Spectral sensitivity ofCalliphora erythrocephala and other insect species studied with Fourier Interferometric Stimulation (FIS)

Paul, Rüdiger J.; Steiner, Andreas; Gemperlein, R.

doi:10.1007/bf00603824

Cited by 29 publications

(22 citation statements)

References 47 publications

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“…Most rapid, computer-based methods of measuring spectral sensitivities have been AC or DC constant-response methods (the exception is Fourier interferometry based on linear responses; this method also requires measurements of cell frequency responses (22) Baseline instabilities, which are frequent in intracellular recordings due to movement of the animal, muscle contractions or other unwanted events, affect the DC constantresponse method because they cause fluctuations which are falsely interpreted as sensitivity changes. The AC constant-response method has the advantage of being relatively independent of baseline shifts, since the criterion is a peak-to-peak amplitude rather than a voltage level.…”

Section: Discussionmentioning

confidence: 99%

“…The computerized Fourier interferometric method of Gemperlein and colleagues (18)(19)(20)(21)(22)(23) appears to be the only application of the linear response method. In the interferometric method, schematized in Figure 2E, a continuously scanning Michelson interferometer generates stimulus and response interferograms in a range of wavelengths which are frequency coded.…”

Section: Linear-response Methodsmentioning

confidence: 99%

“…By means of the Fast Fourier Transform (FFT), the amplitudes as a function of frequency are resolved into amplitudes as functions of wavelength. After appropriate corrections, the spectral sensitivities are determined by dividing the response amplitudes by the stimulus amplitudes (22). Complete spectral sensitivities for 40 wavelengths in the range of 300 to 700 nm can be determined in 7 to 16 s. One complication of this method is that response amplitudes within the frequency bands of the interferogram may depend not only upon wavelength but also upon the frequency response of the cell.…”

Section: Linear-response Methodsmentioning

confidence: 99%

“…The dependence of amplitudes on frequencies has proven small for the frequencies used (4)(5)(6)(7)(8) and the photoreceptors tested (21), but the method does require that the frequency responses be measured separately using, for example, sinusoidally modulated light. Nonlinearities in response, if any, show up at second and higher harmonics of the frequencies in the interferograms and can either be ignored or used for further system description (22).…”

Section: Linear-response Methodsmentioning

confidence: 99%

“…Methods which measure S(λ) during continuous illumination (14,22) eliminate sensitivity variations introduced by changes in the adaptation state as is also the case for constant-response methods. In constant-response methods, illumination is also continuous and the light intensity at each wavelength is adjusted until the response (AC or DC) matches a criterion amplitude.…”

Section: Constant-response Methodsmentioning

confidence: 99%

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Electrophysiological measurements of spectral sensitivities: a review

DeVoe

de-Souza²,

Ventura³

1997

Braz J Med Biol Res

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Spectral sensitivities of visual systems are specified as the reciprocals of the intensities of light (quantum fluxes) needed at each wavelength to elicit the same criterion amplitude of responses. This review primarily considers the methods that have been developed for electrophysiological determinations of criterion amplitudes of slow-wave responses from single retinal cells. Traditional flash methods can require tedious dark adaptations and may yield erroneous spectral sensitivity curves which are not seen in such modifications as ramp methods. Linear response methods involve interferometry, while constant response methods involve manual or automatic adjustments of continuous illumination to keep response amplitudes constant during spectral scans. In DC or AC computerized constant response methods, feedback to determine intensities at each wavelength is derived from the response amplitudes themselves. Although all but traditional flash methods have greater or lesser abilities to provide on-line determinations of spectral sensitivities, computerized constant response methods are the most satisfactory due to flexibility, speed and maintenance of a constant adaptation level. Correspondence

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Section: Discussionmentioning

confidence: 99%

Section: Linear-response Methodsmentioning

confidence: 99%

Section: Linear-response Methodsmentioning

confidence: 99%

Section: Linear-response Methodsmentioning

confidence: 99%

Section: Constant-response Methodsmentioning

confidence: 99%

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Electrophysiological measurements of spectral sensitivities: a review

DeVoe

de-Souza²,

Ventura³

1997

Braz J Med Biol Res

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A butterfly eye's view of birds

Frentiu

Briscoe

2008

BioEssays

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The striking color patterns of butterflies and birds have long interested biologists. But how these animals see color is less well understood. Opsins are the protein components of the visual pigments of the eye. Color vision has evolved in butterflies through opsin gene duplications, through positive selection at individual opsin loci, and by the use of filtering pigments. By contrast, birds have retained the same opsin complement present in early-jawed vertebrates, and their visual system has diversified primarily through tuning of the short-wavelength-sensitive photoreceptors, rather than by opsin duplication or the use of filtering elements. Butterflies and birds have evolved photoreceptors that might use some of the same amino acid sites for generating similar spectral phenotypes across approximately 540 million years of evolution, when rhabdomeric and ciliary-type opsins radiated during the early Cambrian period. Considering the similarities between the two taxa, it is surprising that the eyes of birds are not more diverse. Additional taxonomic sampling of birds may help clarify this mystery.

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Is the white eye of insect eye-color mutants really white?

Gribakin

Ukhanov

1990

J Comp Physiol A

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1. Spectral sensitivity curves (SSCs) of (a) the whole eye and peripheral photoreceptors R1-6 were recorded in wild-type flies and chalky mutants of Calliphora, and (b) the whole eye of Drosophila, wild type and mutant white. Our spectrosensitometer provided good resolution and accuracy.2. A vibrational fine structure of the SSC in the ultraviolet spectral range was clearly resolved in all the genotypes studied (Fig. 1).3. In the visible spectral range, the SSCs of whiteeyed mutants appeared to be significantly narrower than the absorption spectrum of the template visual pigments (Fig. 2). The bandwidth ratios (wild type: visual pigment: white-eyed mutant) were as follows: 111_ 10:102:95_4nm and 114+__14"102:93+7 nm in Calliphora whole eye and cells R1-6, respectively; and 118 • 6:100:97___ 2 nm in the whole eye of Drosophila (Fig. 3).4. Long-wave portions of SSCs in mutants showed a good fit with those of the absorption spectrum of visual pigments: P490 for the whole eye, P487 for receptors RI-6 of chalky mutants of Calliphora, and P478 for the whole eye of Drosophila.5. The narrowing of the SSCs compared with the absorption spectrum of the template visual pigment is believed to be due to the presence of hypothetical blueabsorbing pigments in the eye media.

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Spectral sensitivity ofCalliphora erythrocephala and other insect species studied with Fourier Interferometric Stimulation (FIS)

Cited by 29 publications

References 47 publications

Electrophysiological measurements of spectral sensitivities: a review

Electrophysiological measurements of spectral sensitivities: a review

A butterfly eye's view of birds

Is the white eye of insect eye-color mutants really white?

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