Special Issue “Aquaporins in Brain Disease”

Echevarría, Miriam; García-Miranda, Pablo

doi:10.3390/ijms25063513

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…Our most recent studies have demonstrated that AQP4 expression is clearly associated with myelination in areas of white matter, as in the CC and cerebellum in the brains of mice [6,7]. Additionally, we identified a population of CD11c+ microglia in the mouse periaqueductal zone [8], similar to the one detected by other authors in the CC and cerebellum, which had been reported to be very important in maintaining these tissues' myelinisation and neurogenesis in WT mice [4].…”

Section: Discussionsupporting

confidence: 67%

“…Recently, we demonstrated that aquaporin-4 (AQP4) expression is clearly associated with the early cell differentiation process that leads to myelination in areas of white matter in mice brains, such as in the CC and cerebellum [6,7]. We also identified, for the first time, a population of CD11c+ microglia in the periaqueductal zone of AQP4-knockout (KO) animals similar to that detected by other authors in the CC and cerebellum, which is believed to be important for maintaining the myelinisation and neurogenesis of these tissues in WT mice [4].…”

Section: Introductionmentioning

confidence: 99%

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Absence of AQP4 Prolongs the Presence of a CD11c+ Microglial Population during Postnatal Corpus Callosum Development

Mayo,

González-Vinceiro,

Hiraldo-González

et al. 2024

Preprint

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Aquaporin-4 (AQP4) expression is associated with development of congenital hydrocephalus due to its structural role in the ependymal membrane. Gene expression analysis of periaqueductal tissue in AQP4-knockout (KO) mice at 11 days of age (P11) showed modification in ependymal cell adhesion and ciliary protein expression that could alter cerebrospinal fluid homeostasis. A microglial subpopulation of CD11c+ cells were overexpressed in the periaqueductal tissue of mice that did not develop hydrocephalus, suggesting a possible protective effect. Here, we verified the location of this CD11c+ expression in the corpus callosum (CC) and cerebellum of AQP4-KO mice and analysed its time course. Immunofluorescence labelling of the CD11c protein in the CC and cerebellum of WT and KO animals at P3, P5, P7 and P11 confirmed an expanded presence of these cells in both tissues of the KO animal; CD11c+ cells appeared at P3 and reached a peak at P11, whereas in the WT animal they appeared at P5, reached their peak at P7 and were undetectable by P11. The gene expression analysis in the CC samples at P11 confirmed the presence of CD11c+ microglial cells in this tissue. Among the more than 4000 overexpressed genes, Spp1 stood out with the highest differential gene expression (600), with other genes such as Gpnmb, Itgax, Cd68 and Atp6v0d2 also identified as overexpressed. Therefore, CD11c+ cells appear to be necessary for normal corpus callosum development during postnatal life, and absence of AQP4 prolonged its expression in this tissue.

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Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Absence of AQP4 Prolongs the Presence of a CD11c+ Microglial Population during Postnatal Corpus Callosum Development

Mayo,

González-Vinceiro,

Hiraldo-González

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Absence of Aquaporin-4 (AQP4) Prolongs the Presence of a CD11c+ Microglial Population during Postnatal Corpus Callosum Development

Mayo,

González-Vinceiro,

Hiraldo-González

et al. 2024

IJMS

View full text Add to dashboard Cite

Aquaporin-4 (AQP4) expression is associated with the development of congenital hydrocephalus due to its structural role in the ependymal membrane. Gene expression analysis of periaqueductal tissue in AQP4-knockout (KO) mice at 11 days of age (P11) showed a modification in ependymal cell adhesion and ciliary protein expression that could alter cerebrospinal fluid homeostasis. A microglial subpopulation of CD11c+ cells was overexpressed in the periaqueductal tissue of mice that did not develop hydrocephalus, suggesting a possible protective effect. Here, we verified the location of this CD11c+ expression in the corpus callosum (CC) and cerebellum of AQP4-KO mice and analysed its time course. Immunofluorescence labelling of the CD11c protein in the CC and cerebellum of WT and KO animals at P3, P5, P7 and P11 confirmed an expanded presence of these cells in both tissues of the KO animal; CD11c+ cells appeared at P3 and reached a peak at P11, whereas in the WT animal, they appeared at P5, reached their peak at P7 and were undetectable by P11. The gene expression analysis in the CC samples at P11 confirmed the presence of CD11c+ microglial cells in this tissue. Among the more than 4000 overexpressed genes, Spp1 stood out with the highest differential gene expression (≅600), with other genes, such as Gpnmb, Itgax, Cd68 and Atp6v0d2, also identified as overexpressed. Therefore, CD11c+ cells appear to be necessary for normal corpus callosum development during postnatal life, and the absence of AQP4 prolonged its expression in this tissue.

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Special Issue “Aquaporins in Brain Disease”

Abstract: Water is an abundant and important component of the human brain, the homeostasis of which is rigorously controlled [...]

Cited by 2 publications

References 9 publications

Absence of AQP4 Prolongs the Presence of a CD11c+ Microglial Population during Postnatal Corpus Callosum Development

Absence of AQP4 Prolongs the Presence of a CD11c+ Microglial Population during Postnatal Corpus Callosum Development

Absence of Aquaporin-4 (AQP4) Prolongs the Presence of a CD11c+ Microglial Population during Postnatal Corpus Callosum Development

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