Spectroscopic, anticancer and antioxidant studies of fluxional trans-[PdCl2(S-acylthiourea)2] complexes

Dorairaj, Dorothy Priyanka; Haribabu, Jebiti; Vadivalagan, Chithravel; Vennila, K.N.; Bhuvanesh, Nattamai; Echeverría, César; Hsu, Sodio C. N.; Karvembu, Ramasamy

doi:10.1016/j.rechem.2021.100157

Cited by 19 publications

(11 citation statements)

References 88 publications

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“…Electronic absorption spectroscopy is an effective method for examining how substances bind to DNA 26 . Covalent and noncovalent interactions between DNA and transition metal complexes are the two main types of interactions.…”

Section: Resultsmentioning

confidence: 99%

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Synthesis, characterization, cytotoxicity evaluation, and molecular docking with DNA, bovine serum albumin, and SARS‐Cov‐2 of copper complexes bearing tris‐(2‐pyridyl)‐pyrazolyl borate ligand

Narwane

Dorairaj

Roymahapatra

et al. 2023

Applied Organom Chemis

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Four copper (II) complexes bearing tris‐(2‐pyridyl)‐pyrazolyl borate (Tppy) ligand with corresponding chloride (Cu‐1), aqua (Cu‐2), azide (Cu‐3), and thiocyanide (Cu‐4) substitutions were synthesized and characterized by spectroscopic and analytical methods. Spectroscopic and molecular docking studies were employed to investigate the interactions of these complexes with calf thymus (CT) DNA and bovine serum albumin (BSA). The results inferred intercalation binding mode of the complexes with DNA. All the complexes exhibited good binding with BSA as well. In addition, the binding efficacy of the Cu (II) complexes with SARS‐Cov‐2 was tested in silico. Further, in vitro anticancer activity of the complexes was investigated against the HeLa‐cervical, HepG2‐liver and A549‐lung cancer, and one normal (L929‐fibroblast) cell line. IC50 values unveiled that the complexes were more active than cisplatin against all three cancer cells. It was understood that complex Cu‐3 containing azide substitution displayed the highest activity on the HeLa cell line (IC50 = 6.3 μM). More importantly, TppyCu (II) complexes were not active against the normal cell line. Lastly, the acridine orange/ethidium bromide (AO/EB) and 4′,6‐diamidino‐2‐phenylindole staining assays indicated that Cu‐3 induced cell death in HeLa cells at the late apoptotic stage. This complex also efficiently generated ROS in HeLa cells promoting apoptosis as understood from the DCFH‐DA assay.

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Section: Resultsmentioning

confidence: 99%

“…Electronic absorption spectroscopy is an effective method for examining how substances bind to DNA. 26 Covalent and noncovalent interactions between DNA and transition metal complexes are the two main types of interactions. In general, covalent DNA binding is irreversible and always results in total DNA blockage and apoptosis.…”

Section: Binding With Ct Dnamentioning

confidence: 99%

Synthesis, characterization, cytotoxicity evaluation, and molecular docking with DNA, bovine serum albumin, and SARS‐Cov‐2 of copper complexes bearing tris‐(2‐pyridyl)‐pyrazolyl borate ligand

Narwane

Dorairaj

Roymahapatra

et al. 2023

Applied Organom Chemis

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“…The quenching was an indication that the complexes interacted with BSA, and this phenomenon took place because the tryptophan residues of BSA were buried in a hydrophobic environment. [ 45 ] The extent of quenching was calculated from the Stern‐Volmer equation F ° /F = 1 + K q [Q], where F ° and F demonstrate the fluorescence intensities in the absence and presence of quencher respectively, K q is a linear Stern‐Volmer quenching constant, and [Q] is the quencher concentration.…”

Section: Resultsmentioning

confidence: 99%

Section: Binding With Bovine Serum Albumin (Bsa)mentioning

confidence: 99%

Pd(II)–PPh₃ complexes of halogen substituted acylthiourea ligands: Biomolecular interactions and in vitro anti‐proliferative activity

Dorairaj

Haribabu

Chang

et al. 2022

Applied Organom Chemis

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Herein, we report the synthesis of four new Pd(II) acylthiourea complexes (C1–C4) to study the effect of halogen substituted acylthiourea ligands on the biological applications of their Pd(II)–PPh3 complexes. The complexes were characterized by various spectroscopic and analytical methods. The distorted square planar geometry of complexes was confirmed by single crystal X‐ray diffraction study, which also revealed the bidentate (N, S) coordination of the ligands with Pd(II). Interactions of the complexes with biomolecules (DNA/BSA) were investigated by spectroscopic and docking tools. All the complexes showed good binding ability with the targeted biomolecules. Further, in vitro anti‐proliferative activity of the complexes was investigated by performing MTT assay on cancer (HeLa‐cervical, HCT116‐colorectal, and HepG2‐hepatic) and normal (HEK293‐embryonic kidney) cell lines. All the complexes displayed remarkable activity on the three cancer cells, and among them, complex C2 bearing chlorine substituted acylthiourea showed superior activity on HeLa cells with an IC50 value of 6.5 μM, which was higher than that of cisplatin. Apoptosis induced by C2 on HeLa cells was assessed by AO/EB, DAPI and DNA laddering assays, and flow cytometry. Finally, the apoptotic efficiency of C2 was tested by cell cycle analysis, wherein the complex induced the cell cycle arrest in HeLa cells at G0/G1 phase.

