Species Status and Relationship Between Roccella montagnei and Roccella belangeriana Using DNA Sequence Data of Nuclear Ribosomal Internal Transcribed Spacer Region

Prashanth, S. R.; Bharath, P.; Valarmathi, R.; Balaji, Petety V.; Parida, Ajay; Hariharan, G.

doi:10.1007/bf03263267

Cited by 5 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nine cases (15%) exhibit genetic uniformity relative to the studied markers or did not show structured resolution, but the available data in these cases were often limited, e.g., in Evernia esorediosa vs. E. mesomorpha (Miądlikowska et al 2014a). Considering examples such as Fuscopannaria protensa vs. F. ahlneri, Pectenia plumbea vs. P. atlantica, and the Roccella belangeriana vs. R. montagnei species pair (Prashanth et al 2008;Tehler et al 2010;Carlsen et al 2012;Otálora et al 2013;Ekman et al 2014;Marthinsen et al 2019), we predict that in cases were limited molecular data initially indicate conspecifity or would support the species pair concept, additional data will in most cases reveal more complex patterns. In ten cases (16%), the putative species pairs were, at least in part, not directly related and in one case, Caloplaca aurantiaca (= Blastenia ferruginea) vs. C. chrysophthalma (≡ Solitaria chrysophthalma), even ended up in different genera (Arup et al 2013;Vondrák et al 2020).…”

Section: The Species Pair Concept and Dual Nomenclature In Lichenized Fungimentioning

confidence: 99%

Species in lichen-forming fungi: balancing between conceptual and practical considerations, and between phenotype and phylogenomics

2021

View full text Add to dashboard Cite

Lichens are symbiotic associations resulting from interactions among fungi (primary and secondary mycobionts), algae and/or cyanobacteria (primary and secondary photobionts), and specific elements of the bacterial microbiome associated with the lichen thallus. The question of what is a species, both concerning the lichen as a whole and its main fungal component, the primary mycobiont, has faced many challenges throughout history and has reached new dimensions with the advent of molecular phylogenetics and phylogenomics. In this paper, we briefly revise the definition of lichens and the scientific and vernacular naming conventions, concluding that the scientific, Latinized name usually associated with lichens invariably refers to the primary mycobiont, whereas the vernacular name encompasses the entire lichen. Although the same lichen mycobiont may produce different phenotypes when associating with different photobionts or growing in axenic culture, this discrete variation does not warrant the application of different scientific names, but must follow the principle "one fungus = one name". Instead, broadly agreed informal designations should be used for such discrete morphologies, such as chloromorph and cyanomorph for lichens formed by the same mycobiont but with either green algae or cyanobacteria. The taxonomic recognition of species in lichen-forming fungi is not different from other fungi and conceptual and nomenclatural approaches follow the same principles. We identify a number of current challenges and provide recommendations to address these. Species delimitation in lichen-forming fungi should not be tailored to particular species concepts but instead be derived from empirical evidence, applying one or several of the following principles in what we call the LPR approach: lineage (L) coherence vs. divergence (phylogenetic component), phenotype (P) coherence vs. divergence (morphological component), and/or reproductive (R) compatibility vs. isolation (biological component). Species hypotheses can be established based on either L or P, then using either P or L (plus R) to corroborate them. The reliability of species hypotheses depends not only on the nature and number of characters but also on the context: the closer the relationship and/or similarity between species, the higher the number of characters and/or specimens that should be analyzed to provide reliable delimitations. Alpha taxonomy should follow scientific evidence and an evolutionary framework but should also offer alternative practical solutions, as long as these are scientifically defendable. Taxa that are delimited phylogenetically but not readily identifiable in the field, or are genuinely cryptic, should not be rejected due to the inaccessibility of proper tools. Instead, they can be provisionally treated as undifferentiated complexes for purposes that do not require precise determinations. The application of infraspecific (gamma) taxonomy should be restricted to cases where there is a biological rationale, i.e., lineages of a species complex that show limited phylogenetic divergence but no evidence of reproductive isolation. Gamma taxonomy should not be used to denote discrete phenotypical variation or ecotypes not warranting the distinction at species level. We revise the species pair concept in lichen-forming fungi, which recognizes sexually and asexually reproducing morphs with the same underlying phenotype as different species. We conclude that in most cases this concept does not hold, but the actual situation is complex and not necessarily correlated with reproductive strategy. In cases where no molecular data are available or where single or multi-marker approaches do not provide resolution, we recommend maintaining species pairs until molecular or phylogenomic data are available. This recommendation is based on the example of the species pair Usnea aurantiacoatra vs. U. antarctica, which can only be resolved with phylogenomic approaches, such as microsatellites or RADseq. Overall, we consider that species delimitation in lichen-forming fungi has advanced dramatically over the past three decades, resulting in a solid framework, but that empirical evidence is still missing for many taxa. Therefore, while phylogenomic approaches focusing on particular examples will be increasingly employed to resolve difficult species complexes, broad screening using single barcoding markers will aid in placing as many taxa as possible into a molecular matrix. We provide a practical protocol how to assess and formally treat taxonomic novelties. While this paper focuses on lichen fungi, many of the aspects discussed herein apply generally to fungal taxonomy. The new combination Arthonia minor (Lücking) Lücking comb. et stat. nov. (Bas.: Arthonia cyanea f. minor Lücking) is proposed.

