Specific lipopolysaccharides of <i>Bordetella</i>

Maclennan, A. P.

doi:10.1042/bj0740398

Cited by 62 publications

(28 citation statements)

References 35 publications

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“…Isolation of lipopolysaccharide ( LPS) LPS was extracted from the two strains by Westphal's hot phenol-water method according to MacLennan (10), and treated further with DNase, RNase, and trypsin.…”

Section: Methodsmentioning

confidence: 99%

Polymorphonuclear Leukocyte‐Inhibitory Factor of Bordetella pertussis

Imagawa

Kanoh

Sonoda

et al. 1980

Microbiology and Immunology

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In vivo biologic effects of the polymorphonuclear leukocyte-inhibitory factor (PIF) of Bordetella pertussis were tested by using two experimentally induced. inflammatory processes in mice. The intravenous injection of a partially purified extract from phase I bacteria strongly inhibited the glycogen-induced peritoneal infiltration of polymorphonuclear leukocytes (PMN) and the Arthus reactions, whereas little inhibitory activity was found in the extract from phase III bacteria. The activity was localized in the outer membrane of phase I bacteria, as was the in vitro PIF activity, and the two activities gave the same behavior in DEAE-cellulose chromatography.Therefore the observed suppression of inflammatory processes in mice is probably due to the inhibitory action of PIF on the function of PMN in vivo.Earlier studies have shown that Bordetella pertussis vaccine renders its recipients more susceptible to a variety of bacterial (1, 3, 5, 6) and viral (13) infections. These organisms might produce an active component which depresses the host's defense mechanism. Thus Levine and Sowinsky (9) made an interesting observation that injection of pertussis vaccine inhibited the migration of monocytes into thermally coagulated, necrotized loci in the brains of experimental mice. The polymorphonuclear leukocyte-inhibitory factor (PIF), which has been shown to be produced by virulent strains of B. pertussis and to interfere with the phagocytic and chemotactic functions of human polymorphonuclear leukocytes (PMN) in vitro (4,14), may also be related to the deterioration of the host's defense. Therefore we designed experiments to see if PIF could also block the PMN-mediated inflammatory responses in vivo by the use of the experimental systems of Arthus reactions and glycogen-induced peritoneal infiltration of PMN in the mouse. The results indicate that this may indeed be the case.

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Section: Methodsmentioning

confidence: 99%

Polymorphonuclear Leukocyte‐Inhibitory Factor of Bordetella pertussis

Imagawa

Kanoh

Sonoda

et al. 1980

Microbiology and Immunology

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“…Serum antibodies to B. bronchiseptica were detected by an indirect haemagglutination test using goose erythrocytes sensitized with specific B. bronchiseptica lipopolysaccharide (MacLennan, 1960).…”

Section: Serologymentioning

confidence: 99%

A study of chronic pneumonia in a guineapig colony with enzootic Bordetella bronchiseptica infection

Baskerville

Wood³

1982

Lab Anim

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Three types of lesion were present in the lungs of guinea pigs with chronic pneumonia - pulmonary interstitial fibrosis, perivascular lymphoid hyperplasia and foreign body granulomas. Bordetella bronchiseptica was present in the nasopharynx, trachea and lungs of a high proportion of animals from 4 weeks old, and antibodies to this organism were also found. Animals removed at 4 days old and reared in isolation from the main colony and free from B. bronchiseptica developed similar lung lesions, so that B. bronchiseptica appeared to have no causative role in these forms of chronic pneumonia.

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“…It has been noted by MacLennan [5] that endotoxins isolated from members of the genus Bordetella are unusually resistant to acid-catalysed hydrolysis. This has been confirmed both in the previous [7] and the present study, treatment with quite strong acid being required (4 M HC1, 100 "C, 2 h) to release the uronic acid present.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, it has been established that uronic acids are integral parts of the endotoxins of some gramnegative bacteria which do not belong to the family Enterobucteriuceae. Thus Volk [4] demonstrated that D-galacturonic acid was present in the endotoxin of 17 different species of Xunthomonus (Pseudomonuduceue) and MacLennan observed [5] that during acidic hydrolysis of the endotoxin extracted from Bordetellu bronchiseptica (Brucelluceue) carbon dioxide was released in amounts indicating the presence of about 20% of uronic acid in that lipopolysaccharide. It was, however, stressed by MacLennan that the presence of uronic acids could not be demonstrated either by paper chromatography of the hydrolysate or by colorimetric methods.…”

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confidence: 99%