Spectroscopy, Photoacoustic

Harren, Frans J. M.; Reuß, J.

doi:10.1002/3527600434.eap466

Cited by 16 publications

(17 citation statements)

References 28 publications

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“…All samples were immediately frozen in liquid nitrogen and stored at 280°C for later molecular analyses. The laser-based photoacoustic detection of ethylene was carried out on four biological replicates using a previously described experimental device (Harren et al, 1990a(Harren et al, , 1990bHarren and Reuss, 1997) as reported by Dal Cin et al (2005Cin et al ( , 2007. A suitable number of fruitlets, varying from 10 to 15 (according to their size), were assessed for each biological replicate.…”

Section: Plant Materials and Measurementsmentioning

confidence: 99%

Roles of Ethylene Production and Ethylene Receptor Expression in Regulating Apple Fruitlet Abscission

et al. 2015

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Apple (Malus 3 domestica) is increasingly being considered an interesting model species for studying early fruit development, during which an extremely relevant phenomenon, fruitlet abscission, may occur as a response to both endogenous and/or exogenous cues. Several studies were carried out shedding light on the main physiological and molecular events leading to the selective release of lateral fruitlets within a corymb, either occurring naturally or as a result of a thinning treatment. Several studies pointed out a clear association between a rise of ethylene biosynthetic levels in the fruitlet and its tendency to abscise. A direct mechanistic link, however, has not yet been established between this gaseous hormone and the generation of the abscission signal within the fruit. In this work, the role of ethylene during the very early stages of abscission induction was investigated in fruitlet populations with different abscission potentials due either to the natural correlative inhibitions determining the so-called physiological fruit drop or to a well-tested thinning treatment performed with the cytokinin benzyladenine. A crucial role was ascribed to the ratio between the ethylene produced by the cortex and the expression of ethylene receptor genes in the seed. This ratio would determine the final probability to abscise. A working model has been proposed consistent with the differential distribution of four receptor transcripts within the seed, which resembles a spatially progressive cell-specific immune-like mechanism evolved by apple to protect the embryo from harmful ethylene.

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Section: Plant Materials and Measurementsmentioning

confidence: 99%

Roles of Ethylene Production and Ethylene Receptor Expression in Regulating Apple Fruitlet Abscission

et al. 2015

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“…Ethylene, a well-known indicator for lipid peroxidation (Kneepkens et al 1994) was measured using laser-based photoacoustic detection, as described in Harren and co-workers (Harren et al 1999, Harren andReuss 1997). Briefly, the infrared laser is tuned to a wavelength where the gas under investigation has a high absorption coefficient.…”

Section: Instrumentationmentioning

confidence: 99%

The suitability of Tedlar bags for breath sampling in medical diagnostic research

Steeghs

Cristescu²,

Harren³

2006

Physiol. Meas.

112

133

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Tedlar bags are tested for their suitability for breath sampling for medical diagnostic purposes. Proton-transfer reaction-mass spectrometry was used to monitor the changes in composition of various mixtures contained in custommade black-layered Tedlar bags. Characteristic ions at m/z 88 and 95 amu reflect considerable pollution from the bag material. The pollutant found on m/z 88 amu is most probably N,N-dimethylacetamide, a latent solvent used in the production of Tedlar film. Gas composition losses during filling were found to range from 5 to 47%, depending on the compound. Once stored, the half-lives of methanol, acetaldehyde, acetone, isoprene, benzene, toluene and styrene were estimated between 5 and 13 days. Losses from breath samples (52 h after filling) were found to be less than 10%. No observable decrease was found for ethylene over 3 days, using laser-based photoacoustic detection. For the use of Tedlar bags, a standardized protocol is advised, where the time point of analysis is fixed for all samples and should be kept as close as possible to the time of sampling.

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“…Subsequently, many experimental and theoretical works have been reported in the literature, proving the applicability of the method not only in spectroscopy, but also in various fields of physics, chemistry, biology, medicine, and engineering. The potential of laser photoacoustic spectroscopy has been discussed in several review articles (Patel & Tam, 1981;West, 1983;Hess, 1983;Tam, 1986;Sigrist, 1986Sigrist, , 2003Meyer & Sigrist, 1990;Harren & Reuss, 1997;Harren et al, 2000;Miklos et al, 2001;Schmid, 2006) and books (Pao, 1977;Rosencwaig, 1980;Zharov & Letokhov, 1986;Hess, 1989;Mandelis, 1992Mandelis, , 1994Bicanic, 1992;Gusev & Karabutov, 1993;Sigrist, 1994;Mandelis & Hess, 1997).…”

Section: Historical Remarksmentioning

confidence: 99%