show abstract

Section: The Species Pair Concept and Dual Nomenclature In Lichenized Fungimentioning

confidence: 99%

Species in lichen-forming fungi: balancing between conceptual and practical considerations, and between phenotype and phylogenomics

2021

View full text Add to dashboard Cite

show abstract

“…These two taxa are considered as synonyms by Tehler et al (2010), although they could not integrate the holotype into their phylogenetic analysis of Roccella species from the Paleotropics (because they were unable to receive the holotype on loan). Prashanth et al (2008) also asserted that R. montagnei and R. belangeriana are the same species, basing their conclusion on the analysis of ITS sequences and indicating that these two taxa are similar in both morphology and secondary chemistry. The results obtained here tend to reinforce the existence of at least two taxa in the R. montagnei-belangeriana complex.…”

Section: R Montagnei-belangeriana Complexmentioning

confidence: 95%

“…The phylogenetically close species R. montagnei and R. belangeriana (Tehler 2007;Prashanth 2008) were further studied. Using the HPLC-MS data of both species, a PCA analysis was performed for dimension reduction of multivariate data whilst preserving most of the variance for both species.…”

Section: Focus On the R Montagnei-belangeriana Complexmentioning

confidence: 99%

Chemical diversity of five coastal Roccella species from mainland France, the Scattered Islands, and São Tomé and Príncipe

Ferron¹,

Berry²,

Olivier-Jimenez³

et al. 2020

Plant and Fungal Systematics

View full text Add to dashboard Cite

Roccella species constitute interesting models to address questions regarding lichen metabolite diversity across taxonomic, ecological and geographic gradients. Indeed, owing to their wide distribution, their taxonomic diversity and the narrow ecological niche they occupy, Roccella species are good candidates to study the drivers of lichen chemistry. This study focuses on the chemical profiling of five species: R. applanata, R. belangeriana, R. fuciformis, R. montagnei and R. phycopsis. These five species were sampled in a rather narrow longitudinal range (1°51′W to 47°17′E) covering the Eastern Atlantic and Western Indian Ocean areas along an extended latitudinal range (48°49′N to 22°23′S). High Pressure Liquid Chromatography (HPLC) analysis followed by mass spectrometry of 31 Roccella thalli revealed a number of interesting patterns through a multivariate (PCA) analysis, including the first detailed chemical profiles for two species from the Scattered Islands: R. applanata and R. belangeriana. Metabolite segregation amongst all studied Roccella species, including R. montagnei and R. belangeriana, gave some insight into the taxonomy of the latter two species, which we interpret as separate species. An additional analysis focusing on R. montagnei samples revealed chemical differences along both a latitudinal and ecological gradient (from Europa Island to São Tomé and Príncipe). Three mass spectra databases were built to dereplicate the ions, which gave an overview of the factors that could drive quantitative and qualitative metabolite composition in lichens. Additionally, several new Roccella species records are reported for the Scattered Islands, as well as São Tomé and Príncipe.

show abstract

“…Rocclella genus distributes in the subtropical regions, Mediterranean and mainly in the Northern hemisphere 1 . Roccella montagnei species is commonly found along the Coromandel coast and in the mangrove forest in Indian and in the South of Vietnam 2 .…”

Section: Introductionmentioning

confidence: 99%

The phytochemical investigation from n-hexane extract of the lichen Roccella montagnei

Nguyen

Huy

2021

Sci. Tech. Dev. J.

View full text Add to dashboard Cite

Introduction: R occella montagnei is widely distributed in subtropical regions. As the continuous study on the hexane extract of Roccella montagnei lichen, the isolation and structural determination of five compounds were addressed. Method: The crude extract was obtained from the dried lichen powder's extraction at room temperature. The n-hexane, n-hexane-ethyl acetate, and ethyl acetate extracts were obtained by the liquid-liquid partition method. The organic compounds were isolated from n-hexane extract by silica gel and Sephadex LH-20 column chromatography. Their chemical structures were identified by the NMR and HR-ESI-MS data analysis and the comparison of their NMR data with the published data. Results: Five compounds were isolated and chemically structural identified, consisting of 3b -hydroxy-7a-methoxystigmast-5-ene (1), sekikaic acid (2), lichenxanthone (3), (+)-6,8-dihydroxy-3-propyl-3,4-dihydroisocoumarin (4), and ar-turmerone (5). Conclusion: To the best of our knowledge, except 3 which was reported from this species for the first time, four isolated compounds left did not known to be present in Roccella genus before.

show abstract

Species Status and Relationship Between Roccella montagnei and Roccella belangeriana Using DNA Sequence Data of Nuclear Ribosomal Internal Transcribed Spacer Region

Cited by 5 publications

References 6 publications

Species in lichen-forming fungi: balancing between conceptual and practical considerations, and between phenotype and phylogenomics

Species in lichen-forming fungi: balancing between conceptual and practical considerations, and between phenotype and phylogenomics

Chemical diversity of five coastal Roccella species from mainland France, the Scattered Islands, and São Tomé and Príncipe

The phytochemical investigation from n-hexane extract of the lichen Roccella montagnei

Contact Info

Product

Resources

About