Spectroscopic imaging system for high-throughput viability assessment of ovarian spheroids or microdissected tumor tissues (MDTs) in a microfluidic chip

St-Georges-Robillard, Amélie; Masse, Mathieu; Kendall‐Dupont, Jennifer; Strupler, Mathias; Patra, Bishnubrata; Jermyn, Michael; Mes‐Masson, Anne‐Marie; Leblond, Frédéric; Gervais, Thomas

doi:10.1117/12.2211159

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Cellular viability and 3D morphology of the spheroids can be evaluated on-chip by using optical microscopy ( Figure 16A(b) & A(c)) [120,205], fluorescent microscopy ( Figure 16A(b) & A(c)) [8,105,120], the non-destructive spectroscopic imaging [206], confocal microscopy [52,105,173] and scanning electrochemical microscopy (SECM) ( Figure 7A) [151]. In on-chip live/dead fluorescent staining of the spheroids, the projected areas of green and red regions are quantified as live or dead portions of the spheroid following chemosensitivity tests.…”

Section: On-chip Spheroid Analysis Methodsmentioning

confidence: 99%

Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture

Moshksayan

Kashaninejad

Warkiani

et al. 2018

Sensors and Actuators B: Chemical

215

175

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Highlights  Cell spheroids are spherical aggregates best mimicking the tissue microenvironment.  Spheroid culture better recapitulates the in-vivo condition in microfluidic chips.  Microfluidics provides rapid spheroid formation with size uniformity and control.  These chips contain microwells, microstructures, droplet generators, etc.  To fabricate such chips, some design considerations must be taken into account.

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Section: On-chip Spheroid Analysis Methodsmentioning

confidence: 99%

Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture

Moshksayan

Kashaninejad

Warkiani

et al. 2018

Sensors and Actuators B: Chemical

215

175

View full text Add to dashboard Cite

show abstract

“…Finally, making the device with a transparent material (e.g. glass, PDMS) allows observation and analysis of samples in the device by different types of microscopy and wide field imaging 26 . All of these points show how MSTs are particularly effective at sustaining, treating and analysing large numbers of samples simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Simulation-assisted design of microfluidic sample traps for optimal trapping and culture of non-adherent single cells, tissues, and spheroids

Rousset

Monet

Gervais

2017

Sci Rep

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This work focuses on modelling design and operation of “microfluidic sample traps” (MSTs). MSTs regroup a widely used class of microdevices that incorporate wells, recesses or chambers adjacent to a channel to individually trap, culture and/or release submicroliter 3D tissue samples ranging from simple cell aggregates and spheroids, to ex vivo tissue samples and other submillimetre-scale tissue models. Numerous MST designs employing various trapping mechanisms have been proposed in the literature, spurring the development of 3D tissue models for drug discovery and personalized medicine. Yet, there lacks a general framework to optimize trapping stability, trapping time, shear stress, and sample metabolism. Herein, the effects of hydrodynamics and diffusion-reaction on tissue viability and device operation are investigated using analytical and finite element methods with systematic parametric sweeps over independent design variables chosen to correspond to the four design degrees of freedom. Combining different results, we show that, for a spherical tissue of diameter d < 500 μm, the simplest, closest to optimal trap shape is a cube of dimensions w equal to twice the tissue diameter: w = 2d. Furthermore, to sustain tissues without perfusion, available medium volume per trap needs to be 100× the tissue volume to ensure optimal metabolism for at least 24 hours.

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Inventions and Innovations in Preclinical Platforms for Cancer Research

2018

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Three-dimensional (3D) cell culture systems can be regarded as suitable platforms to bridge the huge gap between animal studies and two-dimensional (2D) monolayer cell culture to study chronic diseases such as cancer. In particular, the preclinical platforms for multicellular spheroid formation and culture can be regarded as ideal in vitro tumour models. The complex tumour microenvironment such as hypoxic region and necrotic core can be recapitulated in 3D spheroid configuration. Cells aggregated in spheroid structures can better illustrate the performance of anti-cancer drugs as well. Various methods have been proposed so far to create such 3D spheroid aggregations. Both conventional techniques and microfluidic methods can be used for generation of multicellular spheroids. In this review paper, we first discuss various spheroid formation phases. Then, the conventional spheroid formation techniques such as bioreactor flasks, liquid overlay and hanging droplet technique are explained. Next, a particular topic of the hydrogel in spheroid formation and culture is explored. This topic has received less attention in the literature. Hydrogels entail some advantages to the spheroid formation and culture such as size uniformity, the formation of porous spheroids or hetero-spheroids as well as chemosensitivity and invasion assays and protecting from shear stress. Finally, microfluidic methods for spheroid formation and culture are briefly reviewed.

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Spectroscopic imaging system for high-throughput viability assessment of ovarian spheroids or microdissected tumor tissues (MDTs) in a microfluidic chip

Cited by 3 publications

References 6 publications

Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture

Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture

Simulation-assisted design of microfluidic sample traps for optimal trapping and culture of non-adherent single cells, tissues, and spheroids

Inventions and Innovations in Preclinical Platforms for Cancer Research

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