Spectral-domain phase microscopy

Choma, Michael A.; Ellerbee, Audrey K.; Yang, Changhuei; Creazzo, Tony L.; Izatt, Joseph A.

doi:10.1364/ol.30.001162

Cited by 331 publications

(200 citation statements)

References 7 publications

Supporting

Mentioning

195

Contrasting

Order By: Relevance

“…The technique is based on spectral-domain optical coherence tomography 8,9 to produce depth-resolved intensity and phase profiles with significantly improved phase stability compared with those of time-domain OCT based systems. [10][11][12] Because SD-OCPM acquires depth-resolved information without mechanical scanning of the reference mirror, it can generate a threedimensional quantitative phase-contrast image of a specimen simply by scanning the beam laterally as it measures phase profiles in depth. In this Letter we present, as an initial demonstration, the application of SD-OCPM to two-dimensional quantitative phasecontrast imaging in reflection.…”

mentioning

confidence: 99%

“…The lateral resolution was measured as 2.6 m (FWHM). In our method the reflection from the top surface of a coverslip serves as the reference optical field, 8,12 whereas the backscattered waves from the sample are the measurement fields [ Fig. 1(b)].…”

mentioning

confidence: 99%

“…The phase sensitivity can be characterized by the variance (or the standard deviation) of the phase, and it is expressed as an explicit function of the signal-tonoise ratio (SNR) as ͗⌬ 2 ͘Ϸ1/͑2SNR͒. 12,13 To quantify the phase sensitivity of SD-OCPM we measured the phase related to the interference of the top and bottom surfaces of a coverslip. Figure 2 shows a histogram based on the measurement performed for ϳ21 s. The measured SNR was 100.4 dB, under which condition the theoretical sensitivity is 0.4 pm.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Spectral-domain optical coherence phase microscopy for quantitative phase-contrast imaging

et al. 2005

View full text Add to dashboard Cite

We describe a novel microscopy technique for quantitative phase-contrast imaging of a transparent specimen. The technique is based on depth-resolved phase information provided by common path spectraldomain optical coherence tomography and can measure minute phase variations caused by changes in refractive index and thickness inside the specimen. We demonstrate subnanometer level path-length sensitivity and present images obtained on reflection from a known phase object and human epithelial cheek cells.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Spectral-domain optical coherence phase microscopy for quantitative phase-contrast imaging

et al. 2005

View full text Add to dashboard Cite

show abstract

“…One of the limits of 3D deconvolution is that it tends to reduce the sensitivity by the introduction of noise [11] and because of the required high phase stability it could not be applied to in vivo imaging [10]. SD-OCT has been applied to cellular imaging [2][3][4][5] and also offers the benefit of increased phase stability [12][13][14][15].…”

Section: Biophotonicsmentioning

confidence: 99%

Artefact reduction for cell migration visualization using spectral domain optical coherence tomography

Höfer¹,

Povazˇay²,

Hermann³

et al. 2011

Journal of Biophotonics

View full text Add to dashboard Cite

Visualization of cell migration during chemotaxis using spectral domain optical coherence tomography (OCT) requires non‐standard processing techniques. Stripe artefacts and camera noise floor present in OCT data prevent detailed computer‐assisted reconstruction and quantification of cell locomotion. Furthermore, imaging artefacts lead to unreliable results in automated texture based cell analysis. Here we characterize three pronounced artefacts that become visible when imaging sample structures with high dynamic range, e.g. cultured cells: (i) time‐varying fixed‐pattern noise; (ii) stripe artefacts generated by background estimation using tomogram averaging; (iii) image modulations due to spectral shaping. We evaluate techniques to minimize the above mentioned artefacts using an 800 nm optical coherence microscope. Effect of artefact reduction is shown exemplarily on two cell cultures, i.e. Dictyostelium on nitrocellulose substrate, and retinal ganglion cells (RGC‐5) cultured on a glass coverslip. Retinal imaging also profits from the proposed processing techniques. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

“…Additionally, it may be of interest in cases where interferometric phase retrieval is needed (e.g. in Doppler OCT [27] or spectral domain phase microscopy [28]). We believe the setups presented here are scalable and therefore applicable to endoscopy.…”

Section: Introductionmentioning

confidence: 99%

Common path Fourier domain optical coherence tomography based on multiple reflections within the sample arm

Krstajić

Hogg

Matcher

2011

Optics Communications

View full text Add to dashboard Cite

We present a common path Fourier domain optical coherence tomography (FDOCT) setup where the reference signal arises from multiple reflections within the sample arm. Two configurations are demonstrated. The first is based on a reflective microscope objective while the second is based on a normal (refractive) microscope objective. The second configuration is effectively a Mireau interferometer. We present sensitivity analysis of these setups and images of in vivo skin. Advantages of both common path arrangements include: 1) the reference surface is not close to the sample surface while keeping the optical path lengths matched (so the additional interferometer is not needed) and 2) the user can independently control reference and sample arm power. Additionally, the configuration using the refractive objective ensures that the coherence gate and focus gate always match. A disadvantage is that the reference arm power in certain circumstances is not optimal (i.e. close to saturating the CCD). However, this issue can be removed by a light source of sufficient output power. We believe the idea is scalable and therefore of interest to endoscopy applications. 3 RESEARCH HIGHLIGHTS• Multiple reflections within the sample arm can be beneficial.• We demonstrate a novel common-path Fourier domain OCT based on Mireau interferometer.

show abstract

Spectral-domain phase microscopy

Cited by 331 publications

References 7 publications

Spectral-domain optical coherence phase microscopy for quantitative phase-contrast imaging

Spectral-domain optical coherence phase microscopy for quantitative phase-contrast imaging

Artefact reduction for cell migration visualization using spectral domain optical coherence tomography

Common path Fourier domain optical coherence tomography based on multiple reflections within the sample arm

Contact Info

Product

Resources

About