Specialized anchors for the deep sea : progress summary / by J.E. Smith, R.M. Beard, and R.J. Taylor.

HPLC-FID with Superheated Water as the Eluent: Improved Methods and Instrumentation

“…ODS columns have been reported to be unstable when used at high temperatures [13]. However, in our experiments, as well as in the experiments of Smith and Burgess [9], good results were obtained with this stationary phase. No stability problems were encountered with the ODS columns over periods of roughly a few weeks, when using these columns at temperatures below approximately 175 8C.…”

“…The observations mentioned above inspired researchers to use superheated water as an eluent for reversed phase HPLC (RP-HPLC) [8,9]. Smith and Burgess [8,9] demonstrated the separation of phenols, alkylparabens, and barbiturates applying pure water with temperatures ranging between 150 8C and 210 8C.…”

“…Smith and Burgess [8,9] demonstrated the separation of phenols, alkylparabens, and barbiturates applying pure water with temperatures ranging between 150 8C and 210 8C. A UV detector was used operating at 254 nm.…”

Examination of the signal transduction pathways leading to activation of extracellular signal‐regulated kinase by formyl‐methionyl‐leucyl‐phenylalanine in rat neutrophils

“…On the contrary, experiments using electropermeabilized human neutrophils indicated that elevation of [Ca 2 ] i is not required for activation of MEK by fMLP [41]. Our results using the PLC inhibitor U73122 [43] and the intracellular Ca 2 chelator BAPTA [44] to prevent the increase of [Ca 2 ] i showed the attenuation of fMLP-stimulated ERK phosphorylation (Fig. 5) and suggest that fMLP activates ERK also through a PLC/Ca 2 -dependent pathway.…”

Cloning, functional expression and tissue distribution of human α1C‐adrenoceptor splice variants

“…Briefly, transfected CHO cells at 50% confluence in 100-mm dishes were trypsinized and washed with BSS buffer (140 mM NaC1, 4 mM KCI, 1 mM MgCI2, 1.25 mM CaC12, 1 mM NaH2PO4, 5 mM HEPES, 11 mM glucose and 0.1% BSA, pH 7.4), and incubated in the buffer containing 4HM fura-2/AM for 30 min at 25°C. The cells were then washed twice and incubated at room temperature for 10 min in BSS with or without 10 mM of U-73122, an inhibitor for agonist induced phospholipase C activation [16]. Mobilization of [Ca2+]i evoked by 1 ruM of norepinephrine were monitored by a Jasco CAF-110 fluorescence spectrophotometer (Nihon Bunkoh, Tokyo, Japan) with dual excitation at 340 nm/ 380 nm and emission at 500 nm.…”