Special Issue: Molecular Properties and the Applications of Peptide Nucleic Acids

Corradini, Roberto

doi:10.3390/molecules23081977

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…As already mentioned, many attempts have been made to extend the modest half-life of conventional MBs for in vivo applications by using nanomaterial-based signalling and quenching moieties. The modification of the MB backbone or the use of DNA mimics [125] (e.g., PNA or LNA) [26,126] further contributes to overcoming limitations of traditional MBs for in vivo biosensing. The stability of PNA-DNA duplexes has been also proven to favour the incorporation of PNA into a rectangular DNA origami [127].…”

Section: Molecular Beacons and Dna Origami In Biosensing: A Comparison And Peculiar Applicationsmentioning

confidence: 99%

Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

2021

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Nucleic acid nanotechnology designs and develops synthetic nucleic acid strands to fabricate nanosized functional systems. Structural properties and the conformational polymorphism of nucleic acid sequences are inherent characteristics that make nucleic acid nanostructures attractive systems in biosensing. This review critically discusses recent advances in biosensing derived from molecular beacon and DNA origami structures. Molecular beacons belong to a conventional class of nucleic acid structures used in biosensing, whereas DNA origami nanostructures are fabricated by fully exploiting possibilities offered by nucleic acid nanotechnology. We present nucleic acid scaffolds divided into conventional hairpin molecular beacons and DNA origami, and discuss some relevant examples by focusing on peculiar aspects exploited in biosensing applications. We also critically evaluate analytical uses of the synthetic nucleic acid structures in biosensing to point out similarities and differences between traditional hairpin nucleic acid sequences and DNA origami. Graphical abstract

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Section: Molecular Beacons and Dna Origami In Biosensing: A Comparison And Peculiar Applicationsmentioning

confidence: 99%

Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

2021

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“…A promising building block for this goal is PNA, a synthetic mimic of DNA with a neutral polyamide backbone 29,30 . PNAs show structural features similar to those of DNA and have been suggested for potential applications in fields ranging from biology and chemistry to materials science [31][32][33] . Specifically, PNAs are capable of hybridizing to DNA and RNA molecules with high affinity and specificity, resulting in duplex and triplex structures 34,35 .…”

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confidence: 99%

Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid

et al. 2019

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The variety and complexity of DNA-based structures make them attractive candidates for nanotechnology, yet insufficient stability and mechanical rigidity, compared to polyamidebased molecules, limit their application. Here, we combine the advantages of polyamide materials and the structural patterns inspired by nucleic-acids to generate a mechanically rigid fluorenylmethyloxycarbonyl (Fmoc)-guanine peptide nucleic acid (PNA) conjugate with diverse morphology and photoluminescent properties. The assembly possesses a unique atomic structure, with each guanine head of one molecule hydrogen bonded to the Fmoc carbonyl tail of another molecule, generating a non-planar cyclic quartet arrangement. This structure exhibits an average stiffness of 69.6 ± 6.8 N m −1 and Young's modulus of 17.8 ± 2.5 GPa, higher than any previously reported nucleic acid derived structure. This data suggests that the unique cation-free "basket" formed by the Fmoc-G-PNA conjugate can serve as an attractive component for the design of new materials based on PNA self-assembly for nanotechnology applications.

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“…This work demonstrates the advantages of coassembly as a very useful method to expand the conformational space for the assembly of nanostructures. We believe that this study can serve as the basis for further evaluation of the unique optical properties of supramolecular self‐ and coassemblies, and could allow the expansion of the architectural diversity and physical properties, thus facilitating their optimization for various applications …”

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confidence: 99%

Coassembly of Complementary Peptide Nucleic Acid into Crystalline Structures by Microfluidics

et al. 2019

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The self‐assembly of simple units into well‐ordered supramolecular polymeric structures may give rise to a broad range of desirable attributes. In the field of bionanotechnology, there are two main classes commonly used as building blocks for self‐assembly—amino acids and nucleobases. While protein, peptide, and amino acid building blocks propose broad chemical versatility, the specific Watson–Crick base pairings of nucleic acids allow rational design and precise control over the assembly process. Specifically, artificially synthesized peptide nucleic acids (PNA), short DNA mimics that have an amide backbone, can present a unique set of properties while preserving the specific base pairing of the nucleic acids. The use of complementary building blocks allows the study of supramolecular polymer coassemblies. Here, the coassembly of two paired di‐PNA building blocks provides a new layer of control, as both building blocks are required for the spontaneous assembly to occur. Utilizing a microfluidic system, the growth of the well‐ordered structure can be regulated and monitored. The crystal structure of the formed assemblies display Watson–Crick base pairing with similar distances as those found in typical DNA double helices. Moreover, the crystals exhibit intriguing optical properties that may be implemented in various materials science and nanotechnological applications.

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Special Issue: Molecular Properties and the Applications of Peptide Nucleic Acids

Abstract: Polyamide analogs of DNA, or peptide nucleic acid (PNA), were first proposed in 1991 by a group of chemists and biochemists in a memorable Science paper [1].[…]

Cited by 3 publications

References 16 publications

Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid

Coassembly of Complementary Peptide Nucleic Acid into Crystalline Structures by Microfluidics

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