Specific recognition of hydatid cyst antigens by serum IgG, IgA using Western blot

Sbihi, Younes; Janssen, Dirk; Osuna, Antonio

doi:10.1002/(sici)1098-2825(1997)11:3<154::aid-jcla6>3.0.co;2-a

Cited by 14 publications

(13 citation statements)

References 21 publications

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“…2). The immunoblotting pattern was same as that of previous studies [14-16]. The present EcAg which we used in this study was confirmed as the same strain of sheep E. granulosus by the immunoblotting pattern.…”

Section: Discussionsupporting

confidence: 86%

Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep

Jin

Anvarov²,

Khajibaev³

et al. 2013

Korean J Parasitol

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According to increase of travel, the cases of imported echinococcosis have been increasing in Korea. The present study was undertaken to develop a serodiagnostic system for echinococcosis in Korea. For diagnosis of echinococcosis, the fluid of Echinococcus granulosus hydatid cysts was collected from naturally infected sheep in Uzbekistan. Also serum samples of infected patients who were surgically confirmed were collected in a hospital in Tashkent, Uzbekistan. According to the absorbance of 59 echinococcosis positive and 39 negative control serum samples, the cut-off value was determined as 0.27. The sensitivity and specificity of ELISA with hydatid fluid antigen were 91.5% and 96%, respectively. The antigen cross-reacted with the serum of some cysticercosis or clonorchiasis patients. However, immunoblot analysis on the cystic fluid recognized antigenic proteins of 7-, 16-, and 24-kDa bands in their dominant protein quantity and strong blotting reactivity. In conclusion, the present ELISA system using hydatid cyst fluid antigen from Uzbekistan sheep is sensitive and specific for diagnosis of echinococcosis cases.

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Section: Discussionsupporting

confidence: 86%

Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep

Jin

Anvarov²,

Khajibaev³

et al. 2013

Korean J Parasitol

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“…Our ELISA results are similar to those obtained by Zarzosa et al (1999). In our WB, we could not get sensitivity or specificity results higher than 90 % for single bands, despite the high sensitivity and specificity results obtained with antigen 5 or antigen B by others (Kanwar et al, 1992;Sbihi et al, 1997). The discrepancy may be attributed to the differences in using purified antigen or electrophoresis conditions.…”

Section: Discussionsupporting

confidence: 56%

“…According to the ROC curve analysis, the band of 120-124 kDa is observed as the most important band for differentiation. There aren't enough studies about high molecular masses in CE patients (Sbihi et al, 1997;Kanwar et al, 1992;Shambesh et al, 1995). In a previous study, we frequently found a band of 124 kDa in patients with pulmonary CE (Akisu et al, 2005).…”

mentioning

confidence: 99%

Comparative evaluation of Western Blotting in hepatic and pulmonary cystic echinococcosis

Akısü

Delıbaş

Biçmen³

et al. 2006

Parasite

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Summary :Many serological tests are widely used in the diagnosis of cystic echinococcosis (CE), caused by the larval stages of Echinococcus granulosus. The present study was carried for differentiation between hepatic and pulmonary cystic echinococcosis by Western Blotting (WB). A total of 121 sera from patients with hepatic CE (37), pulmonary CE (31) and controls (53; consisting of six healthy, seven Hymenolepis nana infection, 20 hepatic and 20 pulmonary diseases other than CE) were examined. In all of the CE patients, E. granulosus infection was confirmed by surgical intervention. Sera were previously tested using IHA and ELISA to detect the E. granulosus specific antibodies. Sera from hepatic cases of CE reacted with 16 polypeptides of 6-116 kDa and sera from pulmonary cases of CE reacted with 14 polypeptides of 4-130 kDa by Western Blotting. The WB test enabled the detection of antibodies in the hepatic CE samples for proteins of 24, 32-34, 44-46 and 52-54 kDa in molecular weight in 78.4 %, 75.7 %, 78.4 % and 89.2 % of the patients, respectively. In the pulmonary CE samples sera WB test enabled the detection of antibodies 24, 44-46, 100, 110, 116 and 120-124 kDa in molecular weight in 81.3 %, 75.0 %, 87.5 %, 71.9 %, 84.4 % and 65.6 % of the patients, respectively. We indicated that the antigenic components of high molecular weight can be good candidates for differentiation of hepatic CE from pulmonary CE. de 24,[32][33][34][44][45][46]4 %, 75,7 %, 78,4 % et 89,2 % des patients, respectivement, et, en cas de KH pulmonaire, de protéines de 24,[44][45][46] 100, 110,[116][117][118][119][120][121][122][123][124]3 %, 75,0 %, 87,5 %, 71,9 %, 84,4 % et 65,6 Résumé

