Specific and targeted detection of viable Escherichia coli O157:H7 using a sensitive and reusable impedance biosensor with dose and time response studies

Dweik, Majed; Stringer, R. Cody; Dastider, Shibajyoti Ghosh; Wu, Yifan; Almasri, Mahmoud; Barizuddin, Syed

doi:10.1016/j.talanta.2012.02.056

Cited by 51 publications

(30 citation statements)

References 35 publications

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“…The LoD was found to be as low as 3.5·10 1 CFU· mL -1 , which is comparable with or better than other detection limit values previously reported for direct label-free bacterial detection (Basu et al, 2014;Dweik et al, 2012;EscamillaGómez et al, 2009;Li et al, 2014;Silva et al, 2014;Varshney and Li, 2007).…”

Section: Sensitivity Of the System In Kcl Solutionsupporting

confidence: 85%

Impedimetric antimicrobial peptide-based sensor for the early detection of periodontopathogenic bacteria

Hoyos‐Nogués

Brosel-Oliu

Abramova

et al. 2016

Biosensors and Bioelectronics

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Peri-implantitis, an inflammation caused by biofilm formation, constitutes a major cause of implant failure in dentistry. Thus, the detection of bacteria at the early steps of biofilm growth represents a powerful strategy to prevent implant-related infections. In this regard, antimicrobial peptides (AMPs) can be used as effective biological recognition elements to selectively detect the presence of bacteria. Thus, the aim of the present study was to combine the use of miniaturized and integrated impedimetric transducers and AMPs to obtain biosensors with high sensitivity to monitor bacterial colonization. Streptococcus sanguinis, which is one of the most prevalent strains in the onset of periodontal diseases, was used as a model of oral bacteria. To this end, a potent AMP derived from human lactoferrin was synthesized and covalently immobilized on interdigitated electrode arrays (IDEA). X-ray photoelectron spectroscopy (XPS) and electrochemical impedance spectroscopy (EIS) were employed to optimize and characterize the method of immobilization. Noteworthy, the interaction of Streptococcus sanguinis with AMP-coated sensors provoked significant changes in the impedance spectra, which were univocally associated with the presence of bacteria, proving the feasibility of our method. In this regard, the developed biosensor permits to detect the presence of bacteria at concentrations starting from 10(1) colony forming units (CFU)mL(-1) in KCl and from 10(2) CFUmL(-1) in artificial saliva. Moreover, the system was devoid of cytotoxicity for human fibroblasts. These results indicate that the proposed approach can be effective in the detection of initial stages of biofilm formation, and may be useful in the early prevention and treatment of peri-implantitis.

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Section: Sensitivity Of the System In Kcl Solutionsupporting

confidence: 85%

Impedimetric antimicrobial peptide-based sensor for the early detection of periodontopathogenic bacteria

Hoyos‐Nogués

Brosel-Oliu

Abramova

et al. 2016

Biosensors and Bioelectronics

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“…Ruminants, especially bovines, are the principal reservoir of STEC, and human contamination is often associated with consumption ground beef and direct contact with the animals or their environment (Savoye et al, 2011) STEC strains are highly pathogenic to human in low infectious doses, causing food-borne diseases through consumption of contaminated water or food (Dweik et al, 2012). Bovine milk and other dairy products such as yogurt and cheese have been associated with diseases caused by STEC (Chapman et al, 1993;Prandel et al, 2008;Martin & Beutin, 2011).…”

Section: Introductionmentioning

confidence: 99%

Molecular screening of bovine raw milk for the presence of Shiga toxin-producing Escherichia coli (STEC) on dairy farms

Vendramin¹,

Kich²,

Molina³

et al. 2014

Food Sci. Technol (Campinas)

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“…9. At low frequency (<1 MHz), the Cdi acting as an open circuit (Dweik et al 2012), so the function of impedance (Z) can be expressed as following (5):…”

Section: Debugging Working Parameters and Optimization Of The Self-asmentioning

confidence: 99%

“…He et al (He et al 2013) used electrophoresis technology on a microfluidic chip to enrich for bacteria, realizing the effective real-time on-line detection of Salmonella typhimurium. Interdigital microelectrodes have routinely been used for dielectrophoresis enrichment and impedance detection on microfluidic chips (Dweik et al 2012). Their advantages include sensitive signal responses, rapid achievement of stable state and a large detection area that could improve detection sensitivity.…”

Section: Introductionmentioning

confidence: 99%

An integrated microsystem with dielectrophoresis enrichment and impedance detection for detection of Escherichia coli

Wang

Liu

et al. 2017

Biomed Microdevices

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An integrated microsystem device with matched interdigitated microelectrode chip was fabricated for enrichment and detection of Escherichia coli O157:H7. The microsystem has integrated with positive dielectrophoresis (pDEP) enrichment and in situ impedance detection, whose total volume is only 3.0 × 10 m, and could provide impedance testing voltages of 0 ~ 10 V, detection frequencies of 1 KHz ~ 1 MHz, DEP excitation signals with amplitude of 0 ~ 10 Vpp and frequencies of 1KHz ~ 1 MHz, which fully meets the demands of pDEP enrichment and impedance detection for bacteria. The microfluidic chip with interdigitated microelectrodes was manufactured by microfabrication methods. The interdigital microelectrode array has sufficient contact area with a bacterial suspension to improve enrichment efficiency and detection sensitivity. Bacteria in the interdigital microelectrode area of the microfluidic chip were firstly captured and enriched by pDEP. Then, in situ impedance detection of the enriched bacteria was realized by switching test conditions. Using the self-assembly microsystem, a novel quantitative detection method was established and demonstrated to detect Escherichia coli O157:H7. Experimental results showed that the detection limits of Escherichia coli O157:H7 was 5 × 10 cfu mL, and testing time was only 6 min under the optimized detection voltage of 100 mV and frequency of 500 KHz. The method was successfully used to detect Escherichia coli O157:H7 in synthetic chicken synthetic samples.

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Specific and targeted detection of viable Escherichia coli O157:H7 using a sensitive and reusable impedance biosensor with dose and time response studies

Cited by 51 publications

References 35 publications

Impedimetric antimicrobial peptide-based sensor for the early detection of periodontopathogenic bacteria

Impedimetric antimicrobial peptide-based sensor for the early detection of periodontopathogenic bacteria

Molecular screening of bovine raw milk for the presence of Shiga toxin-producing Escherichia coli (STEC) on dairy farms

An integrated microsystem with dielectrophoresis enrichment and impedance detection for detection of Escherichia coli

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