Spectroscopic studies on the interaction of bovine serum albumin with Ginkgol C15:1 from Ginkgo biloba L.

Fang, Yangyang; Xiaoming, Yang; Li, Yueying; Feng, Chunlai

doi:10.1016/j.jlumin.2015.02.050

Cited by 14 publications

(7 citation statements)

References 32 publications

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“…Dulbecco's modified Eagle's medium (DMEM), RPMI-1640 medium, fetal bovine serum (FBS) and trypsin-EDTA solution were bought from Shanghai ExCell Biology, Inc. (Shanghai, China). Ginkgol C17:1 (high-performance liquid chromatography purity >96.5%) was obtained from the Laboratory of Food and Biological Engineering School, Jiangsu University (23,25,26). Skimmed milk was purchased from Bright Dairy & Food Co., Ltd. (Harbin, China).…”

Section: Methodsmentioning

confidence: 99%

Differential effects of ginkgol C17:1 on cisplatin‑induced cytotoxicity: Protecting human normal L02 hepatocytes versus sensitizing human hepatoma HepG2 cells

Zhang

Xiaoming

et al. 2019

Oncol Lett

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Liver cancer is a major healthcare problem and one of the leading causes of cancer-associated mortality in the world. To date, chemotherapy remains a common method for treating cancer and cisplatin is one of the most widely used chemotherapeutics. However, owing to drug resistance and side effects, it is imperative to identify a novel approach to improve the anticancer effect of cisplatin. Auxiliary chemotherapy drugs with minor toxicity to normal cells may represent a novel strategy for cancer therapy. Previous studies have indicated that ginkgol C17:1 exhibits anticancer effects in liver cancer cells in vitro and in vivo . The antitumor activity of ginkgol C17:1 has been reported in combination with cisplatin in human liver cancer cells. Owing to the route of systemic administration, liver cancer cells and normal hepatocytes were exposed to chemotherapeutics and auxiliary chemotherapy drugs. However, the effects of ginkgol C17:1 in normal hepatocytes remain unclear. In the present study, the biological effects of ginkgol C17:1 alone and as co-treatment with cisplatin were compared in human hepatoma cells and normal hepatocytes. Consistently, the results confirmed that in human hepatoma HepG2 cells, ginkgol C17:1 or cisplatin alone induced autophagy and apoptosis. The co-treatment increased cisplatin-induced apoptosis and inhibited cisplatin-induced autophagy. In comparison, the treatments in human normal L02 hepatocytes indicated that ginkgol C17:1 alone induced autophagy, whereas cisplatin alone induced apoptosis. The co-treatment inhibited cisplatin-induced apoptosis, but enhanced autophagy in L02 cells. Further investigation revealed that the AMP-activated protein kinase/serine/threonine protein kinase ULK1 and phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathways were involved in the underlying regulatory mechanisms. Taken together, the results of the present study provide the first evidence that ginkgol C17:1 protects normal hepatocytes against cisplatin-induced cytotoxicity while potentiating the anticancer effect of cisplatin chemotherapy. The differential effects on normal and cancer cells suggest that ginkgol C17:1 is a promising candidate for auxiliary chemotherapy.

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Section: Methodsmentioning

confidence: 99%

Differential effects of ginkgol C17:1 on cisplatin‑induced cytotoxicity: Protecting human normal L02 hepatocytes versus sensitizing human hepatoma HepG2 cells

Zhang

Xiaoming

et al. 2019

Oncol Lett

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“…Rabbit anti-EGFR (IM001-0377), rabbit anti-Akt (IM001-0359) and rabbit anti-p-Akt1/2/3 (Tyr315/316/312, IM001-0270) polyclonal antibodies were purchased from ExCell Biology Co., (Shanghai, China). Ginkgol C17:1 (HPLC purity>96.5%) was kindly provided from Dr. Yang at the Laboratory of Food and Biological Engineering School of Jiangsu University [ 12 - 13 ] .…”

Section: Methodsmentioning

confidence: 99%

Ginkgol C17:1 inhibits tumor growth by blunting the EGF-PI3K/Akt signaling pathway

