Spectrophotometric study of complexation between betulin 3,28-diphthalate and β-cyclodextrin

Sursyakova, Viktoria V.; Maksimov, N. G.; Levdansky, Vladimir A.; Rubaylo, Anatoly I.

doi:10.1007/s10847-023-01182-5

Cited by 3 publications

(1 citation statement)

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“…The binding constants of complexes can be determined by several analytical and physico-chemical methods, for example, equilibrium dialysis [7,8], isothermal titration calorimetry [7,9,10], spectrophotometry [11], fluorescence spectrometry [9], surface plasmon resonance [12], nuclear magnetic resonance [13,14], mass spectrometry [8,14,15], affinity chromatography [6,16], slab gel electrophoresis [17,18], and thermophoresis [19]. In addition, several CE methods can be used for the determination of the binding constants of complexes, especially the different modes of affinity CE (ACE) [19][20][21], such as mobility shift ACE (ms-ACE) [22][23][24][25], Hummel-Dreyer method [26], ms vacancy ACE [22], frontal analysis CE [7,9,25,27], continuous frontal analysis CE [26,28], partial filling ACE [29][30][31][32] and kinetic CE [33,34].…”

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confidence: 99%

Determination of the binding constants and ionic mobilities of diquat complexes with randomly sulfated cyclodextrins by affinity capillary electrophoresis

Bílek,

Koval,

Šolínová

et al. 2024

J of Separation Science

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The enantiomers of diquats (DQs), a new class of functional organic molecules, were recently separated by capillary electrophoresis (CE) with high resolution up to 11.4 within 5–7 min using randomly sulfated α‐, β‐, and γ‐cyclodextrins (CDs) as chiral selectors. These results indicated strong interactions between dicationic diquats and multiply negatively charged sulfated CDs (S‐CDs). However, the binding strength of these interactions was not quantified. For that reason, in this study, affinity CE was applied for the determination of the binding constants and ionic mobilities of the complexes of DQ P‐ and M‐enantiomers with CD chiral selectors in an aqueous medium. The non‐covalent interactions of 10 pairs of DQ enantiomers with the above CDs were investigated in a background electrolyte (BGE) composed of 22 mM NaOH, 35 mM H3PO4, pH 2.5, and 0.0–1.0 mM concentrations of CDs. The average apparent binding constant and the average actual ionic mobility of the DQ‐CD complexes were determined by nonlinear regression analysis of the dependence of the effective mobility of DQ enantiomers on the concentration of CDs in the BGE. The complexes were found to be relatively strong with the averaged apparent binding constants in the range 13 600–547 400 L/mol.

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