Spectral surface plasmon resonance biosensor for detection of staphylococcal enterotoxin B in milk

Homola, Jiřı́; Dostálek, Jakub; Chen, Shengfu; Rasooly, Avraham; Jiang, Shaoyi; Yee, Sinclair S.

doi:10.1016/s0168-1605(02)00010-7

Cited by 303 publications

(152 citation statements)

References 24 publications

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“…In this study, a custom-built surface plasmon resonance (SPR) sensor was used to measure protein adsorption on the coated substrata [22,23]. A SPR chip was attached to the base of the prism and optical contact was established using refractive index matching fluid (Cargille).…”

Section: Measurements Of Protein Adsorptionmentioning

confidence: 99%

Inhibition of bacterial adhesion and biofilm formation on zwitterionic surfaces

et al. 2007

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In this work, we report a study of long-chain zwitterionic poly(sulfobetaine methacrylate) (pSBMA) surfaces grafted via atom transfer radical polymerization (ATRP) for their resistance to bacterial adhesion and biofilm formation. Previously, we demonstrated that p(SBMA) is highly resistant to nonspecific protein adsorption. Poly(oligo(ethylene glycol) methyl ether methacrylate) (pOEGMA) grafted surfaces were also studied for comparison. Furthermore, we quantify how surface grafting methods will affect the long-term biological performance of the surface coatings. Thus, self-assembled monolayers (SAMs) of alkanethiols with shorter-chain oligo(ethylene glycol) (OEG) and mixed SO 3 − /N + (CH 3 ) 3 terminated groups were prepared on gold surfaces. The short-term adhesion (3 h) and the long-term accumulation (24 h or 48 h) of two bacterial species (Gram-positive Staphylococcus epidermidis and Gram-negative Pseudomonas aeruginosa) on these surfaces were studied using a laminar flow chamber. Methyl (CH 3 ) SAM on gold and a bare glass were chosen as references. p(SBMA) reduced short-term adhesion of S. epidermidis and P. aeruginosa relative to glass by 92% and 96%, respectively. For long-term biofilm formation, qualitative images showed that p(SBMA) dramatically reduced biofilm formation of S. epidermidis and P. aeruginosa as compared to glass.

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Section: Measurements Of Protein Adsorptionmentioning

confidence: 99%

Inhibition of bacterial adhesion and biofilm formation on zwitterionic surfaces

et al. 2007

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“…SPR biosensors have found extensive application in the analysis of biomolecular interactions (BIA) and detection of chemical and biological analytes [1], where they provide benefits of real-time, sensitive and label-free technology. They have been used for detection of various chemical and biological compounds in areas such as environmental protection [2], food safety [3], [4] and medical diagnostics [5].…”

Section: Introductionmentioning

confidence: 99%

Design and optimization of a novel surface plasmon resonance biosensor based on Otto configuration

Akowuah¹,

Gorman²,

Haxha³

2009

Opt. Express

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Abstract:A theoretical study on a novel planar waveguide surface Plasmon Biosensor is presented in this paper. The proposed biosensor has a configuration similar to the Otto excitation mechanism for surface Plasmon polaritons. The performance of the device with respect to key system parameters such as gap -width and device length is investigated using an eigenmode solver with perfectly matched layers (PML). Device resolution of 2.3×10-6 RIU has been demonstrated for an aqueous analyte.

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“…This device was used to directly detect staphylococcal enterotoxin B in milk. Sensitivity was increased when a secondary antibody was used to amplify the signal (129).…”

Section: Immunological Detectionmentioning

confidence: 99%

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

et al. 2005

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SUMMARY Recent events have made public health officials acutely aware of the importance of rapidly and accurately detecting acts of bioterrorism. Because bioterrorism is difficult to predict or prevent, reliable platforms to rapidly detect and identify biothreat agents are important to minimize the spread of these agents and to protect the public health. These platforms must not only be sensitive and specific, but must also be able to accurately detect a variety of pathogens, including modified or previously uncharacterized agents, directly from complex sample matrices. Various commercial tests utilizing biochemical, immunological, nucleic acid, and bioluminescence procedures are currently available to identify biological threat agents. Newer tests have also been developed to identify such agents using aptamers, biochips, evanescent wave biosensors, cantilevers, living cells, and other innovative technologies. This review describes these current and developing technologies and considers challenges to rapid, accurate detection of biothreat agents. Although there is no ideal platform, many of these technologies have proved invaluable for the detection and identification of biothreat agents.

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Spectral surface plasmon resonance biosensor for detection of staphylococcal enterotoxin B in milk

Cited by 303 publications

References 24 publications

Inhibition of bacterial adhesion and biofilm formation on zwitterionic surfaces

Inhibition of bacterial adhesion and biofilm formation on zwitterionic surfaces

Design and optimization of a novel surface plasmon resonance biosensor based on Otto configuration

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

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