Spectrophotometric Determination of Dapsone Using Charge Transfer Complex Formation Reaction

Abstract

A sImple, accurate and sensitive spectrophotometric method for the determination of Dapsone has been developed. The method is based on the charge transfer complex formation reaction of dapsone as n-donors with o-chloranil (o-CA) as π-acceptor in aqueous medium forming colored complex with maximum absorption at 394 nm. Beer's law was obeyed in concentration ranges of 0.125-1.25 µg/ml with molar absorptivity 1.092×10 5 l. mol -1 .cm -1 . The accuracy of the method (average recovery %) was 100.34% and precision (RsD) ≤ 3.91 %. The results show that complex is formed in ratio 1:2 with the stability constant 3.81×10 9 l 2 .mol -2 . The suggested method was applied successfully to determine Dapsone in its pharmaceutical preparation.

Determination of trace amount of some drugs as in its pure form and in its Pharmaceutical Preparations by using different spectroscopic methods

“…These reactions are based on the pharmacokinetics' activity as an oxidative or reduced agent in a specific reaction, followed by the creation of a colored substance that can be used in quantitative determination. Paracetamol (32), trimethoprim (29), acebutolol, butyranilinde (30), and ascorbic acid (31) are the drugs tested using this process. For example the following equation:…”

Determination of Salbutamol Using Spectrophotometric Method Depending on Chromium-1,5-diphenylcarbazide Complex

“…One of these methods has used 4-amino antipyrine in basic with maximum absorption at 500nm [2], Another method has studied the determination of SAL in tablets [3] or using N-Bromo succinimide to assay SAL in bulk and tablet dosage forms [4]. Moreover, fluorescence method was β-cyclodextrin with SAL at emission wavelength 609.8 nm [5], charge-transfer reaction with o-chloranil has been used for estimating of SAL [6], or on the coupling between N, N-diethyl-p-phenylenediamine Sulfate with SAL in the presence of KIO 4 [7], Also, ion-pair formation between SAL and Hematoxylin at pH 9.5 [8]. The spectrophotometric method has been developed for the determination of SAL in tablet [9], 2-chloro-4-nitroaniline was reacted with Nitrite in acidic medium to form produce diazonium ion, which is finally coupled with SAL in basic medium forming azo dyes [10], Other researches were described a new method to determine SAL in bulk drug and tablet dosage [11], coupling reaction was used with diazotized O-nitroaniline in alkaline medium to estimate SAL [12], flow injection methods with spectrophotometric detection have been used for determining of SAL in pharmaceutical formulations [13].…”