Spectroscopic analysis of the binding of doxorubicin to human .alpha.-1 acid glycoprotein

The Hedgehog Receptor Patched Functions in Multidrug Transport and Chemotherapy Resistance

“…We confirmed these results by looking at the doxorubicin fluorescence quenching in solution, which is another way to measure doxorubicin incorporation in cells. Indeed, the intercalation of the chromophoric rings of doxorubicin between adjacent DNA base pairs has been shown to cause significant reduction in the fluorescence of the drug (26). We observed that the addition of K562 cells pretreated with CPN to a doxorubicin solution induced more fluorescence quenching than the addition of untreated cells.…”

“…These results are in full agreement with those obtained for growth inhibition, and indicate that hPtc facilitates the efflux of doxorubicin, acriflavine, and H33342. We then prepared membrane extracts to measure the binding of doxorubicin and acriflavine, looking at the fluorescence quenching caused by the incorporation of the dyes in a hydrophobic environment (26). Membrane prepared from yeasts expressing hPtc caused a faster fluorescence quenching than membrane prepared from control yeasts, suggesting that doxorubicin and acriflavine bound to hPtc (Fig.…”

Nonaqueous affinity capillary electrophoresis investigation of small molecule molecular recognition

“…The synthesis of the p-xylylenyl bis-iminoimidazolinium receptor was modeled after a procedure from the literature [39]. 1 H-NMR as well as electron impact (EI) and fast atom bombardment (FAB) mass spectra were taken to establish the purity and identity of the p-XDI receptor.…”

“…The functioning of a variety of biological, chemical, and biochemical systems hinges upon these interactions, thus a greater understanding of these complex systems may be acquired better through the examination of interactions at the molecular level. A variety of techniques have been applied to probe molecular interactions, both qualitatively and quantitatively, including spectroscopy [1][2][3][4], potentiometry [5,6], separations [7][8][9], and nuclear magnetic resonance (NMR) [10][11][12][13][14][15]. A relatively new approach for identifying and quantifying the extent of molecular binding events is a technique known as affinity capillary electrophoresis (ACE) [16][17][18][19][20][21][22].…”

Mechanistic aspects of chiral discrimination by surface-immobilized ?1-acid glycoprotein

“…17,19 Drug binding has been studied using various techniques which include circular dichroism, UV absorbance and fluorescence spectroscopy, HPLC, and calorimetry. [20][21][22][23] The predominant nature of the interaction of drugs with AGP appears to be hydrophobic. [13][14][15] Some investigators, however, have proposed that electrostatic, 23 van der Waal's, 22 and hydrogen bonding 24 interactions participate in drug binding as well.…”