Spatiotemporal resolution of BDNF neuroprotection against glutamate excitotoxicity in cultured hippocampal neurons

Melo, Carlos; Okumoto, Sakiko; Gomes, João R.; Baptista, Márcio S.; Bahr, Ben A.; Frommer, Wolf B.; Duarte, Carlos B.

doi:10.1016/j.neuroscience.2013.01.054

Cited by 29 publications

(15 citation statements)

References 99 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Under neurotoxic conditions at the cell level, the reduction in neurofilament immunolabeling associated with cytoskeleton damage was prevented by stiripentol and BDNF. These results confirm the neuroprotective potential of BDNF in addition to its neurotrophic properties previously described from various in vitro experiments (Melo et al, ; Bothwell, ; Van Kanegan et al, ). They also demonstrate the neuroprotective effects of stiripentol.…”

Section: Discussionsupporting

confidence: 91%

Neuroprotective activity of stiripentol with a possible involvement of voltage‐dependent calcium and sodium channels

Verleye

Buttigieg²,

Steinschneider³

2015

J of Neuroscience Research

View full text Add to dashboard Cite

A growing body of data has shown that recurrent epileptic seizures may be caused by an excessive release of the excitatory neurotransmitter glutamate in the brain. Glutamatergic overstimulation results in massive neuronal influxes of calcium and sodium through N-methyl-D-aspartate (NMDA), α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, and kainic acid glutamate subtype receptors and also through voltage-gated calcium and sodium channels. These persistent and abnormal sodium and calcium entry points have deleterious consequences (neurotoxicity) for neuronal function. The therapeutic value of an antiepileptic drug would include not only control of seizure activity but also protection of neuronal tissue. The present study examines the in vitro neuroprotective effects of stiripentol, an antiepileptic compound with γ-aminobutyric acidergic properties, on neuronal-astroglial cultures from rat cerebral cortex exposed to oxygen-glucose deprivation (OGD) or to glutamate (40 µM for 20 min), two in vitro models of brain injury. In addition, the affinity of stiripentol for the different glutamate receptor subtypes and the interaction with the cell influx of Na(+) and of Ca(2+) enhanced by veratridine and NMDA, respectively, are assessed. Stiripentol (10-100 µM) included in the culture medium during OGD or with glutamate significantly increased the number of surviving neurons relative to controls. Stiripentol displayed no binding affinity for different subtypes of glutamate receptors (IC50 >100 µM) but significantly blocked the entry of Na(+) and Ca(2+) activated by veratridine and NMDA, respectively. These results suggest that Na(+) and Ca(2+) channels could contribute to the neuroprotective properties of sitiripentol.

show abstract

Section: Discussionsupporting

confidence: 91%

Neuroprotective activity of stiripentol with a possible involvement of voltage‐dependent calcium and sodium channels

Verleye

Buttigieg²,

Steinschneider³

2015

J of Neuroscience Research

View full text Add to dashboard Cite

show abstract

“…These primary cultures can be augmented by coating the bottom of the culture dish with extracellular matrix components, including laminin, fibronectin, cadherin, and collagen, through which axons can extend 74 . In addition, neuroprotective agents, including BDNF 75 76 , nerve growth factor 77 , and CTNF 78 79 , can easily be provided to the cells uniformly by adding them to the culture medium to further support neuron and axonal growth.…”

Section: Discussionmentioning

confidence: 99%

Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells

Tanaka

Yamaguchi

Tamalu

et al. 2015

Sci Rep

112

139

View full text Add to dashboard Cite

We generated self-induced retinal ganglion cells (RGCs) with functional axons from human induced pluripotent stem cells. After development of the optic vesicle from the induced stem cell embryoid body in three-dimensional culture, conversion to two-dimensional culture, achieved by supplementation with BDNF, resulted in differentiation of RGCs at a rate of nearly 90% as indicated by a marginal subregion of an extruded clump of cells, suggesting the formation of an optic vesicle. Axons extended radially from the margin of the clump. Induced RGCs expressed specific markers, such as Brn3b and Math5, as assessed using by quantitative PCR and immunohistochemistry. The long, prominent axons contained neurofilaments and tau and exhibited anterograde axonal transport and sodium-dependent action potentials. The ability to generate RGCs with functional axons uniformly and at a high rate may contribute to both basic and clinical science, including embryology, neurology, pathognomy, and treatment of various optic nerve diseases that threaten vision.

show abstract

“…Corroborating with such idea other authors found that within patients with cognitive impairments, those with higher BDNF levels exhibited better performance in cognitive tasks in comparison to those exhibiting lower levels [48]. Such findings could reflect some kind of neuroprotective effects of BDNF [49,50]. In addition, our data suggest that the relationship between peripheral BDNF fluctuations and the storage and retrieval memory processes may be one an intriguing aspect in clinical and neuropsychological outcomes of crack/cocaine dependents.…”

Section: Discussionmentioning

confidence: 85%

Brain-Derived Neurotrophic Factor and Delayed Verbal Recall in Crack/Cocaine Dependents

et al. 2015

View full text Add to dashboard Cite

Background/Aims: Considering the role of brain-derived neurotrophic factor (BDNF) in memory processes and its peripheral response during the detoxification of cocaine, the aim of this study was to investigate whether plasma BDNF levels could be related to memory performance in women with crack/cocaine dependence. Methods: Twenty-five abstinent female crack/cocaine users (CCD) and 25 unmedicated healthy women (HW), carefully matched for age and years of formal education, were assessed regarding memory performance. Logical Memory was used to assess the immediate verbal recall (IVR), delayed verbal recall (DVR) and memory retention. Plasma BDNF levels were measured by Elisa immunoassay. Beck Depression Inventory was used to assess the severity of depressive symptoms, and the Cocaine Selective Severity Assessment the severity of cocaine abstinence symptoms. Results: The CCD group had lower DVR scores and higher plasma BDNF levels when compared to HW group. In addition, a linear regression model showed that BDNF levels predicted DVR scores within CCD group independently of depressive symptoms (R = 0.51; R² = 0.26; t(22) = 4.025, p = 0.03). Conclusion: Despite higher plasma BDNF levels, crack users exhibited memory impairments when compared to healthy women. Specifically, peripheral BDNF levels predicted better cognitive performance only within individuals who already had cognitive impairment.

show abstract

Spatiotemporal resolution of BDNF neuroprotection against glutamate excitotoxicity in cultured hippocampal neurons

Cited by 29 publications

References 99 publications

Neuroprotective activity of stiripentol with a possible involvement of voltage‐dependent calcium and sodium channels

Neuroprotective activity of stiripentol with a possible involvement of voltage‐dependent calcium and sodium channels

Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells

Brain-Derived Neurotrophic Factor and Delayed Verbal Recall in Crack/Cocaine Dependents

Contact Info

Product

Resources

About