Specific cell-mediated cytotoxicity against an allogeneic target cell line in isogeneic ginbuna crucian carp

In vivo Priming of TNP-reactive Effector Cells in Carp.

“…Furthermore, in mice that were immunized with TNP-conjugated synge neic spleen cells on the 2nd day after cyclophosphamide treatment, maximal TNP-specific cytolysis of the lymph node cells was observed 4 days after in vivo immuniza tion (Rollinghoff et al, 1977). Additionally, recent re ports in fish described that specific cytotoxicity against allogeneic antigen could be induced by using cell lines (Hasegawa et al, 1998)and erythrocytes (Fischer et al, 1998). Graft-versus-host reaction (GVHR) also showed similar induction (Nakanishi and Ototake 1999).…”

“…Cell-mediated cyto toxic activity, in particular nonspecific cell-mediated cytotoxic activity has been described in fish (Evans and Jaso-Friedmann, 1992;Yoshinaga et al, 1994;Kurata et al, 1995). On the other hand, to our knowledge, spe cific-cell mediated cytotoxicity in fish have been studied only in xenogeneic responses (Sakai, 1984), allogeneic responses (Hashimoto and Ikeda, 1987;Stuge et al, 1997;Hasegawa et al, 1998;Fischer et al, 1998;Nakanishi and Ototake 1999), and in vitro autologous responses (Verlhac et al, 1990).…”

Intraovarian Cavity Leucocytes of Viviparous Fish, Neoditrema ransonneti (Perciformes, Embiotocidae)

“…Macrophages in pregnant human uterus are major sites of production of prostaglandin E 2 (Norwits et al ., 1991), which suppresses lymphocyte activation and proliferation in placenta Lala et al ., 1988;Parhar et al ., 1989;Kvirkvelia et al ., 2002). Though cytotoxic T cells and natural killer cells have not been identified distinctively in fish, it has been demonstrated that cytotoxic cells recognizing allogenicity also exist (Yoshida et al ., 1995;Stuge et al ., 1995;Fisher et al ., 1998;1999;Hasegawa et al ., 1998;1999). In the viviparous poeciliid fish, Xiphophorus hellerii , the fertilization membrane which surrounds fetuses during gestation may protect them from the maternal rejection (Hogarth, 1973).…”

Modulation of MHC class II expression in rainbow troutOncorhynchus mykissmacrophages by TNFα and LPS

“…with specific helper and cytotoxic activity are known in fish (Clem et al, 1991;Hasegawa et al, 1998), and potential CD4/CD8 binding domains have been identified in the MHC sequences (Stet et al, 1996;Rode, 1997), it is likely that MHC restricted T cell subpopulations will be present. In mammals, a critical step in both specific humoral and cell-mediated immune responses is the release of cytokines from helper T cells, which itself is dependent upon appropriate presentation of peptides by MHC class II molecules on macrophages, dendritic cells and B cells (Rohn et al, 1996).…”