Spectroscopic interaction study of human serum albumin and human hemoglobin with Mersilea quadrifolia leaves extract mediated silver nanoparticles having antibacterial and anticancer activity

Maji, Anukul; Beg, M. M. Sufyan; Mandal, Amit Kumar; Das, Somnath; Jha, Pradeep K.; Kumar, Anoop; Sarwar, Shamila; Hossain, Maidul; Chakrabarti, Pinak

doi:10.1016/j.molstruc.2017.04.005

Cited by 35 publications

(10 citation statements)

References 47 publications

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“…Different cells were employed to reveal the in vitro cytotoxic effect of ZVFe NPs. SH-SY5Y and WBCs were incubated with different concentrations (1,10,20,30,50, 100 µg/mL) of ZVFe NPs for 24 hours. Cell viability was then assessed by MTT assay.…”

Section: Mtt Assaymentioning

confidence: 99%

“…Therefore, it is vital to explore in advance the detailed interaction mechanism between NPs and biological systems such as cell and proteins before using them as medical agents. For example, Maji et al 20 showed that plant extract-mediated silver NPs provide potential protein binding and antibacterial and anticancer activities. Karthika et al 21 reported the fabrication of silver and gold NPs by plant extract and investigated their DNA/protein interactions, anticancer and antimicrobial activities.…”

Section: Introductionmentioning

confidence: 99%

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Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles

Anbouhi¹,

Esfidvajani²,

Nemati³

et al. 2018

IJN

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BackgroundNanoparticles (NPs) have been emerging as potential players in modern medicine with clinical applications ranging from therapeutic purposes to antimicrobial agents. However, before applications in medical agents, some in vitro studies should be done to explore their biological responses.AimIn this study, protein binding, anticancer and antibacterial activates of zero valent iron (ZVFe) were explored.Materials and methodsZVFe nanoparticles were synthesized and fully characterized by X-ray diffraction, field-emission scanning electron microscope, and dynamic light scattering analyses. Afterward, the interaction of ZVFe NPs with human serum albumin (HSA) was examined using a range of techniques including intrinsic fluorescence, circular dichroism, and UV–visible spectroscopic methods. Molecular docking study was run to determine the kind of interaction between ZVFe NPs and HSA. The anticancer influence of ZVFe NPs on SH-SY5Y was examined by MTT and flow cytometry analysis, whereas human white blood cells were used as the control cell. Also, the antibacterial effect of ZVFe NPs was examined on Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), and Staphylococcus aureus (ATCC 25923).ResultsX-ray diffraction, transmission electron microscope, and dynamic light scattering analyses verified the synthesis of ZVFe NPs in a nanosized diameter. Fluorescence spectroscopy analysis showed that ZVFe NPs spontaneously formed a complex with HSA through hydrogen bonds and van der Waals interactions. Also, circular dichroism spectroscopy study revealed that ZVFe NPs did not change the secondary structure of HSA. Moreover, UV–visible data presented that melting temperature (Tm) of HSA in the absence and presence of ZVFe NPs was almost identical. Molecular dynamic study also showed that ZVFe NP came into contact with polar residues on the surface of HSA molecule. Cellular assays showed that ZVFe NPs can induce cell mortality in a dose-dependent manner against SH-SY5Y cells, whereas these NPs did not trigger significant cell mortality against normal white bloods in the concentration range studied (1–100 µg/mL). Antibacterial assays showed a noteworthy inhibition on both bacterial strains.ConclusionIn conclusion, it was revealed that ZVFe NPs did not induce a substantial influence on the structure of protein and cytotoxicity against normal cell, whereas they derived significant anticancer and antibacterial effects.

