide dismutase (SOD) in both wild-type and transgenic cell lines were measured. The results are summarized below: (1) When the specific fragment of the target gene in the genome of the resistant cell line was amplified. At the RNA level, the expression of the fragment in four resistant lines increased. In addition, GUS staining showed a blue reaction, indicating that LoHDZ2 was successfully integrated into the larch embryonic cell lines. (2) To verify the accuracy and reliability of the transcriptome data, 10 differentially expressed genes (5 upregulated and 5 down regulated genes) were subjected to qRT-PCR verification. The results showed that the expression trend of the 10 differentially expressed genes was the same as that revealed by RNA-Seq, indicating that the transcriptome data were reliable. (3) The transcriptome sequencing showed that 176 genes were upregulated and that 140 genes were down regulated. Through GO enrichment analysis and KEGG metabolic pathway analysis, the screened differentially expressed genes were related to biological processes such as larch metabolism and response to stimuli, indicating that these genes may be closely involved in the regulation of the larch response to external stimuli, including heat stress, drought stress, metal ion stress and bacterial infection, and may participate in the growth process. (4) After 20% PEG6000 treatment, the POD enzyme activity of the transgenic cell line was greater than that of the wild-type; this activity could effectively remove the amount of peroxide produced. The MDA content of the transgenic cell lines was lower than that of the wild-type cell lines, and the accumulation degree of harmful substances was low, indicating that the degree of oxidative damage of the transgenic cell lines was lower than that of the wild-type cell lines. The SOD content of the transgenic cell lines was lower than that of the wild-type cell lines, indicating that the drought resistance of the transgenic cell lines was enhanced. After 200 mM NaCl treatment, although the increase in SOD content was not obvious, the same trend was detected, indicating that the resistance of the transgenic cell lines was indeed stronger than that of the wild-type cell lines. According to the results of previous experiments, after this gene was overexpressed in tobacco, the transformed plants showed obvious dwarfing, which may indicate that the stress resistance of the plant was enhanced. In conclusion, a transgenic larch cell line was successfully obtained, and transgenic larch seedlings were successfully induced. LoHDZ2 may participate in the response of plants to the external environment, and may participate in the growth and development of Larixolgensis by affecting plant metabolic pathways.

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Section: Discussionmentioning

confidence: 99%

Genetic transformation of LoHDZ2 and analysis of its function to enhance stress resistance in Larix olgensis

Qin

Zhao

et al. 2022

Sci Rep

Self Cite

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“…SOPMA and SWISS MODEL online software were used to analyze the secondary and tertiary structures of proteins and the WOLF PSORT website was used to predict subcellular localization. The bioinformatics analysis website refers to the article [24] of Peiqi An et al, (Table 1). The temporal and spatial expression pattern of the WRKY gene in larch and its participation in abiotic stress response were analyzed by real-time fluorescence quantitative PCR.…”

Section: Bioinformatics Analysismentioning

confidence: 99%

Expression Pattern Analysis of Larch WRKY in Response to Abiotic Stress

Wang

Zhao

Zhang

et al. 2022

Forests

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Larix olgensis is one of the most common tree species in Northeast China; it has the advantages of fast growth and good wood properties. In order to accelerate larch molecular breeding and to provide good candidate genes for larch improvement, based on the existing transcriptome data of Larix olgensis, four WRKY family genes with complete CD regions were obtained by BLAST comparison on the NCBI website. The results of bioinformatics analysis and gene expression after abiotic stress showed that there were some differences in the expression of WRKY1, WRKY2, WRKY3 and WRKY4 in roots, stems and leaves under each treatment. Under the treatment of a 40% PEG6000 solution (polyethylene glycol), the expression of WRKY2 was significantly up-regulated in each time period and WRKY1, WRKY3 and WRKY4 were down-regulated in varying degrees compared with the control group, indicating that they were involved in the response to drought stress. Under the treatment of the 0.2mol/L NaCl solution, the expression of WRKY2 was up-regulated in roots, stems and leaves. The expression amount and the expression trend of the other three genes were different in roots, stems and leaves under different treatment durations, indicating that they were also involved in a salt-stress response. Under the treatment of the 0.1 mol/L NaHCO3 solution, the expression of WRKY4 was significantly down-regulated in all time periods, while WRKY2 was significantly up-regulated. The other two genes were regulated to a certain extent, indicating that they also had a physiological response under alkaline conditions. These results lay a foundation for the study of gene function of these four WRKY transcription factors.

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Genome wide characterization and identification of candidate HD-Zip genes involved in prickle density in Rosa roxburghii

Qin,

Nan,

et al. 2024

Scientia Horticulturae

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Spatiotemporal Expression and Bioinformatic Analyses of the HD-Zip Transcription Factor Family in Larix olgensis

Cited by 3 publications

References 41 publications

Genetic transformation of LoHDZ2 and analysis of its function to enhance stress resistance in Larix olgensis

Genetic transformation of LoHDZ2 and analysis of its function to enhance stress resistance in Larix olgensis

Expression Pattern Analysis of Larch WRKY in Response to Abiotic Stress

Genome wide characterization and identification of candidate HD-Zip genes involved in prickle density in Rosa roxburghii

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