Specific recognition to create nanofunctionalized cells for precise functions

Zhang, Tong-Kai; Geng, Wei; Huang, Yao-Qi; Wang, Fa-Zhou; Tian, Ge; Yang, Xiao-Yu

doi:10.1016/j.ccr.2023.215471

Cited by 4 publications

(2 citation statements)

References 163 publications

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“…In recent years, considerable effort has been devoted to overcoming this limitation . One approach, the emerging cell surface engineering technology, has been utilized to encapsulate cells with nanomaterials to enhance the efficiency of hydrogen production. − For example, Tang et al found that silicification-induced green algae aggregates achieve sustainable photobiological hydrogen production under natural aerobic conditions . Huang et al strategically utilized encapsulation of cells in an oxygen-consuming shell comprised of polydopamine, laccase, and tannic acid to enhance their hydrogen production efficiency .…”

Section: Introductionmentioning

confidence: 99%

External Electrons Directly Stimulate Escherichia coli for Enhancing Biological Hydrogen Production

Zhou,

Zhang,

Geng

et al. 2024

ACS Nano

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External electric field has the potential to influence metabolic processes such as biological hydrogen production in microorganisms. Based on this concept, we designed and constructed an electroactive hybrid system for microbial biohydrogen production under an electric field comprised of polydopamine (PDA)-modified Escherichia coli (E. coli) and Ni foam (NF). In this system, electrons generated from NF directly migrate into E. coli cells to promote highly efficient biocatalytic hydrogen production. Compared to that generated in the absence of electric field stimulation, biohydrogen production by the PDA-modified E. coli-based system is significantly enhanced. This investigation has demonstrated the mechanism for electron transfer in a biohybrid system and gives insight into precise basis for the enhancement of hydrogen production by using the multifield coupling technology.

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Section: Introductionmentioning

confidence: 99%

External Electrons Directly Stimulate Escherichia coli for Enhancing Biological Hydrogen Production

Zhou,

Zhang,

Geng

et al. 2024

ACS Nano

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“…Glycans are also attractive targets for modifications. − Researchers explored using N-glycans to create antibody–drug conjugates (ADCs). Zhu et al employed galactosidase to generate a uniform G0F glycan and then derivatized the N-acetylglucosamine to link the cytotoxic drug auristatin F to the mAb.…”

Section: Introductionmentioning

confidence: 99%

In-Membrane Enrichment and Peptic Digestion to Facilitate Analysis of Monoclonal Antibody Glycosylation

Yang,

Ostafe,

Bruening

2024

Anal. Chem.

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The number of therapeutic monoclonal antibodies (mAbs) is growing rapidly due to their widespread use for treating various diseases and health conditions. Assessing the glycosylation profile of mAbs during production is essential to ensuring their safety and efficacy. This research aims to rapidly isolate and digest mAbs for liquid chromatography−tandem mass spectrometry (LC−MS/MS) identification of glycans and monitoring of glycosylation patterns, potentially during manufacturing. Immobilization of an Fc region-specific ligand, oFc20, in a porous membrane enables the enrichment of mAbs from cell culture supernatant and efficient elution with an acidic solution. Subsequent digestion of the mAb eluate occurred in a pepsin-modified membrane within 5 min. The procedure does not require alkylation and desalting, greatly shortening the sample preparation time. Subsequent LC−MS/MS analysis identified 11 major mAb N-glycan proteoforms and assessed the relative peak areas of the glycosylated peptides. This approach is suitable for the glycosylation profiling of various human IgG mAbs, including biosimilars and different IgG subclasses. The total time required for this workflow is less than 2 h, whereas the conventional enzymatic release and labeling of glycans can take much longer. Thus, the integrated membranes are suitable for facilitating the analysis of mAb glycosylation patterns.

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Increase of the degradation activity of Phanerochaete chrysosporium by hybridization with silica nanoparticles

Li,

Geng,

et al. 2024

Journal of Physics and Chemistry of Solids

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Specific recognition to create nanofunctionalized cells for precise functions

Cited by 4 publications

References 163 publications

External Electrons Directly Stimulate Escherichia coli for Enhancing Biological Hydrogen Production

External Electrons Directly Stimulate Escherichia coli for Enhancing Biological Hydrogen Production

In-Membrane Enrichment and Peptic Digestion to Facilitate Analysis of Monoclonal Antibody Glycosylation

Increase of the degradation activity of Phanerochaete chrysosporium by hybridization with silica nanoparticles

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