Spatiotemporal expression pattern of Myt/NZF family zinc finger transcription factors during mouse nervous system development

Abstract

Background: Three members of the Myt/NZF family of transcription factors are involved in many processes of vertebrate development. Several studies have reported that Myt1/NZF-2 has a regulatory function in the development of cultured oligodendrocyte progenitors or in neuronal differentiation during Xenopus primary neurogenesis. However, little is known about the proper function of Myt/NZF family proteins during mammalian nervous system development. To assess the possible function of Myt/NZF transcription factors in mammalian neuronal differentiation, we determined the comparative spatial and temporal expression patterns of all three types of Myt/NZF family genes in the embryonic mouse nervous system using quantitative reverse transcriptase polymerase chain reaction and in situ hybridization. Results: All three Myt/NZF family genes were extensively expressed in developing mouse nervous tissues, and their expression was transient. NZF-1 was expressed later in post-mitotic neurons. NZF-2 was initially expressed in neuronal cells a little earlier than NZF-3. NZF-3 was initially expressed in neuronal cells, just after proliferation was complete. Conclusion: These expression patterns suggest that the expression of NZF family genes is spatially and temporally regulated, and each Myt/NZF family gene may have a regulatory function in a specific phase during neuronal differentiation. Developmental Dynamics 243:588-600, 2014. Developmental DynamicsABBREVIATIONS APa archipallium BAM Brn2, Ascl1, and Myt1l BAMN Brn2, Ascl1, Myt1l, and NeuroD1 BG basal ganglia BrdU bromodeoxyuridine CA1, CA3 cornu ammonis fields CBL cerebellum CNS central nervous system CP cortical plate cSp cervical spinal cord CTX cortex DG dentate gyrus DIG digoxigenin dpc days post coitum DRG dorsal root ganglion DTe dorsal telencephalon EGL external germinal layer of the cerebellum fIC fibers of the internal capsule HC hippocampus HT hypothalamus IC internal capsule iCo inferior colliculus iNBL inner neuroblastic layer of the neural retina Is isthmus ISH in situ hybridization IXg glossopharyngeal ganglia IZ intermediate zone LDN lateral deep nucleus LGE lateral ganglionic eminence Me medulla MGE medial ganglionic eminence ML mantle layer MN motor neuron Myt myelin transcription factor NZF neural zinc finger oNBL outer neuroblastic layer of the neural retina OpV optic vesicle OtV otic vesicle Pal pallidum PBS phosphate-buffered saline POA preoptic area PP preplate Ps pons sCo superior colliculus SVZ subventricular zone Str striatum sXg superior ganglion of the vagus nerve Teg tegmentum TH thalamus UTR untranslated region Vg trigeminal ganglion VIIg facial ganglion VIIIg vestibulocochlear ganglion VII-VIII facio-acoustic neural crest complex VTe ventral telencephalon VZ ventricular zone WM white matter Xg vagus ganglia.

Mutations inMYT1, encoding the myelin transcription factor 1, are a rare cause of OAVS

“…In zebrafish, one MYT1 homologue, myt1a , presented an expression pattern similar to that of the MYT1/NZF family in the developing mouse hindbrain 34. Specific expression in zebrafish olfactory bulbs was confirmed 35.…”

Myt1l safeguards neuronal identity by actively repressing many non-neuronal fates

“…5 Department of Genetics, Stanford University, Stanford, California 94305, USA. 6 Division of Functional Genomics and Systems Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden. 7 Genome Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany.…”

Refinement of the critical 2p25.3 deletion region: the role of MYT1L in intellectual disability and obesity

“…18,19 In addition, we and others have shown that the expression of MYT1L is restricted to neuronal tissues (Figure 3), with peaks during neurogenesis. 3,9,10 To investigate in more detail the temporal and spatial expression of MYT1L, we analyzed the expression of the orthologs in zebrafish. According to the Zebrafish Model Organism Database (http://zfin.org/), two zebrafish orthologs are present for MYT1L, that is, myt1la and myt1lb.…”