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“…A precise way of viewing the structural changes taking place in CT DNA is by means of the hydrodynamic study and likewise here the three zinc(II) complexes were subjected to this study. A typical intercalator or an aromatic compound in general tends to slide in between the base pairs of DNA causing breaking of hydrogen bonds in the DNA base pairs thereby lengthening the DNA helix resulting in increased viscosity values [ 53 ]. A compound binding to CT DNA via the groove mode causes negligent changes in viscosity values.…”

Section: Resultsmentioning

confidence: 99%

Tris-(2-pyridyl)-pyrazolyl Borate Zinc(II) Complexes: Synthesis, DNA/Protein Binding and In Vitro Cytotoxicity Studies

et al. 2021

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Zn(II) complexes bearing tris[3-(2-pyridyl)-pyrazolyl] borate (Tppy) ligand (1–3) was synthesized and examined by spectroscopic and analytical tools. Mononuclear [TppyZnCl] (1) has a Zn(II) centre with one arm (pyrazolyl-pyridyl) dangling outside the coordination sphere which is a novel finding in TppyZn(II) chemistry. In complex [TppyZn(H2O)][BF4] (2) hydrogen bonding interaction of aqua moiety stabilizes the dangling arm. In addition, solution state behaviour of complex 1 confirms the tridentate binding mode and reactivity studies show the exogenous axial substituents used to form the [TppyZnN3] (3). The complexes (1–3) were tested for their ability to bind with Calf thymus (CT) DNA and Bovine serum albumin (BSA) wherein they revealed to exhibit good binding constant values with both the biomolecules in the order of 104–105 M−1. The intercalative binding mode with CT DNA was confirmed from the UV-Visible absorption, viscosity, and ethidium bromide (EB) DNA displacement studies. Further, the complexes were tested for in vitro cytotoxic ability on four triple-negative breast cancer (TNBC) cell lines (MDA-MB-231, MDA-MB-468, HCC1937, and Hs 578T). All three complexes (1–3) exhibited good IC50 values (6.81 to 16.87 μM for 24 h as seen from the MTS assay) results which indicated that these complexes were found to be potential anticancer agents against the TNBC cells.

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Spectroscopic, anticancer and antioxidant studies of fluxional trans-[PdCl2(S-acylthiourea)2] complexes

Cited by 19 publications

References 88 publications

Synthesis, characterization, cytotoxicity evaluation, and molecular docking with DNA, bovine serum albumin, and SARS‐Cov‐2 of copper complexes bearing tris‐(2‐pyridyl)‐pyrazolyl borate ligand

Synthesis, characterization, cytotoxicity evaluation, and molecular docking with DNA, bovine serum albumin, and SARS‐Cov‐2 of copper complexes bearing tris‐(2‐pyridyl)‐pyrazolyl borate ligand

Pd(II)–PPh₃ complexes of halogen substituted acylthiourea ligands: Biomolecular interactions and in vitro anti‐proliferative activity

Tris-(2-pyridyl)-pyrazolyl Borate Zinc(II) Complexes: Synthesis, DNA/Protein Binding and In Vitro Cytotoxicity Studies

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Spectroscopic, anticancer and antioxidant studies of fluxional trans-[PdCl2(S-acylthiourea)2] complexes

Cited by 19 publications

References 88 publications

Synthesis, characterization, cytotoxicity evaluation, and molecular docking with DNA, bovine serum albumin, and SARS‐Cov‐2 of copper complexes bearing tris‐(2‐pyridyl)‐pyrazolyl borate ligand

Synthesis, characterization, cytotoxicity evaluation, and molecular docking with DNA, bovine serum albumin, and SARS‐Cov‐2 of copper complexes bearing tris‐(2‐pyridyl)‐pyrazolyl borate ligand

Pd(II)–PPh3 complexes of halogen substituted acylthiourea ligands: Biomolecular interactions and in vitro anti‐proliferative activity

Tris-(2-pyridyl)-pyrazolyl Borate Zinc(II) Complexes: Synthesis, DNA/Protein Binding and In Vitro Cytotoxicity Studies

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Pd(II)–PPh₃ complexes of halogen substituted acylthiourea ligands: Biomolecular interactions and in vitro anti‐proliferative activity