1992
DOI: 10.1016/0021-9673(92)80139-l
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Specific assay for endotoxin using immobilized histidine, Limulus amoebocyte lysate and a chromogenic substrate

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Cited by 11 publications
(9 citation statements)
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“…In the adsorption method, the LPS concentration in various compounds could be accurately determined even at a low concentration of LPS (0.1 -0.5 EU mL -1 ), without sample dilution. Minobe et al reported similar data for a specific LPS assay using histidine-immobilized sepharose as adsorbent, 6,7 but the recovery was low at ionic strength (μ) ≥0.1. This was due to the low adsorption of LPS on the adsorbent because of the high ionic strength of the solution containing LPS.…”
Section: Assay Of Lps Using Pl-cellufinementioning
confidence: 73%
See 1 more Smart Citation
“…In the adsorption method, the LPS concentration in various compounds could be accurately determined even at a low concentration of LPS (0.1 -0.5 EU mL -1 ), without sample dilution. Minobe et al reported similar data for a specific LPS assay using histidine-immobilized sepharose as adsorbent, 6,7 but the recovery was low at ionic strength (μ) ≥0.1. This was due to the low adsorption of LPS on the adsorbent because of the high ionic strength of the solution containing LPS.…”
Section: Assay Of Lps Using Pl-cellufinementioning
confidence: 73%
“…The samples were dissolved in one of the following buffers: 0.02 M sodium acetate (pH 4, 5), 0.02 M phosphate (pH 6,7,8), and 0.02 M Tris (pH 9), and the pH and ionic strength were adjusted. The ionic strength of the buffer was adjusted by changing the sodium chloride concentration.…”
Section: Selective Adsorption Of Lps On Pl-cellufinementioning
confidence: 99%
“…After Separation, the activity of the endotoxins adsorbed on immobilized histidine can then be directly assayed by the Limulus amoeboeyte lysate test without any Inhibition or enhancement. This procedure had a high reproducibility and accuracy, and had certäin advantages for the determination of endotoxins in a solution containing Limulus amoeboeyte lysate-inhibiting or -enhancing substances, compared with the more common gel-clot technique (3,4).…”
Section: Immobilized Histidine Adsorbs Endbtoxins In Solutions Contaimentioning
confidence: 99%
“…We reported the use of this immobilized histidine method for the determination of endotoxin in industrial and pharmaceutical samples (3,4). However, it is more difficult to determine endotoxin in plasma samples than in industrial or pharmaceutical samples, and endotoxin assay in plasma is important for tlie diagnosis of Gramnegative sepsis.…”
Section: Immobilized Histidine Adsorbs Endbtoxins In Solutions Contaimentioning
confidence: 99%
“…In the dilution method, LPS sensitivity decreases because the LPS concentration is also diluted. Minobe et al attempted to develop a new specific assay method for LPS using histidineimmobilized sepharose as an adsorbent [6,7]. Adsorbed LPS by the adsorbent is separated from the compound (e.q., amino acids, enzymes) by centrifugation, and the LPS adsorbed on the adsorbent can reacted directly with the LAL.…”
Section: Introductionmentioning
confidence: 99%