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“…The levels of additional antibody isotypes, such as IgE, IgA, and IgM, seem to decline 2 to 4 years after the cure, as reported elsewhere (2,4,8,21). Unfortunately, only a limited number of UHD patients show detectable levels of these isotype antibodies (21).…”

Section: Vol 15 2008 Serodiagnosis and Follow-up Of Hydatid Diseasementioning

confidence: 99%

Usefulness of Four Different Echinococcus granulosus Recombinant Antigens for Serodiagnosis of Unilocular Hydatid Disease (UHD) and Postsurgical Follow-Up of Patients Treated for UHD

Hernández-González

Muro

Barrera

et al. 2008

Clin Vaccine Immunol

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Four different recombinant antigens derived from Echinococcus granulosus, designated B1t, B2t, E14t, and C317, were tested with enzyme-linked immunosorbent assays (ELISAs) for the detection of specific immunoglobulin G (IgG) in patients with unilocular hydatid disease (UHD). The results were compared to those obtained with hydatid fluid and were subjected to receiver operator characteristic analysis. The diagnostic performance of the above-listed proteins was defined with respect to their specificity, sensitivity, and predictive values (PV); the influence of cyst location; and usefulness in the follow-up of surgical treatment for UHD and in the determination of whether or not patients have been surgically cured of UHD. The best diagnostic results were obtained with the anti-B2t IgG ELISA, with 91.2% sensitivity, 93% specificity, and high positive and negative PV (89.4 and 94.2, respectively). In addition, this diagnostic tool proved to be useful for the follow-up of surgically treated UHD patients. The anti-B2t IgG ELISA may find an application in the serodiagnosis of UHD in clinical laboratories.Human unilocular hydatid disease (UHD) is caused by the larvae of the tapeworm Echinococcus granulosus. The diagnosis of UHD usually is approached by applying an imaging technique. In 2003, the World Health Organization Informal Working Group on Echinococcosis (28) proposed the use of ultrasonography (US) as the imaging technique of choice in order to promote uniform standards of UHD diagnosis and follow-up. It also was pointed out that US images suspected of showing UHD should be examined by alternative diagnostic methods, such as serological techniques (28). In addition, for pulmonary UHD, US is unhelpful in most cases, unless the cysts are close to the pleural surface (12). Currently, the preferred UHD immunodiagnostic techniques used in clinical practice detect serum immunoglobulin G (IgG) against crude parasite extracts (mainly hydatid fluid [HF]), most commonly by hemagglutination and enzyme-linked immunosorbent assay (ELISA) (26). However, HF cannot be standardized and gives rise to relatively frequent false-positive and false-negative results (3, 15). Moreover, HF is not useful for the follow-up of UHD, since the levels of specific (IgG) antibodies against HF remain high over long periods of time after curing (19). Many authors have attempted to find alternative antigens to improve UHD serodiagnosis and follow-up. Thus, purified, recombinant antigens and synthetic peptides, mainly derived from two parasite molecules (antigen B [AgB] and antigen 5 [Ag5]), have been applied, with various levels of success. Specifically, antigens derived from AgB have been shown to be good candidates for UHD serodiagnosis (3). AgB is formed by several subunits (6), two of which (AgB1 and AgB2) have been produced as recombinant molecules. Both AgB1 and AgB2 have been tested for the detection of specific antibodies, although authors disagree about the diagnostic performance of these two subunits (11,20,27). Thus, the standardization ...

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Specific recognition of hydatid cyst antigens by serum IgG, IgA using Western blot

Cited by 14 publications

References 21 publications

Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep

Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep

Comparative evaluation of Western Blotting in hepatic and pulmonary cystic echinococcosis

Usefulness of Four Different Echinococcus granulosus Recombinant Antigens for Serodiagnosis of Unilocular Hydatid Disease (UHD) and Postsurgical Follow-Up of Patients Treated for UHD

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