Spectral phasor analysis reveals altered membrane order and function of root hair cells in Arabidopsis dry2/sqe1-5 drought hypersensitive mutant

Interactions between lipids and proteins are critical for organization of plasma membrane-ordered domains in tobacco BY-2 cells

“…The complex mechanisms by which the PM is synthesized and renewed might be a way to control the targeting of the different membrane components ( Zarský et al ., 2009 ; Kleine-Vehn et al , 2011 ), and consequently to control the interactions between neighbouring lipids and proteins. In accordance with this, modifications of PM composition in an Arabidopsis mutant have been reported to alter membrane order at the resting state ( Sena et al , 2017 ). According to the limited data available, such a metabolic turnover of membrane components might occur over a time frame of several hours ( Shi et al , 2008 ; Stanislas et al , 2009 ; Chalbi et al , 2015 ), which far exceeds our experimental conditions.…”

Ethylene and hydrogen peroxide regulate formation of a sterol-enriched domain essential for wall labyrinth assembly in transfer cells

“…However, in the case of ETC wall labyrinth formation, the very disparate assembly patterns of the UWL and WI papillae (Xia et al , 2017) suggest that SED composition/spatial organization must undergo substantive shifts as wall labyrinth formation progresses from UWL to WI papillae construction. In the case of SED composition, there is a shift in the expression of genes encoding sterol biosynthesis between the phases of wall labyrinth formation (see Supplementary Table S5B) that could impact vesicle trafficking and PM properties (Pook et al , 2017; Sena et al , 2017). Moreover, UWL formation is characterized by an even deposition of matrix polysaccharides across the outer periclinal original wall and is independent of cellulose biosynthesis.…”

In vivo macromolecular crowding is differentially modulated by Aquaporin 0 in zebrafish lens: insights from a nano-environment sensor and spectral imaging

“…Most importantly though, spectral phasors follow rules of vector algebra, known as the linear combination of phasors (30,54). This property refers to the additivity of components and allows the geometrical calculation of mixed pure environments.…”