Specificity of a mycoplasma mitogen for lymphocytes from human and various animal hosts

Cole, Barry C.; Washburn, Leigh Rice; Sullivan, G J; Ward, John

doi:10.1128/iai.36.2.662-666.1982

Cited by 28 publications

(9 citation statements)

References 14 publications

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“…For example, spleen cells of mice possessing the haplotypes H-2'' or H-2'' were reactive, whereas mice of the haplotypes H-2'', H-2f, H-21, and H-2' were non-reactive. MAS is also capable of stimulating human peripheral lymphocytes and those of rat spleens [6]. In the present study we have investigated several inbred rat strains to test whether there was a similar dependency of the MAS response on the MHC, the RTI system [9], as previously reported in mice.…”

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confidence: 54%

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Lymphoproliferative Responses of Spleen Cells of Inbred Rat Strains to Mycoplasma arthritidis Mitogen

et al. 1984

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A potent mitogen for T lymphocytes of various species has recently been isolated from the supernatant of cultured Mycoplasma arthritidis organisms (MAS). In the mouse, reactivity to this mitogen has been observed to be controlled by the I-E subregion of the major histocompatibility complex. We have analysed the responses of spleen cells from several inbred rat strains covering practically all known haplotypes of the major histocompatibility complex of the rat (RT1). Unlike in the mouse, all of these responded well to MAS, except for the BN rat strain, which is a low responder to all T-cell mitogens, including phytohaemagglutinin and concanavalin A. This unresponsiveness, however, appeared to be unrelated to the RT1 haplotype, since LEW.1N rats carrying the same RT1n haplotype as BN animals responded well. Mice of the strain C57BL/6 are non-responders to MAS, but--as previously shown--their spleen cell responses can be reconstituted by the addition of 2-mercaptoethanol. No such reconstitution was observed for the low responsiveness of BN rat spleen cells. Stimulation with MAS induced high titres of interferon (presumably gamma interferon) in spleen cells from all rat strains tested. Spleen cells from BN rats produced lower interferon activities than those from other strains.

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confidence: 54%

“…The recent studies of Cole et al [6] showed MAS to be a potent mitogen for lymphocytes of several species, whereas lymphocytes of some other species were non-responsive. Responding species included humans, rats, and mice.…”

Section: Discussionmentioning

confidence: 99%

Lymphoproliferative Responses of Spleen Cells of Inbred Rat Strains to Mycoplasma arthritidis Mitogen

et al. 1984

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“…To determine whether rats, which have an overall V , genomic composition almost identical to that of mice (29), respond to MAM in a similar manner, we assessed the responses of spleen cells from a strain of rats known to be a high responder to this mitogen (Lewis rats) (10,30). As indicated in Figure 1, transcript levels for rat V , (rV& 5.1, 6, 8.1, and 8.2 were strongly enriched in these cultures but rVp8.3, in contrast to mV,8.3, was not engaged.…”

Section: Resultsmentioning

confidence: 99%

Mycoplasma arthritidis mitogen. V_β engaged in mice, rats, and humans, and requirement of HLA‐DRα for presentation

Baccalà

Smith

Vestberg

et al. 1992

Arthritis & Rheumatism

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Objective. Mycoplasma arthritidis mitogen (MAM) is mitogenic for mouse, rat, and human T cells, and behaves as a superantigen in mice through its capacity to bind to the (Y chain of I-E molecules and engage entire sets of T cells expressing specific Vp. Here, we have attempted to fully characterize the V,-engaging activities of MAM in mice, and define similar activities in rats and humans.Methods. Multiprobe RNase-protection assays and mice transgenic for human DRcu, DRP, and DRaP were utilized for this purpose.Results. MAM-reactive V ' in the mouse included not only the previously reported V&, Vp8.1, Vp8.2, and V9.3, but also V,5.1. In the rat, engagement of Vs.1, V&, V9.1, and V'8.2, but not Vp8.3, was documented, whereas in humans, the engaged V , included primarily V,19.1 (alternatively termed V'17.1) and, to a lesser ~.. Publication No. 7007-1MM from the

