Spectrum-effect relationship between serum HPLC fingerprints and activation of blood circulation and removal of blood stasis by Chuanxiong

Abstract


 
 
 
 Purpose: To study the active ingredients of Chuanxiong (CX) in promoting blood circulation and removing blood stasis.
 Methods: Blood-activating effects and serum HPLC fingerprints of CX extracts from different parts of China were studied and the spectrum-effect relationship between them was established by grey correlation analysis.
 Results: After treatment with CX extracted using different solvents, hemorheology indices were lower than those in the model group, and the effect of dichloromethane was better than that of other solvents (p < 0.05 or p < 0.01). There were 6 common peaks by fingerprint analysis. Peaks 1 - 5 were identified as senkyunolide I, senkyunolide H, senkyunolide A, coniferyl ferulate and ligustilide, respectively.
 Conclusion: Analysis of the spectrum–effect relationship indicates the contribution of the five components to the blood-activating effect of CX. The findings lend some scientific justification for using CX to remove blood stasis, and selection of quality evaluation indices for CX.