Speciation of inorganic selenium in environmental matrices by flow injection analysis-hydride generation-atomic fluorescence spectrometry. Comparison of off-line, pseudo on-line and on-line extraction and reduction methods

Moreno, Ma. Eva; Pérez-Conde, C.; Cámara, Carmen

doi:10.1039/a909590j

Cited by 47 publications

(19 citation statements)

References 21 publications

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“…Se concentrations in brains obtained from chickens were measured using the hydride generation-atomic fluorescence spectrometer (AFS-3200; Yongtuo Instruments) against the standard reference of Se (GBW(E)080441, National Research Center for Certified Reference Materials) [22]. Briefly, brain tissues were homogenized on ice in physiological saline and centrifuged at 1000×g and supernatants were collected for GPx, CAT, and MDA activity analysis using kits provided by Jiancheng Biotechnology Research Institute (Nanjing, China).…”

Section: Biochemical Assaysmentioning

confidence: 99%

Characterization of Selenoprotein M and Its Response to Selenium Deficiency in Chicken Brain

Huang

Ren

Jiang

et al. 2015

Biol Trace Elem Res

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Selenoprotein M (SelM) may function as thiol disulfide oxidoreductase that participates in the formation of disulfide bonds and can be implicated in calcium responses. SelM may have a functional role in catalyzing free radicals and has been associated with Alzheimer's disease (AD). However, studies of SelM in chicken remain very limited. In this study, two groups of day-old broiler chicks (n = 40/group) were fed a corn-soy basal diet (BD, 13 μg Se/kg) and BD supplemented with Se (as sodium selenite) at 0.3 mg/kg. The brain was collected at 14, 21, 28, and 42 days of age. We performed a sequence analysis and predicted the structure and function of SelM. We also investigated the effects of Se deficiency on the expression of Selt, Selw, and Selm and the Se status in the chicken brain. The results show that Se deficiency induced the lower (P < 0.05) Se content, glutathione peroxidase (GPx), and catalase (CAT) activities; increased (P < 0.05) malondialdehyde (MDA) content; and reduced (P < 0.05) the expression of Selm messenger RNA (mRNA) and protein abundance of SelM in the brain. However, there were no significant brain Selt and Selw mRNA levels by dietary Se deficiency in chicks. The different regulations of these three redox (Rdx) protein expressions by Se deficiency represent a novel finding of the present study. Our results demonstrated that SelM may have an important role in protecting against oxidative damage in the brain of chicken, which might shed light on the role of SelM in human neurodegenerative disease. More studies are needed to confirm our conclusion.

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Section: Biochemical Assaysmentioning

confidence: 99%

Characterization of Selenoprotein M and Its Response to Selenium Deficiency in Chicken Brain

Huang

Ren

Jiang

et al. 2015

Biol Trace Elem Res

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“…For real samples selenium was determined from slurry prepared as described previously [31]. The procedure for determining the amount of Se(IV) needs to switch off the coupled microwave oven in order to avoid the reduction of species.…”

Section: Operating Conditions For Volatile Generation-detectionmentioning

confidence: 99%

“…The hydrogen obtained from the volatile generation reaction and an auxiliary hydrogen flow sustained the diffusion flame in the atomisation cell. Signals were recorded using a Waters 746 integrator (Millipore, Bedford, MA, USA) and subsequently processed [31].…”

Section: Hg-aas; Hg-afs; Hg-icp-msmentioning

confidence: 99%

The effect of the presence of volatile organoselenium compounds on the determination of inorganic selenium by hydride generation

2003

Self Cite

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As a result of microbiological activity it is possible to find dimethylselenium (DMSe) and dimethyldiselenium (DMDSe) in a wide type of environmental samples, such as soils, sediments, sewage sludges and plants where methylation can take place. Selenium determination by hydride-generation (HG) techniques requires its presence as Se(IV). Consequently, inorganic speciation by hydride generation techniques is done by first determining Se(IV) and then, after reduction of Se (VI) to Se(IV), the total selenium. Therefore, the concentration of Se (VI) is evaluated as the difference between total inorganic selenium and Se(IV). In the present work it could be demonstrated that DMSe and DMDSe are forming other volatile species by reaction with sodium borohydride, applying the same reduction condition as for inorganic selenium. These species are subsequently detected by several atomic techniques (atomic absorption AAS, atomic fluorescence AFS and inductively coupled plasma-mass spectrometry ICP-MS). The error that their presence can cause in determination of inorganic selenium has been evaluated. The magnitude of this error depends on the specific analytical detector used.The coupling of pervaporation-atomic fluorescence is proposed for the identification of these species and pervaporation-gas chromatography-atomic fluorescence for their individual quantification.

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“…As noted above, selenium species must be reduced to the Se(IV) state prior to the hydride generation stage. The reduction of Se(VI) to Se(IV) by hydrochloric acid in a flow-injection manifold has been reported [20][21][22]. For organo-selenium compounds an oxidation step is required to covert the selenium to selenium (VI) prior to reduction.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of selenium by atomic fluorescence and sulfur by molecular emission by flow-injection hydride generation with on-line reduction for the determination of selenate, sulfate and sulfite

Tyson

Palmer

2009

Analytica Chimica Acta

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Simultaneous detection of selenium by atomic fluorescence and sulfur by molecular emission by flowinjection hydride generation with on-line reduction for the determination of selenate, sulfate and sulfite" (2009 An inductively coupled plasma atomic fluorescence spectrometry (ICP-AFS) instrument, was modified so that it was capable of monitoring transient chromatographic or flow-injection profiles and that sulfur molecular emission and selenium atomic fluorescence could be monitored simultaneously in an argon-hydrogen diffusion flame on a glass burner. The analytes were introduced as hydrogen selenide and hydrogen sulfide, generated on a flow-injection manifold. Selenate was reduced to hydride-forming selenite by microwave-assisted on-line reaction with hydrochloric acid, and sulfate, or sulfite, was reduced to hydride-forming sulfide by a mixture of hydriodic acid, acetic acid and sodium hypophosphite. The effects of the nature of reducing agent, flow rate, microwave power and coil length were studied. The limit of detection (3 s) for selenium was 10 g L −1 , and for sulfide was 70 g L −1 (200-L injection volume). The calibration was linear for selenium up to 2 mg L −1 and to 10 mg L −1 for sulfide. The throughput was 180 h −1 . The three sulfur species could be differentiated on the basis of reactivity at various microwave powers.

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Speciation of inorganic selenium in environmental matrices by flow injection analysis-hydride generation-atomic fluorescence spectrometry. Comparison of off-line, pseudo on-line and on-line extraction and reduction methods

Cited by 47 publications

References 21 publications

Characterization of Selenoprotein M and Its Response to Selenium Deficiency in Chicken Brain

Characterization of Selenoprotein M and Its Response to Selenium Deficiency in Chicken Brain

The effect of the presence of volatile organoselenium compounds on the determination of inorganic selenium by hydride generation

Simultaneous detection of selenium by atomic fluorescence and sulfur by molecular emission by flow-injection hydride generation with on-line reduction for the determination of selenate, sulfate and sulfite

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