Spectrophotometric Method for Simultaneous Determination of Amiloride and Hydrochlorothiazide in Combined Dosage Form

Abstract

A Simple, precise, accurate and economical spectrophotometric method was developed and validated for simultaneous estimation of Amiloride (AML) and Hydrochlorothiazide (HCZ) in combined dosage form. In simultaneous equation method, AML and HCZ were quantified using their absorptivity values at selected wavelengths, viz., 244nm and 280nm respectively in 0.1 N NaOH. The linearity range was found to be 5-25 μg/ml for AML and HCZ. The accuracy and reproducibility of the proposed method was statistically validated by recovery studies. The simultaneous equation method permits simple, rapid and direct determination of AML and HCZ in commercially available combined dosage form without previous separations and can therefore be used for routine analysis.

Spectrophotometric Assay Methods for Determination of Amiloride: A Charge-Transfer Reaction Approach with 2,3-Dichloro-5,6-Dicyano-1,4-Benzoquinone and p-Chloranilic Acid Reagents

“…Because the form of derivative spectra and zero order spectra is similar, a tiny alteration in a basic spectrum can dramatically modify the derivative spectrum. Poor reproducibility can affect findings when multiple spectrophotometers used for zero order spectra produce comparable results but derivatization produces different results [1][2][3][4][5][6][7][8][9][10] . In view of developing simple, straightforward and sensitive spectrophotometric techniques have been developed and validated by the authors of the current work for the analysis of Amiloride hydrochloride (AMD) in pure and pharmaceutical dosage forms; p-Chloranilic Acid (PCA) and 2,3-dichloro-5,6dicyano-1,4-benzoquinone (DDQ) are used as π-acceptors.…”