Speciation and antifungal susceptibility testing of candida species isolated from clinical samples

Abstract

Background: The importance of epidemiological monitoring of yeasts involved in pathogenic processes is unquestionable due to the increase in trend of infections caused by various species of Candida over the last decade; so are the changes observed in species causing Candidiasis and empirical antifungal treatment.
 Aims and Objective: To speciate the clinically isolated Candida species by phenotypic methods and to estimate the antifungal susceptibility of the isolated species against fluconazole, ketoconazole, voriconazole, itraconazole by disc diffusion method.
 Materials and Methods: A cross-sectional study conducted in 2018 in the Department of Microbiology, J. N. Medical College, KAHER, Belagavi Karnataka. Ethical Clarence was obtained from institutional ethical committee J.N. Medical College.
 Results: Out of 59 Candida isolates, Candida tropicalis was the predominant species 41(69.49%), followed by Candida glabrata 5 (8.47%), Candida parapsilosis and Candida lusitaniae 4 (6.78%) respectively, Candidaguilliermondii and Candida kefyr 2 (3.39%) respectively and the least one was Candida krusei 1 (1.69%). Voriconazole showed the highest level of sensitivity whereas Itraconazole has shown the least sensitivity pattern by disk diffusion method. Out of 59 Candida species, 52 (88.13%) were sensitive to Voriconazole, 44 (74.57%) were sensitive to Fluconazole, 40 (67.79%) were sensitive to Ketoconazole and the least sensitivity was shown by Itraconazole 30 (50.84%). Candida krusei and Candida guilliermondii showed 100% sensitive to Fluconazole, Voriconazole, Ketoconazole and Itraconazole respectively.
 Conclusion: Non-albicans Candida species are being common isolates from cases of candidiasis. Candida tropicalis is the predominant isolate, followed by Candida glabrata, Candida lusitaniae, Candida parapsilosis, Candida guilliermondii, Candida kefyr and Candida krusei. Most of the isolates were sensitive to Voriconazole.

Investigation of Vaginal Microbiota in Infertile Women Compared with Fertile and Evaluation of Correlation Between Elafin As an antimicrobial Peptide with Some Hormones

“…This result agree with the result of Othman et al, (2018) and Abdulla and Mustafa (2020) in terms of the phenotypic characteristics of the colonies All isolates also gave positive results for reaction with Gram stain, as the cells appeared oval to spherical, oval to elongated, or cylindrical in shape. This result was consistent with Singh et al,(2013) and that the appearance of Candida cells stained blue in color is a result of the Peptidoglycan layer in the cell wall retaining this dye (Sudbery et al, 2004).…”

Molecular Detection and Identification of Candida Species Isolates from Oral by RFLP-PCR

“…These characteristics obtained by the authors are similar to the characteristics of the Candida colonies when grown on the SDA medium. These results agree with many reports in terms of the appearance of creamy colonies, smooth and rounded gloss to provide appropriate culture conditions [20,21].…”

Update of the list of QPS‐recommended microbiological agents intentionally added to food or feed as notified to EFSA 16: suitability of taxonomic units notified to EFSA until March 2022

“…Singh et al. (2020) claim that 2 clinical isolates from patients with suspected candidiasis in a hospital in India were Candida kefyr . Since the species identification is very uncertain and the paper lacks information regarding any predisposing conditions in the patients, the importance of the results cannot be appropriately assessed.…”

“…Three publications contribute with information related to human safety concerns, and all three present identification problems (Singh et al., 2020; Jain et al., 2021,; Zyrek, 2021). Singh et al.…”