Specificity of Incomplete " Cold " Antibody in Human Serum

Crawford, Hal; Cutbush, Marie; Mollison, P. L.

doi:10.1016/s0140-6736(53)91694-8

Cited by 35 publications

(9 citation statements)

References 4 publications

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“…Such cells also react with anti-C5 but EC produced as described above by the low ionic strength method Dacie (1950), and its specificity, detailed by Crawford, Cutbush, and Mollison (1953), has often been used to make cells coated with C4 alone.…”

Section: Cells Sensitized With Antibodiesmentioning

confidence: 99%

Preparation of test cells for the antiglobulin test

Stratton

Rawlinson

1974

J Clin Pathol

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Erythrocytes may be coated with blood group antibodies with or without reacting complement or sometimes apparently with complement alone. This may occurin vivoin such conditions as autoimmune acquired haemolytic anaemia, haemolytic disease of the newborn, or after transfusions of incompatible blood. It may occurin vitroalso by the deliberate sensitization of erythrocytes during laboratory serological investigations.Blood group antibodies may be of immunoglobulin types γM, γA, or γG; we have never seen γD antibodies. The presence of these antibodies on the erythrocyte surface, together with complement components or the presence of complement components alone, may be detected by the direct antiglobulin test where sensitization occursin vivoor by the indirect antiglobulin test where there is sensitizationin vitro.

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Section: Cells Sensitized With Antibodiesmentioning

confidence: 99%

Preparation of test cells for the antiglobulin test

Stratton

Rawlinson

1974

J Clin Pathol

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“…It would not attach to the patient's cells at body temperature in the presence of complement nor would it attach to the patient's cells at lower temperature in the presence of comple ment, except as an agglutinin, for when such sensitized cells were heated again to 37° C, the antigen-antibody aggregate dissociated. In this patient's serum, however, the normal cold incomplete anti-H antibody [2,1] appeared to be increased in titre and in the tempera ture at which it was active. Tests showed that this antibody was active at as high a temperature as 16° C, which is unusual, although the tests were actually done at 9° C (Table II).…”

Section: Discussionmentioning

confidence: 53%

Haemoglobinuria Following Blood Transfusion

Stratton¹,

Bailey²

1963

Vox Sanguinis

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“…He regarded the presence of this substance as able to protect the foetus from the action of Rh antibodies, but it is unlikely that this hypothesis is true, because it is generally accepted that in all cases where a mother has antibodies an Rh-positive baby will have haemolytic disease, if one accepts a positive Coombs test on cord blood as defining the limits of this condition. Moreover, Crawford, Cutbush, and Mollison (1953) found that A " secretors " are not protected from ABO-haemolytic disease. In our series liquor was obtained from th.ee immunized Rh-negative mothers.…”

Section: Discussionmentioning

confidence: 99%