Specific and instantaneous one-step chemodetection of histidine-rich proteins by Pauly's stain

Sahal, Dinkar; Kannan, R; Sinha, Amritanshu; Babbarwal, Vinod; Prakash, Bhanu; Singh, Gajinder Pal; Chauhan, V. S.

doi:10.1016/s0003-2697(02)00210-5

Cited by 17 publications

(22 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1b). Some of the protein bands were positive for Pauly's stain, indicating the presence of His-rich polypeptides 21 . In addition, nitroblue tetrazolium (NBT) staining showed that the smaller proteins contained catecholic residues, whereas a lectin-binding assay confirmed the presence of chitin oligomers in the highermolecular-weight fractions.…”

Section: Gigas Chitin-binding Beak Proteinsmentioning

confidence: 99%

“…Proteins were separated by gel electrophoresis by Tricine-SDS-PAGE using 4% stacking and 16% resolving gels, prepared as previously described 46 , and then stained either with Coomassie Blue R-250 (CBR) stain 46 , Pauly's stain 21 or nitroblue tetrazolium (NBT) stain 47 , which stain for generic proteins, His-rich and catecholic proteins, respectively. Approximately 10 μg of proteins were loaded in each lane for CBR stain and 30 μg for Pauly's stain and NBT, unless otherwise stated.…”

Section: Gel Electrophoresismentioning

confidence: 99%

See 1 more Smart Citation

Infiltration of chitin by protein coacervates defines the squid beak mechanical gradient

et al. 2015

View full text Add to dashboard Cite

Section: Gigas Chitin-binding Beak Proteinsmentioning

confidence: 99%

Section: Gel Electrophoresismentioning

confidence: 99%

Infiltration of chitin by protein coacervates defines the squid beak mechanical gradient

et al. 2015

View full text Add to dashboard Cite

“…[39] Property contour maps were generated using the Origin graphing software (OriginLab, Northampton, MA, USA). Pauly's solution [40] , which specifically stains for His-rich proteins. Prior to Pauly's staining, gels were rinsed in isopropanol and water in order to remove the residual SDS, a prerequisite for the staining technique.…”

Section: Microscopy (Sem) and Energy Dispersive Spectroscopy (Eds) Amentioning

confidence: 99%

Microstructural and Biochemical Characterization of the Nanoporous Sucker Rings from Dosidicus gigas

et al. 2009

View full text Add to dashboard Cite

show abstract

“…A total of 4 µg of each AGP fraction was dot‐blotted onto a 0.22 µm nitrocellulose membrane (Osmonics Inc., Westborough, MA) and then air‐dried. Pauly's stain was applied to the membrane following the methods of Sahal et al (2002) with the following changes: 1% sulfanilic acid (Sigma‐Aldrich) and 5% sodium nitrate were chilled and an equal volume of both solutions was mixed while still cold. The mixture was sprayed lightly, without forming pools of stain, onto the membrane which was pre‐wet with MilliQ water.…”

Section: Methodsmentioning

confidence: 99%

Investigation of a His‐rich arabinogalactan‐protein for micronutrient biofortification of cereal grain

Aizat

Preuss

Johnson

et al. 2011

Physiologia Plantarum

View full text Add to dashboard Cite

The micronutrient content of most cereal grains is low and responsible for malnutrition deficiencies in millions of people who rely on grains as their primary food source. Any strategy that can increase the micronutrient content of grain will have significant benefits to world health. We identified a gene from barley encoding a cell wall protein with multiple histidine (His)-rich motifs interspersed with short arabinogalactan-protein (AGP) domains and have called it Hordeum vulgare His-rich AGP (HvHRA1). Sequence analysis shows that His-rich AGPs are rare in plants and that the number of His-rich and AGP domains differ between cereals and dicots. The barley and wheat encoded proteins have more than 13 His-rich domains, whereas the putative rice orthologue has only 5 His-rich regions. His-rich motifs are well-established metal-binding motifs; therefore, we developed transgenic (Tx) rice plants that constitutively overexpress barley HvHRA1. There was no significant effect on plant growth or grain yield in Tx plants. Purification of AGPs from wild-type and Tx plants showed that only Tx plants contained detectable levels of a His-rich AGP. Calcein assay shows that the AGP fraction from Tx plants had increased binding affinity for Cu(2+) . Micronutrient analysis of brown and white rice showed that the grain nutrient yield for Fe, Zn and Cu was higher in two Tx lines compared to their respective nulls, although the differences were not statistically significant. This approach highlights the potential of the plant apoplast (cell wall) for storage of key nutrients through overexpression of genes for metal-binding proteins.

show abstract

Specific and instantaneous one-step chemodetection of histidine-rich proteins by Pauly's stain

Cited by 17 publications

References 12 publications

Infiltration of chitin by protein coacervates defines the squid beak mechanical gradient

Infiltration of chitin by protein coacervates defines the squid beak mechanical gradient

Microstructural and Biochemical Characterization of the Nanoporous Sucker Rings from Dosidicus gigas

Investigation of a His‐rich arabinogalactan‐protein for micronutrient biofortification of cereal grain

Contact Info

Product

Resources

About