Li¹,

Liu²,

Xiaoming³

et al. 2017

J Biomed Res

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“…Ginkgols (Figure 2a), also known as 3‐alkylphenols, are organic phytochemicals in GB. The alkyl side chain ranges from 13 to 17 carbons, with 0–2 double bonds (Fang et al, 2015). Ginkgols consist of six homologs with alkyl substituents C13:0, C17:2‐Δ 9, 12 , C15:1‐Δ 8 , C15:0, C17:1‐Δ 12 , and C17:1‐Δ 10 , as shown in Figure 2a (Li et al, 2022).…”

Section: Ginkgolsmentioning

confidence: 99%

“…Ginkgols consist of six homologs with alkyl substituents C13:0, C17:2‐Δ 9, 12 , C15:1‐Δ 8 , C15:0, C17:1‐Δ 12 , and C17:1‐Δ 10 , as shown in Figure 2a (Li et al, 2022). The ginkgols exert pharmacological effects, including anti‐oxidant, anti‐apoptotic, and anti‐bacterial effects (Fang et al, 2015; Li, Liu, Liu, et al, 2017). Furthermore, research by Yang et al (2014) proved that ginkgols also demonstrated tremendous anticancer impacts and thermostability.…”

Section: Ginkgolsmentioning

confidence: 99%

“…Ginkgols in GB (Figure 2a), active compounds, have been linked to several pharmacological properties in recent years, including anti‐oxidant, anti‐apoptotic, anti‐bacterial activities (Fang et al, 2015; Li, Liu, Liu, et al, 2017; Tobinaga et al, 2012). Furthermore, in studies by Yang et al (2014) and Li et al (2022), the authors showed that ginkgols had tremendous anticancer impacts and thermostability, as ginkgols (C13:0, C15:1, C17:1‐Δ 12 , and C17:1‐Δ 10 ) were separated, and their antiproliferation impacts on cancer cells were examined.…”

Section: Introductionmentioning

confidence: 99%

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Ginkgols and bilobols in Ginkgo biloba L. A review of their extraction and bioactivities

Boateng

2023

Phytotherapy Research

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Ginkgo biloba (GB) has enormous bioactives with anti‐bacterial, anti‐oxidant, anti‐cancer, and immune‐stimulating properties, with global sales exceeding $10 billion. The terpene trilactones (ginkgolides A, B, and C) and flavonoids (mostly quercetin, isorhamnetin, and kaempferol) have received the most significant focus in GB research to date, whereas other bioactive compounds such as ginkgols and bilobols with various bioactivities such as anti‐viral, anti‐oxidant, and anti‐tumor actions have received less attention. Therefore, for the first time, this review focused on GB ginkgols, bilobols extraction, and bioactivities. This review showed that petroleum ether and acetone extraction had successfully extracted ginkgols and bilobols. Furthermore, bioactivities such as anti‐tumor activity and so on have been demonstrated for ginkgols, and bilobols, providing theoretical justification for ginkgols and bilobol as raw material for nutraceuticals, functional foods, pharmaceuticals, and cosmeceuticals. Future research could look into other biological applications (such as anti‐oxidant, antitoxins, anti‐radiation, anti‐microbial, and antiparasite) and their applications in the pharmaceutical, cosmetic, and nutraceutical industries. Besides, the primary research should be on developing green and effective methods for preparing ginkgols and bilobols and fully utilizing their pharmacological activity. This will also provide a new avenue for efficiently utilizing these bioactive compounds.

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Spectroscopic studies on the interaction of bovine serum albumin with Ginkgol C15:1 from Ginkgo biloba L.

Cited by 14 publications

References 32 publications

Differential effects of ginkgol C17:1 on cisplatin‑induced cytotoxicity: Protecting human normal L02 hepatocytes versus sensitizing human hepatoma HepG2 cells

Differential effects of ginkgol C17:1 on cisplatin‑induced cytotoxicity: Protecting human normal L02 hepatocytes versus sensitizing human hepatoma HepG2 cells

Ginkgol C17:1 inhibits tumor growth by blunting the EGF-PI3K/Akt signaling pathway

Ginkgols and bilobols in Ginkgo biloba L. A review of their extraction and bioactivities

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