Spectrophotometric and Stability Indicating High Performance Liquid Chromatographic Determination of Nortriptyline Hydrochloride and Fluphenazine Hydrochloride

El‐Ragehy, Nariman A.; Abbas, Samah S.; El-khateeb, Sonia Z.

doi:10.1081/al-120005971

Cited by 14 publications

(11 citation statements)

References 27 publications

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“…Recovery determination for bulk powders applying standard addition technique (Table 3) gave good results. The obtained results were statistically compared with those obtained by applying a reference method, [34] in which HPLC-method was used for determination of the analytes mixture using a conventional RP-C18. In the reference method, [34] the mobile phase was prepared by mixing 0.05 M ammonium acetate in distilled water: methanol: acetonitrile in a ratio (4: 1: 5) at flow rate of 1 mL min À1 ; the pH was adjusted to 7.5 AE 0.1.…”

Section: Simultaneous Hplc-determination Of Nortriptyline and Fluphenmentioning

confidence: 99%

Simultaneous HPLC-Determination of Nortriptyline and Fluphenazine in One Minute Using Monolithic Stationary Phase

Hashem

Jira

2013

Journal of Liquid Chromatography & Related Technologies

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& A simple, fast, robust, and accurate high-performance liquid chromatography (HPLC) method is described for simultaneous quantification of nortriptyline and fluphenazine in bulk powder and dosage forms. The chromatographic separation was carried out in less than one minute on Chromolith 1 Performance RP-18e column, which consists of monolithic rods of highly porous silica, using isocratic binary mobile phase of MeOH and 25 mM KH 2 PO 4 pH 4.5 in the ratio of 70: 30 at 5 mL min À1 flow rate and 40 C. The high porosity of stationary phase enables it to be used at high flow rates without problems concerning backpressure, these high flow rates in turn lead to strong reduction of analysis time. A diode array detector was used at 254 nm for detection. The method was validated for system suitability, linearity, precision, limits of detection and quantitation, specificity, and robustness. LOD were found to be 0.40 and 0.78 lg mL À1 for nortriptyline and fluphenazine, respectively. LOQ was 3.125 lg mL À1 for both analytes. Recovery values of this method were 98.50 and 98.00% for nortriptyline and fluphenazine, respectively, and reproducibility was within 2.36%. Robustness study was done for small changes in KH 2 PO 4 concentration and pH, temperature, flow rate, wavelength of detection, % of MeOH in mobile phase, and injection volume.

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Section: Simultaneous Hplc-determination Of Nortriptyline and Fluphenmentioning

confidence: 99%

Simultaneous HPLC-Determination of Nortriptyline and Fluphenazine in One Minute Using Monolithic Stationary Phase

Hashem

Jira

2013

Journal of Liquid Chromatography & Related Technologies

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“…These techniques include fluorimetry [7], colorimetry [8], electrogenerated chemiluminescence [9], thin-layer chromatography [10], gas chromatography [11], high-performance liquid chromatography [10,[12][13][14][15][16][17], fast Fourier transform continuous cyclic voltammetry [18], stripping voltammetry [19] and capillary zone electrophoresis [20,21]. Certain hyphenated techniques such as ultra performance liquid chromatography-tandem mass spectrometry [22], gas chromatography-mass spectrometry [23,24] and dispersive liquid-liquid microextraction combined with gas chromatography [11] have been reported for the assay of nortriptyline.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Modelling for the Assay of Nortriptyline Hydrochloride Using Potassium Permanganate as Oxidant

Rahman

Khan

2014

AAPS PharmSciTech

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Kinetic methods for accurate determination of nortriptyline hydrochloride have been described. The methods are based on the oxidation of nortriptyline hydrochloride with KMnO4 in acidic and basic media. In acidic medium, the decrease in absorbance at 525.5 nm and in basic medium, the increase in absorbance at 608.5 nm were measured as a function of time. The variables affecting the reactions were carefully investigated and optimised. Kinetic models such as initial rate, rate constant, variable time and fixed time were employed to construct the calibration curves. The initial rate and fixed time methods were selected for quantification of nortriptyline hydrochloride. In acidic medium, the calibration curves showed a linear response over the concentration range 10-50 μg mL(-1) for initial rate and 10-60 μg mL(-1) for fixed time method (2 min). In basic medium, the calibration graphs were linear over the concentration range 10-100 μg mL(-1) for initial rate and fixed time methods (4 min). In acidic medium, the limits of detection for initial rate and fixed time methods (2 min) were 1.02 and 3.26 μg mL(-1), respectively. In basic medium, the limits of detection were found to be 1.67 and 1.55 μg mL(-1) for initial rate and fixed time methods (4 min), respectively. The initial rate and fixed time methods have been successfully applied to the determination of nortriptyline hydrochloride in commercial dosage form. Statistical comparison of the results of the proposed methods with those of reference method exhibited excellent agreement and there is no significant difference between the compared methods in terms of accuracy and precision.

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“…Numerous analytical techniques have been noticed in the literature for the analysis of FPZ. The techniques include spectrophotometry [ 5 ], spectrofluorimetry [ 6 ], turbidmetric [ 7 ], gas chromatography (GC) [ 8 ], HPTLC [ 9 , 10 ], etc. A number of high-performance liquid chromatography (HPLC) methods have also been reported for this drug using ultraviolet (UV) as well as mass (MS) detectors [ 11 – 16 ].…”

Section: Introductionmentioning

confidence: 99%

Forced Degradation Behaviour of Fluphenazine Hydrochloride by LC and Characterization of its Oxidative Degradation Product by LC–MS/MS

et al. 2015

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A novel, stability-indicating high-performance liquid chromatographic (HPLC) method is delivered for the determination of fluphenazine hydrochloride (FPZ) and its degradation products. The forced degradation testing of FPZ was carried out for hydrolytic, oxidative, photolytic, and thermal degradation. The degradation appeared using a reversed-phase C18 column at ambient temperature with a mobile phase comprised of methanol : acetonitrile : (10 mM) ammonium acetate (70:15:15, v/v/v) pH 6.0, adjusted with acetic acid, having a flow rate of 1 ml min−1 and a detection wavelength at 259 nm. Primarily, the maximum degradation products were formed under oxidative stress conditions. The product was distinguished through LC-MS/MS fragmentation studies. Based on the results, a more complete degradation pathway for the drug could be proposed. The modernized method was found to be precise, accurate, specific, and selective. The method was found to be suitable for the quality control of fluphenazine hydrochloride in the tablet as well as in stability-indicating studies.

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Spectrophotometric and Stability Indicating High Performance Liquid Chromatographic Determination of Nortriptyline Hydrochloride and Fluphenazine Hydrochloride

Cited by 14 publications

References 27 publications

Simultaneous HPLC-Determination of Nortriptyline and Fluphenazine in One Minute Using Monolithic Stationary Phase

Simultaneous HPLC-Determination of Nortriptyline and Fluphenazine in One Minute Using Monolithic Stationary Phase

Kinetic Modelling for the Assay of Nortriptyline Hydrochloride Using Potassium Permanganate as Oxidant

Forced Degradation Behaviour of Fluphenazine Hydrochloride by LC and Characterization of its Oxidative Degradation Product by LC–MS/MS

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