Specific amplification of a DNA sequence common to all Chlamydia trachomatis serovars using the polymerase chain reaction

Dutilh, B; Bébéar, Christiane; Rodríguez, Patricia; Vekris, Antoine; Bonnet, Jacques; Garret, Maurice

doi:10.1016/0923-2508(89)90053-3

Cited by 101 publications

(36 citation statements)

References 14 publications

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“…These include the chlamydial cryptic plasmid (pCT) (43,97,134,163,164), omp1, coding for MOMP (27,63,96,134,163), the gene coding for 16S rRNA (2,43,134), and omp2, coding for OmcB (239). With the exception of pCT, all of these targets are sequences found on the C. trachomatis chromosome, which includes two complete rRNA operons and single copies of omp1 and omp2.…”

Section: Direct Hybridization Probe Testsmentioning

confidence: 99%

Diagnosis and Assessment of Trachoma

et al. 2004

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Trachoma is caused by Chlamydia trachomatis. Clinical grading with the WHO simplified system can be highly repeatable provided graders are adequately trained and standardized. At the community level, rapid assessments are useful for confirming the absence of trachoma but do not determine the magnitude of the problem in communities where trachoma is present. New rapid assessment protocols incorporating techniques for obtaining representative population samples (without census preparation) may give better estimates of the prevalence of clinical trachoma. Clinical findings do not necessarily indicate the presence or absence of C. trachomatis infection, particularly as disease prevalence falls. The prevalence of ocular C. trachomatis infection (at the community level) is important because it is infection that is targeted when antibiotics are distributed in trachoma control campaigns. Methods to estimate infection prevalence are required. While culture is a sensitive test for the presence of viable organisms and nucleic acid amplification tests are sensitive and specific tools for the presence of chlamydial nucleic acids, the commercial assays presently available are all too expensive, too complex, or too unreliable for use in national programs. There is an urgent need for a rapid, reliable test for C. trachomatis to assist in measuring progress towards the elimination of trachoma

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Section: Direct Hybridization Probe Testsmentioning

confidence: 99%

Diagnosis and Assessment of Trachoma

et al. 2004

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“…Recently, the polymerase chain reaction (PCR) has been applied to the detection and differentiation of the three Chlamydia species by using primers specific for the MOMP gene (Dutilh et al, 1989;Holland et al, 1990). Furthermore, preliminary communications have reported intra-species characterization of C .…”

Section: Introductionmentioning

confidence: 99%

Restriction pattern of the major outer-membrane protein gene provides evidence for a homogeneous invasive group among ruminant isolates of Chlamydia psittaci

Denamur

Sayada

Souriau

et al. 1991

Journal of General Microbiology

105

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Thirty-six ruminant isolates of Chlamydia psittaci, previously classified as invasive or non-invasive in a mouse model of virulence, were compared by analysing A h 1 restriction patterns of the major outer-membrane protein (MOMP) gene after DNA amplification by the polymerase chain reaction. The 24 invasive isolates, although from various origins, all belonged to serotype 1 and represented a strictly homogeneous group sharing a specific MOMP-gene restriction'pattern that was not observed in the non-invasive strains. On the other hand, the 12 noninvasive strains, although all belonging to serotype 2, constituted a heterogeneous group with eight distinct MOMP-gene restriction patterns. However, all eight patterns shared a 180 bp fragment or the corresponding restricted fragments of 110 and 70 bp. MOMP-gene restriction patterns also clearly distinguished the ruminant strains from an avian C.psi#uciisolate, a Cpneumoniae isolate and two C. truchomatis isolates which were studied for comparison. The homogeneous character of the invasive C. psiffuci strains argues strongly for their genetic relatedness. Our results illustrate the usefulness of the MOMP-gene restriction mapping in typing chlamydiae.

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“…However, this procedure is laborious and at best no more than 75% sensitive 5 . Currently available alternative tests include enzyme immunoassays (EIAs), direct uorescent antibody (DFA), nucleic acid probes 6 and deoxyribonucleic acid (DNA) ampli® cation methods such as polymerase chain reaction (PCR) 7 and the ligase chain reaction (LCR) 8 . The commonest test used in genitourinary medicine (GUM) clinics in the UK is EIA/ enzyme-linked immunosorbent assay 3 .…”

Section: Introductionmentioning

confidence: 99%

Screening for genital chlamydia infection: DNA amplification techniques should be the test of choice

2003

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Our objective was to compare the sensitivities for the detection of Chlamydia trachomatis, of the ligase chain reaction (LCR) on first voided urine (FVU) specimens and enzyme immunoassay (EIA) on pooled endocervical/endourethral swabs from women and endourethral swabs from men. Men and women taking part in the UK chlamydia screening pilot were tested for chlamydia using LCR on a FVU. Patients attending genitourinary medicine clinics also had cervical and/or urethral swabs taken for chlamydia testing by EIA. In women, EIA on pooled swabs detected 575 of the 785 chlamydia positives and in men, EIA detected 209 of 351 positives. The sensitivity of EIA was 73% and 60% in women and men respectively. By using the EIA test, therefore, 27-40% of patients infected with chlamydia will be given a false negative result. We propose that it is unethical to use non-molecular testing in the future.

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Specific amplification of a DNA sequence common to all Chlamydia trachomatis serovars using the polymerase chain reaction

Cited by 101 publications

References 14 publications

Diagnosis and Assessment of Trachoma

Diagnosis and Assessment of Trachoma

Restriction pattern of the major outer-membrane protein gene provides evidence for a homogeneous invasive group among ruminant isolates of Chlamydia psittaci

Screening for genital chlamydia infection: DNA amplification techniques should be the test of choice

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