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Section: Mtt Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles

Anbouhi¹,

Esfidvajani²,

Nemati³

et al. 2018

IJN

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“…Hence there is an urgent need for a commercially viable, economical, rapid, and environmentally friendly route for the detection of Al(III). The optical, chemical, mechanical and electrochemical properties of noble metal nanoparticles make them an excellent resource for varied applications in diverse fields - anticancer 20 , cosmetics 21 , coating 22 , and biocatalysis 3 , 4 . Recently, colorimetric assays and nanoprobes such as paper-based analytical device (PAD), quantum-dots-based photoelectrochemical immune assays, magnetic bead-based reverse colorimetric sensors, MnO 2 nanoflakes with enzyme cascade amplification for colorimetric immunoassay, Fenton reaction-based colorimetric immunoassay for sensitive detection of brevetoxin B have been synthesized for the colorimetric detection of metal ions in aqueous system 23 – 28 .…”

Section: Introductionmentioning

confidence: 99%

Mentha-Stabilized Silver Nanoparticles for High-Performance Colorimetric Detection of Al(III) in Aqueous Systems

Sharma

Dhillon

Kumar

2018

Sci Rep

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The present paper reports a facile and selective colorimetric method for the detection of potential environmental and health hazardous metal ions using green synthesized silver nanoparticles (AgNPs). Here the organic functional groups present in the plant extract (Mentha arvensis) are used as reductants and stabilizers in the synthesis of AgNPs. They also provide a suitable binding site to the (Al(III)) analyte in the detection mechanism. The leaf extract of Mentha arvensis was used to synthesize AgNPs at room-temperature and at 80 °C. The AgNPs synthesized at 80 °C exhibit excellent selective colorimetric detection of Al(III). The as-synthesized AgNPs have been characterized, and the synthesis, stabilization of NPs and detection mechanism has also been illustrated by using UV-vis, XPS, FTIR, TEM, EDX, SEM, AAS, and TGA analytical tools and techniques. The selectivity of detection probe was supported by the reaction between probe and metal ions followed first-order kinetics having the highest value of the regression coefficient (R2 = 0.99) for Al(III) and the analysis of thermodynamic parameters. The prepared sensor showed a lower limit of detection (LOD) of 1 nM (S/N = 3.2) in real water samples. The proposed method can be successfully utilized for the detection of Al(III) from both drinking and real water samples at the nanomolar level.

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“…This result suggest that there is development of MGNs–HSA conjugates and that the HSA protein contributes some negative charge to the MGNs surfaces. This MGNs–HSA conjugate formation also offers dispersion durability to MGNs with the least aggregation, caused by efficient adsorption of HSA onto external MGNs surfaces …”

Section: Resultsmentioning

confidence: 95%

Binding interaction study on human serum albumin with bactericidal gold nanoparticles synthesized from a leaf extract of Musa balbisiana: a multispectroscopic approach

et al. 2019

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This study describes the eco-friendly, low-cost and room-temperature synthesis of gold nanoparticles from Musa balbisiana leaf extract, which acts as both reducing and stabilizing agent, and characterized by ultraviolet−visible (UV-vis) light spectroscopy, fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FE-SEM), analytical transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDAX) and dynamic light scattering (DLS) instruments. These nanoparticles showed an average diameter of 33.83 ± 3.39 nm, which was confirmed from the size distribution histogram. The bactericidal activity of these nanoparticles was confirmed using bacteria Escherichia coli and Staphylococcus aureus at 1 and 2 nM minimum inhibitory concentrations, respectively. The interaction between nanoparticles and human serum albumin (HSA) was investigated, as this plays significant roles in biological systems. The nature of interaction, binding parameters and structural variation of HSA in the presence of these nanoparticles have been evaluated using several useful spectroscopic approaches such as UV-vis, FTIR, time-resolved and steady-state fluorescence, and circular dichroism in addition to the measurement of zeta potential.This interaction study revealed that static quenching occurs in this process with minimal alteration in the secondary structure, but the native structure of HSA remained unaltered. The binding constant and thermodynamic parameters of this interaction process were also evaluated.

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Spectroscopic interaction study of human serum albumin and human hemoglobin with Mersilea quadrifolia leaves extract mediated silver nanoparticles having antibacterial and anticancer activity

Cited by 35 publications

References 47 publications

Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles

Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles

Mentha-Stabilized Silver Nanoparticles for High-Performance Colorimetric Detection of Al(III) in Aqueous Systems

Binding interaction study on human serum albumin with bactericidal gold nanoparticles synthesized from a leaf extract of Musa balbisiana: a multispectroscopic approach

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