Specific Selection of Host Cell Glycoproteins during Assembly of Murine Leukaemia Virus and Vesicular Stomatitis Virus: Presence of Thy-1 Glycoprotein and Absence of H-2, Pgp-1 and T-200 Glycoproteins on the Envelopes of these Virus Particles

Calafat, Jero; Janssen, Hans; Démant, Peter; Hilgers, J.; Závada, Jan

doi:10.1099/0022-1317-64-6-1241

Cited by 49 publications

(32 citation statements)

References 14 publications

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“…Glycosylphosphatidylinositol (GPI)-linked proteins such as CD55, PLAP and Thy-1 (Calafat et al, 1983;Harder et al, 1998;Marschang et al, 1995) are the most abundant Cold Triton X-100 treatment solubilizes the bulk phase of the plasma membrane, but the raft domains remain insoluble. The raft fraction floats upon centrifugation from the bottom of a sucrose gradient, whereas cytoplasmic and dissolved plasma membrane proteins remain at the bottom of the tube.…”

Section: The Raft Passengersmentioning

confidence: 99%

“…Pseudotyping of VSV has been observed with togaviruses, retroviruses, bunyaviruses, arenaviruses, paramyxoviruses, orthomyxoviruses, coronaviruses, herpesviruses and poxviruses (Altstein et al, 1976;Calafat et al, 1983;Dragunova & Závada, 1979;Lukashevich & Závada, 1982;Pastorekova et al, 1992;Schnitzer et al, 1977;Zajac et al, 1980;Závada, 1972b;Závada et al, 1972Závada et al, , 1978Závada et al, , 1983Zavadova & Závada, 1980). CD4 is incorporated efficiently (Schnell et al, 1996).…”

Section: Vesicular Stomatitis Virusmentioning

confidence: 99%

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Do lipid rafts mediate virus assembly and pseudotyping?

Briggs

Wilk

Fuller

2003

Journal of General Virology

114

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Section: The Raft Passengersmentioning

confidence: 99%

Section: Vesicular Stomatitis Virusmentioning

confidence: 99%

Do lipid rafts mediate virus assembly and pseudotyping?

Briggs

Wilk

Fuller

2003

Journal of General Virology

114

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“…A long-standing observation related to pseudotyping has been that molecules from the host cell can be selectively incorporated into viral particles (4,10,34,35,41,63,71,81,84). The list of such molecules is extensive, but an important finding has been that many of the molecules that have been found to be incorporated into one virus have also been found in others.…”

mentioning

confidence: 99%

“…The list of such molecules is extensive, but an important finding has been that many of the molecules that have been found to be incorporated into one virus have also been found in others. Glycosyl phosphatidylinositol (GPI)-anchored proteins are recurrently represented in the list (10,41,63), and many such proteins have been found to localize to plasma membrane lipid microdomains (7,11). For example the raft-localized (58) CD4 molecule (67,68,71) and the associated human immunodeficiency virus (HIV) coreceptor CXCR4 (68,71) can be incorporated into viral envelopes at a level high enough to allow targeting of HIV-infected cells.…”

mentioning

confidence: 99%

Lipid Rafts and Pseudotyping

Pickl

Pimentel‐Muiños

Seed

2001

J Virol

154

168

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Specific interactions between envelope and core proteins govern the membrane assembly of most enveloped viruses. Despite this, mixed infections lead to pseudotyping, the association of the viral cores of one virus with the envelopes of another. How does this occur? We show here that the detergent-insoluble lipid rafts of the plasma membrane function as a natural meeting point for the transmembrane and core components of a phylogenetically diverse collection of enveloped viruses. As a result, viral particles preferentially incorporate both the envelope components of other viruses as well as the extra-and intracellular constituents of host cell lipid rafts, including gangliosides, glycosyl phosphatidylinositol-anchored surface proteins, and intracellular signal transduction molecules. Pharmacological disruption of lipid rafts interferes with virus production.

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“…for HLA-DR but not HLA-DP and HLA-DQ using flow cytometry (Schols et al, 1992), and for HLA-class I and II and fl2-microglobulin, but not 11 other cell surface components, using ELISA of solubilized virions (Hoxie et al, 1987). Selectivity in the presence of certain hostderived molecules on vesicular stomatitis virus (VSV) and murine leukemia virus (MLV) (described below) has also been described (Calafat et al, 1983). This discrepancy can be ascribed to differences in the methods applied.…”

mentioning

confidence: 99%

Host cell membrane proteins on human immunodeficiency virus type 1 after in vitro infection of H9 cells and blood mononuclear cells. An immuno-electron microscopic study

Meerloo¹,

Sheikh²,

Bloem³

et al. 1993

Journal of General Virology

100

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Human immunodeficiency virus type 1 (HIV-1)-infected H9 and blood mononuclear cells (MNCs) were studied by immunogold electron microscopy for the presence of HIV-1 gag p24 protein, env gp41 and gpl20 proteins, and host cell molecules CD4, CDlla, CD25, CD54, CD63, HLA class I and HLA-DR. Uninfected H9 cells and MNC membranes labelled for CD4, HLA class I and class II, and, at low density, CDlla and CD54; lysosomal structures in the cytoplasm labelled for CD63. The infected cell surface showed immunolabelling for HIV-1 proteins, as did budding particle-like structures.Immunogold labelling of the cell membrane for CD4 was almost non-existent. The level of immunolabelling for CD1 la and CD54 on infected cells was greater than that on uninfected cells; this is presumably related to a state of activation during virus synthesis. Budding particle-like structures and free virions in the intercellular space were immunogold-labelled for all host cell markers investigated. This was confirmed by double immunogold labelling using combinations of HIV-1 gag p24 labelling and labelling for the respective host cell molecule. We conclude that virions generated in HIV-1-infected cells concentrate host-derived molecules on their envelope. Also molecules with a prime function in cellular adhesion concentrate on the virion.Infection of cells by human immunodeficiency virus type 1 (HIV-1) is followed by the disappearance of the virus receptor molecule CD4 from the cell membrane (Geleziunas et al., 1991 ;Gielen et al., 1989; Hoxie et al., 1986). This phenomenon has also been observed for other surface molecules including HLA antigens (Eales et al., 1988; Gelderblom et al., 1987b;Henderson et al., 1987;Kerkau et al., 1989;Schols et al., 1992) and the CD3, CD8 and CDll antigens (Stevenson et al., 1987). By using immuno-electron microscopy we have previously demonstrated the complete absence of CD4 antigen and the partial absence of HLA-DR and CD5 antigen on H9 cells 2 days after HIV-1 infection (Meerloo et al., 1992). The CD3 and CD25 antigens remained detectable on the cell surface at similar density, and the CD63 antigen, a t Present address:

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Specific Selection of Host Cell Glycoproteins during Assembly of Murine Leukaemia Virus and Vesicular Stomatitis Virus: Presence of Thy-1 Glycoprotein and Absence of H-2, Pgp-1 and T-200 Glycoproteins on the Envelopes of these Virus Particles

Cited by 49 publications

References 14 publications

Do lipid rafts mediate virus assembly and pseudotyping?

Do lipid rafts mediate virus assembly and pseudotyping?

Lipid Rafts and Pseudotyping

Host cell membrane proteins on human immunodeficiency virus type 1 after in vitro infection of H9 cells and blood mononuclear cells. An immuno-electron microscopic study

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