Species relationship and population structure analysis in Geranium subg. Robertium with the use of ISSR molecular markers

Bozchaloyi, Somayeh Esfandani; Sheidai, Masoud; Keshavarzi, Maryam; Noormohammadi, Zahra

doi:10.1556/034.60.2018.1-2.4

Cited by 19 publications

(20 citation statements)

References 48 publications

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“…The population genetic approach is widely used for species identification and evolutionary analysis of closely related plant species (Medrano et al 2014, Stiehl-Alves et al 2017, Esfandani-Bozchaloyi et al 2018, Pinangé et al 2019. For this purpose, it is recommended to use highly polymorphic markers with large genome coverage (Duminil & Di Michele 2009) which are not influenced by environment nor affected by natural selection (Holderegger et al 2006), but experience high rate of intraspecific gene flow (Petit & Excoffier 2009).…”

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confidence: 99%

Population genetics, species boundaries, and conservation in the Magnolia pacifica species complex along a continentality and moisture gradient in western Mexico

Muñiz-Castro¹,

Castro-Félix

Carranza-Aranda³

et al. 2020

Bot. Sci.

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Background: In western Mexico, the Magnolia pacifica complex includes three morphologically defined, endemic and, endangered species, distributed along a 215 km continentality and moisture gradient: Magnolia pugana, M. pacifica s.s., and M. vallartensis. Hypothesis: Genetic evidence supports the taxonomical classification of the M. pacifica complex. Study site and dates: Western Mexico, samples were collected in 2012-2015. Methods: Six Inter-Simple Sequence Repeats (ISSR) primers amplified 76 clear and reproducible fragments in 278 individuals from 10 localities representative of the M. pacifica complex. ISSR dataset was analyzed using STRUCTURE 2.3.4, UPGMA clustering, Barrier 2.2, and AMOVA. Genetic diversity parameters were also estimated. Results: Genetic analyses revealed two main groups: M. pugana and M. pacifica s.s.–M. vallartensis. Two subpopulations within each group were evidenced, particularly M. pacifica s.s. was separated from M. vallartensis. Geographical barriers to gene flow were identified. AMOVA revealed a similar and significant proportion of variation between and within groups. M. pugana has lower genetic diversity and higher differentiation than the M. pacifica s.s-M. vallartensis group. Conclusions: Our results support the recognition of M. pugana as a genetically distinct lineage from M. pacifica s.s and M. vallartensis, but did not reveal a clear species boundary between the last two taxa. Geographical barriers and distance isolation might explain the genetic structure and differentiation pattern within the M. pacifica s.s. species complex. All main groups and subgroups defined in this study should be considered as separate conservation units, and concerted efforts are needed to protect them.

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Population genetics, species boundaries, and conservation in the Magnolia pacifica species complex along a continentality and moisture gradient in western Mexico

Muñiz-Castro¹,

Castro-Félix

Carranza-Aranda³

et al. 2020

Bot. Sci.

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“…The genus Geranium L. (Geraniaceae) with about 320 species distributed throughout the temperate regions, is chemically characterised by the presence of tannins, flavonoids, anthocyanins and essential oils which affect the extraction of pure genomic DNA (Aedo et al 1998, Esfandani-Bozchaloyi et al 2018a. There are 25 annual and perennial species of this genus in Iran according to Flora Iranica (Schönbeck-Temesy 1970).…”

Section: Introductionmentioning

confidence: 99%

Comparison of DNA extraction methods from Geranium (Geraniaceae)

Esfandani‐Bozchaloyi

Sheidai

Kalalegh

2019

Acta Botanica Hungarica

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The genus Geranium (Geraniaceae); with about 320 species throughout the temperate regions, is chemically characterised by the presence of tannins, flavonoids, anthocyanins and essential oils which interfere with the extraction of pure genomic DNA. It is necessary to optimise the extraction protocols to reduce the effects of the presence of these compounds to the lowest level. The present study compares the plant genomic DNA extraction Kit (DNP TM Kit), CTAB DNA extraction method by Murray and Thompson and Sahu et al., from the extracting DNA point of view Geranium species. The results showed significant differences in DNA contents between the three methods. Quantity and quality of extracted genomic DNAs were compared by employing the spectrophotometer, Nano-Drop, agarose gel electrophoresis, and polymerase chain reaction (PCR) methods and molecular marker such as (ITS and trnL-F) and ISSR. The method of Sahu et al., provided the best results (200 ng/µL) in terms of quantity and quality of DNA, therefore, this method was taken and optimised for DNA extraction. Our results proposed that this method could be effective for plants with same polysaccharides, proteins and polyphenols components. The advantage of this method is that it omits the use of liquid nitrogen and toxic phenols which are expensive. The success of this method in obtaining high-quality genomic DNA has been demonstrated in the Geranium species group and the reliability of this method has been discussed.

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“…They can reveal the pattern of gene flow between closely related phylogenetic species versus isolation by distance and identify the evolutionary process by which new biological species arise (Freeland et al 2011). Species delimitation is a difficult task, particularly in species with cross-pollination breeding systems that tend to form frequent inter-specific hybrids (Esfandani Bozchaloyi et al 2017b, 2018a, 2018b.…”

Section: Introductionmentioning

confidence: 99%

Molecular diversity and genetic relationships amongGeranium pusillumandG. pyrenaicumwith inter simple sequence repeat (ISSR) regions

Bozchaloyi

Sheidai

2018

Caryologia

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Successful management and preservation of natural populations depend on accurate assessment of genetic diversity. Knowing the genetic diversity within a population is important for choosing the conservation strategies for the species. Geranium pusillum L. is an annual or biennial herb mainly native to Europe, northern Africa, and western Asia, and G. pyrenaicum Burm is a frost hardy perennial native to areas of France and Spain to the Caucasus. In general, taxonomic and biosystematic studies of Geranium are not known in Iran; moreover, in a few cases, inter-specific hybrids and intermediate forms are recognized. A detailed morphological, micro-morphological and molecular (ISSR) study of Geranium is done here with the following objectives: (1) to delimitate the species; (2) to carry out a population genetic study and produce information on genetic structure and genetic variability within each population in G. pyrenaicum and G. pusillum. The present study revealed that a combination of morphological and micro-morphological (seed and pollen) data can separate the species. In the present study, 84 randomly collected plants from 12 geographical populations of two Geranium species were considered. A consensus tree based on morphological and genetic data separated some of these populations from the others, suggesting the existence of ecotypes in the studied taxa.

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Species relationship and population structure analysis in Geranium subg. Robertium with the use of ISSR molecular markers

Cited by 19 publications

References 48 publications

Population genetics, species boundaries, and conservation in the Magnolia pacifica species complex along a continentality and moisture gradient in western Mexico

Population genetics, species boundaries, and conservation in the Magnolia pacifica species complex along a continentality and moisture gradient in western Mexico

Comparison of DNA extraction methods from Geranium (Geraniaceae)

Molecular diversity and genetic relationships amongGeranium pusillumandG. pyrenaicumwith inter simple sequence repeat (ISSR) regions

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