Specific activation of the DNA-synthesizing apparatus in contact-inhibited mouse kidney cells by polyoma virus.

Kára, J.; Weil, Roger

doi:10.1073/pnas.57.1.63

Cited by 53 publications

(14 citation statements)

References 16 publications

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“…Although the number of RNA polymerase II molecules was found to remain constant during infection with SV40 (9), studies with inhibitors of protein and RNA synthesis suggest the de novo formation of enzymes involved in DNA replication (3,4,5). However these studies need to be expanded before a quantitative arguement for the de novo synthesis of DNA polymerases can be made.…”

Section: Diseussionmentioning

confidence: 98%

“…The rise in activity of the DNA polymerases upon infection with SV40 appears to originate from modifications in cellular metabolism (1,4,5), such as the regulation of mRNA transcription, control of translation at the ribosomal level (28), modifications in the cellular membrane with the appearance of a viral-coded tumor antigen (29) and a change in the glyeotipid composition to resemble that found in cancerous tissue (30,31). Modifications in the cell membrane could readily effect the calcium ion balance which plays a pivotal role in.…”

Section: Diseussionmentioning

confidence: 99%

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Fluctuation in activity of the molecular forms of cellular DNA polymerase during infection by SV40

Lazarus

Kitron

1976

Archives of Virology

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Infection of BSC-1 cells by SV40 brings about an increase of 7--11-fold in DNA polymerase activity, found in the nuclei and cytoplasm, respectively. The overall ratio between activites of DNA polymerase beta (3.1S) and DNA polymerase alpha (5.5S) remains fairly constant throughout infection. However,there is a large increase in DNA polymerase alpha2 (7.1S) in the cytoplasm, and its appearance in the nuclei late in infection. The addition of 1 M NaCl to infected cytoplasm,causes an aggregation of DNA polymerase alpha into a higher sedimenting form (9.8S), termed DNA polymerase alpha3. DNA polymerase alpha1, alpha2 and alpha3 are different molecular forms of the same enzyme, as can be seen by their similar inhibition by N-ethyl-maleimide, heparin and NaCl. However, this new activity, alpha3, is stimulated by dithiothreitol to a greater extent at pH 9.30 than at pH 7.94. The conformational changes induced in DNA polymerase and its increase in activity during infection with SV40 are discussed.

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Section: Diseussionmentioning

confidence: 98%

Section: Diseussionmentioning

confidence: 99%

Fluctuation in activity of the molecular forms of cellular DNA polymerase during infection by SV40

Lazarus

Kitron

1976

Archives of Virology

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“…Fifty ~l of the cell extract were employed for determination of protein concentration by the method of LOWRY (8). Thymidine kinase was measured as described by K~A and WEIL (9). The enzyme activity was expressed in ~ moles dTMP 2-C 14 formed in 30 minutes per 1 mg of protein.…”

Section: Effect Of Temperature On Synthesis Of T and V Antigens Of Pamentioning

confidence: 99%

Effect of temperature on synthesis of T and V antigens of papovavirus SV40 and induction of thymidine kinase

Vonka¹,

Kára²,

Kutinovà³

et al. 1969

Archiv f Virusforschung

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The syntheses of tumor (T) and viral capsid (V) antigens of papovavirus SV40 proceed differently at different temperatures. Using complement fixation (CF), I~T~A and 35EL~ICX (1) found that the peak T antigen titers were the same at 23 ~ as at 37~ although the first appearance of the T antigen was significantly delayed at the lower temperature. At 23~ the V antigen synthesis was markedly suppressed and the period between the appearance of detectable levels of the T and the V antigens was prolonged. The thermal separation of synthesis of T and V antigens was then applied to adenoviruses (2).Infection of monkey kidney cells with SV40 results in early synthesis not only of T antigen, but also of thymidine kinase. Their synthesis is not inhibited by drugs that prevent DNA synthesis (3, 4). However, KIT et al. (5) clearly demonstrated the non-identity of T antigen and thymidine kinase.In an attempt to separate the synthesis of T antigen and thymidine kinase, we studied their development in green monkey kidney (GMK) cells infected with SV40 and incubated at subnormal temperature. About 2 • 10 ~ GM_K cells in 5 ml of Parker's medium 199 supplemented with 2~o calf serum were seeded in 60 mm plastic Petri dishes, which were then incubated at 37~ in an atmosphere with 5% CO S. Some of the Petri dishes contained coverslips. Twenty-four hours after confluent cell sheets had been formed, the cultures were infected with SV40 (Baylor strain) at an input multiplicity of about 10 TCDs0 per cell. After 2 hours incubation

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“…the cell cycle (8), as are most resting mammalian cells (5).. Whether Investigations of intracellular changes following release of contact inhibition had been conducted on cells released from contact inhibition by reseeding cells in a larger growth area after trypsinization, by adding fresh serum (9,10) or by transforming these cells (11)(12)(13) Serum stimulation of division in contact inhibited cells does not cause physical cell separation, and biochemical alterations seen after addition of fresh serum to a confluent culture might be due to stimuli other than those operating in an authentic release of contact inhibition.…”

Section: A Laboratory Historical Backgroundmentioning

confidence: 99%

Molecular events basic to cellular radiation response. Progress report

Kolodny¹

1974

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Specific activation of the DNA-synthesizing apparatus in contact-inhibited mouse kidney cells by polyoma virus.

Cited by 53 publications

References 16 publications

Fluctuation in activity of the molecular forms of cellular DNA polymerase during infection by SV40

Fluctuation in activity of the molecular forms of cellular DNA polymerase during infection by SV40

Effect of temperature on synthesis of T and V antigens of papovavirus SV40 and induction of thymidine kinase

Molecular events basic to cellular radiation response. Progress report

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