Speed vernalization to accelerate generation advance in winter cereal crops

Cha, Jin-Kyung; O'Connor, Kathryn; Alahmad, Samir; Lee, Jonghee; Dinglasan, Eric; Park, Hyeonjin; Lee, So-Myeong; Hirsz, Dominique; Kwon, Soon Jae; Kwon, Young-In; Kim, Kyeong-Min; Ko, Jong-Min; Hickey, Lee T.; Shin, Dongjin; Dixon, Laura E.

doi:10.1016/j.molp.2022.06.012

Cited by 38 publications

(34 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…22 h settings reduced flowering times numerically and/or statistically in all the vernalization duration treatments. In comparison with Cha et al (2022), where 2 weeks of vernalization was used on uncovered seeds, and quick generation times were observed, but 12% of lines did not reach reproductive maturity, all of our tested lines reached flowering effectively.…”

Section: Discussionmentioning

confidence: 67%

“…Though this is a slight reduction (2-3 days) in generation time, it is still not sufficient for breeding purposes. Further, a study by Cha et al (2022) showed significant success in decreasing generation time in winter wheat by combining "Speed Vernalization", which involves an increased day/night cycle during cold treatment, with long day growing conditions post-vernalization. However, only 45 of 51 tested wheat varieties showed success with this method, with six cultivars not reaching flowering due to the modified vernalization.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Reducing the generation time in winter wheat cultivars using speed breeding

et al. 2023

View full text Add to dashboard Cite

Reducing generation time is critical to achieving the goals of genetic gain in important crops like wheat (Triticum aestivum). Speed breeding (SB) has been shown to considerably reduce generation times in crop plants. Unlike spring wheat cultivars, winter wheat varieties require typically 6-9 weeks of cold treatment, called vernalization, for flowering which extends the generation time for the development of improved winter wheat cultivars. Here, we optimized the SB method using a set of 48 diverse soft red winter wheat (SRWW) cultivars by testing vernalization duration, light and temperature requirements, and the viability of seeds harvested after different durations post-anthesis under extended daylight conditions. We have found that using a 22-h setting (22 h day/2 h night, 25˚C/22˚C) in high-density 50-cell trays results in rapid generation advancement. We used genotypic data for a panel of soft red winter wheat varieties from the regional programs to determine the impact of photoperiod and vernalization alleles on the efficiency of the SB approach. Using a set of 48 SRWW cultivars and germplasm from Maryland and four other public breeding programs, we establish that this protocol can allow for the advancement of four generations per year in controlled conditions for winter wheat varieties, experimental lines, or emerging cultivars. Our work shows the potential to reduce generation time Abbreviations: DH, doubled haploids; DPF, days post flowering; HID, high intensity discharge; SB, speed breeding; SSD, single seed descent.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

show abstract

Section: Discussionmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Reducing the generation time in winter wheat cultivars using speed breeding

et al. 2023

View full text Add to dashboard Cite

show abstract

“…However, only one winter wheat variety was assessed in that study. By exposing developing kernels at soil surface at 10°C to an extended photoperiod of 22 , Cha et al (2022) recently reported the winter wheat and barley could be processed up to five generations per annum. Clearly, neither of these methods could match the protocol described in our study, due likely to the fact that less times are required to vernalize seedlings from immature embryos (Dubert et al, 1993; Sharma & Gill, 1982; Sharma & Masci, 1987).…”

Section: Discussionmentioning

confidence: 99%

Shortening generation times for winter cereals by vernalizing seedlings from young embryos at 10 degree Celsius

et al. 2023

View full text Add to dashboard Cite

Methods allowing quick generation cycles are available for various crop species but are limited to spring genotypes. However, winter types predominate in many crops including wheat and barley. Different from spring ones, winter genotypes need to be treated in low temperature for extended periods to accelerate flowering. Combined with known factors that reduce generation cycles for spring wheat and barley, the generation cycle effects of vernalizing seedlings from young embryos of winter types at 10°C of several crop species were studied. Here, we described how to obtain up to seven generations of winter barley, six generations for winter wheat or five generations for both winter oat and triticale genotypes per annum. This procedure should find wide applications in breeding and other biological studies.

show abstract

“…Increased availability of the genome and transcriptome sequencing, along with the improvements in computational biology, may reveal new molecular mechanisms involved in the vernalization pathway. VanGessel et al (2022) recently demonstrated transcriptional signatures of inflorescent development in the tetraploid wheat variety Kronos. The gene expression atlas of the floral meristem based on single nucleus RNA-seq data was developed latterly in barley (Neumann et al, 2022).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Contemplation on wheat vernalization

Milec

Strejčková²,

Šafář³

2023

Front. Plant Sci.

View full text Add to dashboard Cite

Vernalization is a period of low non-freezing temperatures, which provides the competence to flower. This mechanism ensures that plants sown before winter develop reproductive organs in more favourable conditions during spring. Such an evolutionary mechanism has evolved in both monocot and eudicot plants. Studies in monocots, represented by temperate cereals like wheat and barley, have identified and proposed the VERNALIZATION1 (VRN1) gene as a key player in the vernalization response. VRN1 belongs to MADS-box transcription factors and is expressed in the leaves and the apical meristem, where it subsequently promotes flowering. Despite substantial research advancement in the last two decades, there are still gaps in our understanding of the vernalization mechanism. Here we summarise the present knowledge of wheat vernalization. We discuss VRN1 allelic variation, review vernalization models, talk VRN1 copy number variation and devernalization phenomenon. Finally, we suggest possible future directions of the vernalization research in wheat.

show abstract

Speed vernalization to accelerate generation advance in winter cereal crops

Cited by 38 publications

References 24 publications

Reducing the generation time in winter wheat cultivars using speed breeding

Reducing the generation time in winter wheat cultivars using speed breeding

Shortening generation times for winter cereals by vernalizing seedlings from young embryos at 10 degree Celsius

Contemplation on wheat vernalization

Contact Info

Product

Resources

About