Spectroscopy Analysis of Silver Nanoparticles Synthesized from citrus Limon Leaf Extract and Their Biological Effects

Abstract

Due to its efficiency, eco-friendliness, non-toxicity, and cost-effectiveness, the utilisation of plant extracts in the manufacture of silver nanoparticles has garnered attention. The purpose of this study is to determine the feasibility of generating silver nanoparticles from an aqueous extract of Citrus limon leaves and their size and composition was characterized utilizing UV visible, FTIR, SEM, EDX, and Zeta seizer. Additionally, the antioxidant efficacy of Ag NPs was tested using the DPPH radical scavenging method, and their antibacterial activity was verified in vitro using the Agar well diffusion technique. Silver nanoparticles were successfully synthesized and exhibit surface plasmon resonance with an absorption maximum of 475.2 nm and the stabilization of the ions Ag to nanometric spherical negative charge particles, as verified by FTIR, MEB, EDX, and Zeta potential values. Furthermore, the silver nanoparticles created displayed a substantial bactericidal effect against the three types of bacteria (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) and an antifungal activity against (Candida albicans) as well as reasonable antioxidant properties.