Special issue for the International Conference on Electron Paramagnetic Resonance Spectroscopy and Imaging of Biological Systems (EPR-2017)

Khramtsov, Valery V.; Davies, Michael J.

doi:10.1080/10715762.2018.1445852

Cited by 1 publication

(1 citation statement)

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“…Our data demonstrates the involvement of oxidative stress in thyroid tumors, consistent with previous literature, utilizing the novel method of EPR spectroscopy [9]. Compared to other methods of detecting oxidative stress, EPR spectroscopy allows us to obtain a direct measurement of the primary species, rather than its effect [10]. However, the downstream effects exerted by oxidative stress on the biochemical and metabolic pathways involved in thyroid cancer remain unknown.…”

Section: Introductionsupporting

confidence: 89%

Metabolomic Reprogramming Detected by 1H-NMR Spectroscopy in Human Thyroid Cancer Tissues

Metere

Graves

Chirico

et al. 2020

Biology

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Thyroid cancer cells demonstrate an increase in oxidative stress and decreased antioxidant action, but the effects of this increased oxidative stress on cell function remain unknown. We aimed to identify changes in the metabolism of thyroid cancer cells caused by oxidative stress, using proton nuclear magnetic resonance (1H-NMR) spectroscopy. Samples of thyroid cancer and healthy thyroid tissue were collected from patients undergoing thyroidectomy and analyzed with 1H-NMR spectroscopy for a wide array of metabolites. We found a significant increase in lactate content in thyroid cancer tissue compared to healthy tissue. Metabolomic analysis demonstrated significant differences between cancer tissue and healthy tissue, including an increase in aromatic amino acids, and an average decrease in citrate in thyroid cancer tissue. We hypothesize that these changes in metabolism may be due to an oxidative stress-related decrease in activity of the Krebs cycle, and a shift towards glycolysis in cancer tissue. Thus, thyroid cancer cells are able to reprogram their metabolic activity to survive in conditions of high oxidative stress and with a compromised antioxidant system. Our findings, for the first time, suggested a connection between oxidative stress and the alteration of the metabolic profile in thyroid tumors.

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Section: Introductionsupporting

confidence: 89%