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“…Besides, mycoplasma-infected tumor cell lines have been reported to produce a higher incidence of metastasis (Stackpole et al 1988), as well as an increased invasiveness (Dudler et al 1988). Furthennore, difTerent mycoplasmas have been recognized to be polyclonal mitogens for both B and/or T celts from various species in an MHC-restricted or -unrestricted way; the mitogenic effects are host-specific and more or less cell type-specific (Cole et al 1982, Naot et al 1977. It has also been demonstrated that mycoplasmas can induce Ig secretion from B lymphocytes, as well as secretion of various cytokines from several cell types.…”

Section: Mycoplasmas and The Immune Systemmentioning

confidence: 99%

“…Various mycoplasma strains including M. fermentans, M. pneumoniae, and M. arthritidis have been discovered to be polycional activators for T lymphocytes of several species including mice, rat and human (Naol et al 1977, Cole et al 1982; human T cells are activated by all three strains. Besides acting as a polyclonal activator in vivo, M. pulmonis has been shown to be mitogenic in vitro for both rat and mice T and B cells (Naot et al 1979a); membrane proteins are the essential component of this activity, whereas isolated membrane lipids are devoid of any mitogenicity (Naot et al 1979b).…”

Section: Mycoplasma Effects On T Lymphocytes In Vitromentioning

confidence: 99%

Interactions between Mycoplasmas and the Immune System

Ruuth

Praz

1989

Immunological Reviews

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Mycoplasmas are a heterogenous group of prokaryotic organisms causing a wide variety of diseases, including autoimmune disorders. Thus, it is not surprising that various mycoplasmas strains, including Mycoplasma arginini, M. arthritidis, M. neurolyticum and M. pulmonis, are able to regulate the immune response. Though some of the studies of the immunomodulatory action of mycoplasmas have been done in vivo, the majority of the investigations have been conducted in vitro. This has led to the recognition that mycoplasmas are polyclonal activators of both B and T cells from several species, acting through MHC-restricted or -unrestricted pathways. Mycoplasma activation not only induces T-cell proliferation but also leads but to the formation of cytotoxic T cells. We, as well as others, have shown that mycoplasma-mediated B-cell activation induces proliferation as well as Ig secretion, and also that mycoplasma stimulation of lymphocytes may result in the production of cytokines. We communicate here our investigations into the effects of an M. arginini strain on the growth and maturation of preactivated B cells. After an initial biological characterization of the M. arginini effects in vitro, we established the protein nature of the growth-supporting activity and proceeded further on to isolate and identify the responsible proteins. The use of lipid- and lipoglycan-free extracts has allowed us to further extend our studies on the biological activities of the proteins from M. arginini and to compare these results with the effects obtained using live organisms. Furthermore, the study was extended to include a characterization of the in vivo-induced effects of live M. arginini. Altogether, the results from these experiments allow us to conclude that M. arginini is a T-cell independent polyclonal B-cell mitogen, mediated by five identified proteins, inducing growth and Ig secretion of both resting and preactivated B cells.

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Specificity of a mycoplasma mitogen for lymphocytes from human and various animal hosts

Cited by 28 publications

References 14 publications

Lymphoproliferative Responses of Spleen Cells of Inbred Rat Strains to Mycoplasma arthritidis Mitogen

Lymphoproliferative Responses of Spleen Cells of Inbred Rat Strains to Mycoplasma arthritidis Mitogen

Mycoplasma arthritidis mitogen. V_β engaged in mice, rats, and humans, and requirement of HLA‐DRα for presentation

Interactions between Mycoplasmas and the Immune System

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Specificity of a mycoplasma mitogen for lymphocytes from human and various animal hosts

Cited by 28 publications

References 14 publications

Lymphoproliferative Responses of Spleen Cells of Inbred Rat Strains to Mycoplasma arthritidis Mitogen

Lymphoproliferative Responses of Spleen Cells of Inbred Rat Strains to Mycoplasma arthritidis Mitogen

Mycoplasma arthritidis mitogen. Vβ engaged in mice, rats, and humans, and requirement of HLA‐DRα for presentation

Interactions between Mycoplasmas and the Immune System

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Mycoplasma arthritidis mitogen. V_β engaged in mice, rats, and humans, and requirement of HLA‐DRα for presentation