Spatiotemporal variations, assembly processes, and co-occurrence patterns of particle-attached and free-living bacteria in a large drinking water reservoir in China

Abstract

Particle-attached (PA) and free-living (FL) bacterial communities are sensitive to pollutant concentrations and play an essential role in biogeochemical processes and water quality maintenance in aquatic ecosystems. However, the spatiotemporal variations, assembly processes, co-occurrence patterns, and environmental interactions of PA and FL bacteria in drinking water reservoirs remain as yet unexplored. To bridge this gap, we collected samples from 10 sites across four seasons in Lake Tianmu, a large drinking water reservoir in China. Analysis of 16S rRNA gene libraries demonstrated spatiotemporal variations in bacterial diversity and identified differences in bacterial community composition (BCC) between PA and FL lifestyles. Capacity for nitrogen respiration, nitrogen fixation, and nitrate denitrification was enriched in the PA lifestyle, while photosynthesis, methylotrophy, and methanol oxidation were enriched in the FL lifestyle. Deterministic processes, including interspecies interactions and environmental filtration, dominated the assembly of both PA and FL bacterial communities. The influence of environmental filtration on the FL community was stronger than that on the PA community, indicating that bacteria in the FL lifestyle were more sensitive to environmental variation. Co-occurrence patterns and keystone taxa differed between PA and FL lifestyles. The ecological functions of keystone taxa in the PA lifestyle were associated with the supply and recycling of nutrients, while those in FL were associated with the degradation of complex pollutants. PA communities were more stable than FL communities in the face of changing environmental conditions. Nutrients (e.g., TDN and NO3–) and abiotic and biotic factors (e.g., WT and Chl-a) exerted positive and negative effects, respectively, on the co-occurrence networks of both lifestyles. These results improve our understanding of assembly processes, co-occurrence patterns, and environmental interactions within PA and FL communities in a drinking water reservoir.

Seasonal changes of prokaryotic microbial community structure in Zhangjiayan Reservoir and its response to environmental factors

“…PCR amplification and high-throughput sequencing were performed using bacterial primers 338F: 5′-ACTCCTACGGGAGGCAGCA-3′ and 806R: 5′-GGACTACHVGGGTWTCTAAT-3′ 15 . The PCR protocol was as follows: 30 cycles of initial denaturation at 95 °C for 30 s, denaturation at 98 °C for 15 s, annealing at 55 °C for 30 s, extension at 72 °C for 30 s, and then extension at 72 °C for 10 min.…”

The Role of Stakeholders’ Understandings in Emerging Antimicrobial Resistance: A One Health Approach

“…For example, the presence of copper and zinc on plastic surfaces can promote the binding of ARGs related to resistance against certain antibiotics. Organic pollutants like polycyclic aromatic hydrocarbons have been found to exert selective pressure on the transfer of ARGs through mechanisms like co-selection or cross-selection [82,84].…”

“…It is therefore essential to deepen our knowledge of the environmental behaviour of MPs and their role in the transmission of ARGs by studying the MPs resistome and how much of this is shared with the surrounding environment [23,24], to understand the potential risk of exposure for humans [83][84][85][86][87]. The importance of the environment and food of both animal and plant origin in the emergence of emerging diseases, such as SARS and avian influenza, allowed the term 'One Health' to be coined in 2003 and laid the foundations for the importance of collaboration between different professions to respond with a strategic approach to prevention and surveillance of emerging diseases [3,8,70].…”

Marine Bacterioplankton Community Dynamics and Potentially Pathogenic Bacteria in Seawater around Jeju Island, South Korea, via Metabarcoding

“…In South Korea, the summer-autumn rainy season corresponds to the period from mid-August to mid-September, when typhoons affect the Korean Peninsula directly or indirectly [22]. The large input of rainwater during this period rapidly reduces seawater salinity, and this affects the microbial community in this area [23,24].…”