Special Issue: Dynamics and Nano-Organization in Plasma Membranes

Díaz, Yenisleidy de las Mercedes Zulueta; Arnspang, Eva C.

doi:10.3390/membranes11110828

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…Both membrane-embedded and -associated proteins and peptides play a critical role in cell functioning, particularly in cellular signal transduction. Often, for the cells to execute specific functions, the actions of these proteins need to be regulated in an orchestrated manner [ 4 , 8 , 9 , 10 ]. Most studies related to cell membrane functions are devoted to investigating the proteins involved in various signaling pathways [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Membrane Partitioning of TEMPO Discriminates Human Lung Cancer from Neighboring Normal Cells

Gasymov,

Bakhishova,

Aslanov

et al. 2024

Acta Naturae

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The plasma membranes of normal and cancer cells of the lung, breast, and colon tissues show considerably different lipid compositions that greatly influence their physicochemical properties. Partitioning of the spin probe 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) into the membranes of human lung normal and carcinoma cells was assessed by EPR spectroscopy to estimate the impact of the lipid compositions. The goal was to reveal potential strategies for cancer therapy attributable to the membrane properties. The study was conducted at pH values of 7.3 and 6.2, relevant to the microenvironments of normal and cancer cells, respectively. The TEMPO partitioning was examined in the temperature interval of 283–317K to reveal the efficacy of local hyperthermia used in chemotherapy. Results indicate that the TEMPO partitioning coefficient for the membranes of human lung carcinoma cells is significantly higher compared with that of neighboring normal cells. Increased partition coefficients were observed at relatively higher temperatures in both normal and cancer cells. However, compared to the normal cells, the cancer cells demonstrated higher partition coefficients in the studied temperature range. The data obtained with C12SL (spin-labeled analog of lauric acid) indicate that increased membrane dynamics of the cancer cells is a possible mechanism for enhanced partitioning of TEMPO. Free energy values for partitioning estimated for pH values of 6.2 and 7.3 show that TEMPO partitioning requires 30% less energy in the cancer cells at pH 7.3. TEMPO and its derivatives have previously been considered as theranostic agents in cancer research. Data suggest that TEMPO derivatives could be used to test if complementary alkalization therapy is effective for cancer patients receiving standard chemotherapy with local hyperthermia.

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Section: Introductionmentioning

confidence: 99%

Membrane Partitioning of TEMPO Discriminates Human Lung Cancer from Neighboring Normal Cells

Gasymov,

Bakhishova,

Aslanov

et al. 2024

Acta Naturae

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Benzoate Group Attachment to Tempo Provides Enhanced Discrimination of Liposomes Fabricated Using Human Lung Normal and Carcinoma Cells

Gasymov

Bakhishova

Gasanova

et al. 2022

Russian Journal of Biological Physics and Chemisrty

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It is widely accepted that the lipid compositions of the plasma membranes of healthy and cancer cells significantly differ from each other. During the cancer progression, cancer cells change the lipid constituent of the membranes resulting in the loss of lipid asymmetry between the membrane leaflets. Consequently, physicochemical properties of the cell membranes are also changed in response to altered lipid organization. Partitioning of the spin probe 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) into the membranes of the cells has broadly been applied to characterize membrane properties of various cells in health and disease conditions. In this work, we used liposomes fabricated using lipids extracted from normal and carcinoma cells. This system permits the determination of the properties of the healthy and cancer cell membranes provided exclusively by its lipid components. Application of TEMPO-benzoate, in which the benzoate group is attached to the TEMPO, indicates significantly enhanced discrimination of liposomes between cancer and normal cells. Partitioning experiments with TEMPO-benzoate revealed relatively enhanced incorporation efficiency for liposomes of cancer cells. On the contrary, TEMPO incorporation efficiency in the same liposomes of cancer cells was not much different compared to healthy cells. Data indicate that TEMPO-benzoate as a probe is more suitable than TEMPO to discriminate cancer cells from healthy cells. Free energy gain observed for TEMPO-benzoate resulted mainly from the hydrophobic effect of the benzoate group.

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Special Issue: Dynamics and Nano-Organization in Plasma Membranes

Abstract: Cell membranes develop extraordinarily complex lipids and proteins geared to perform functions required by cells [...]

Cited by 2 publications

References 10 publications

Membrane Partitioning of TEMPO Discriminates Human Lung Cancer from Neighboring Normal Cells

Membrane Partitioning of TEMPO Discriminates Human Lung Cancer from Neighboring Normal Cells

Benzoate Group Attachment to Tempo Provides Enhanced Discrimination of Liposomes Fabricated Using Human Lung Normal and Carcinoma Cells

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