Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques

Larson, Kjersten; DeJonge, Christopher J.; Barnes, Annette M.; Jost, Lorna K.; Evenson, Donald P.

doi:10.1093/humrep/15.8.1717

Cited by 433 publications

(277 citation statements)

References 41 publications

Supporting

Mentioning

233

Contrasting

Unclassified

Order By: Relevance

“…Although the threshold, for sperm DFI in infertile men is established [5,8,15,44] the role of sperm factor in iRPL following spontaneous conception has not been extensively evaluated [3,20], and DFI cut -off value has not yet been established. This study has tried to establish a threshold value of sperm DFI in cases with iRPL following spontaneous conception for the first time in India and is amongst the few studies in the world [28,39]. Studies have suggested that pregnancy is unlikely to occur when sperm nuclear DFI is above a certain threshold value [28,39].…”

Section: Discussionmentioning

confidence: 99%

“…This study has tried to establish a threshold value of sperm DFI in cases with iRPL following spontaneous conception for the first time in India and is amongst the few studies in the world [28,39]. Studies have suggested that pregnancy is unlikely to occur when sperm nuclear DFI is above a certain threshold value [28,39]. Using sperm chromatin dispersion test (SCD), Bellver et al [3] observed that sperm DNA fragmentation was higher in the recurrent spontaneous abortion group compared with the sperm donor group.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Kumar

Deka

Singh

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose Standard semen parameters are poor predictors of fertility potential. To date, apart from, paternal karyotyping sperm factors are not evaluated in recurrent pregnancy loss (RPL), only recent studies have emphasized the role of sperm factors in early embryonic development as sperm transcribes genes critical for early embryonic development. Sperm DNA integrity is useful diagnostic and prognostic marker and has clinical implications in idiopathic recurrent pregnancy loss (iRPL) following spontaneous conception. The aim of this study was to assess DNA integrity in cases experiencing iRPL following spontaneous conception. Methods Semen samples from 45 patients and 20 controls were analyzed as per WHO 1999 guidelines and sperm chromatin structure assay (SCSA) was used to measure DNA fragmentation index (DFI). Results By applying receiver operating curve (ROC) analysis, sperm DFI of approximately 26 % was found in male partner of couples experiencing iRPL. Conclusions Our data indicate that sperm from men with a history of iRPL have a higher percentage of DNA damage as compared to control group, and this can explain pregnancy loss in these patients. Men with higher DFI are infertile whereas men with lower DFI (26 %) are able to conceive but experience recurrent pregnancy loss. Thus it is important to evaluate sperm DFI in couples experiencing iRPL to understand exact aetiology of RPL and determine prognosis and management.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Kumar

Deka

Singh

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…The need to reevaluate old test scores is best illustrated by how a reevaluation of the use of the SCSA DNA fragmentation index (DFI) scores has dramatically changed how the test results are used to direct patients to appropriate ART [16,17]. The SCSA is the only DNA integrity test with substantial scientific literature supporting the credibility of this test for use in diagnosing male infertility, and up until 2007, patients whose sperm had a DNA fragmentation index (DFI)≥30 were told that their sperm had minimal to no chance for successful ART attempts at pregnancy [22,31,32]. However, as the number of clinics using ICSI in treating their infertile couples, this DFI cut-off began to be questioned as many groups using sperm with DFIs of 30 or higher were having excellent live birth outcome rates [23,43,60].…”

Section: Discussionmentioning

confidence: 99%

Evaluating a novel panel of sperm function tests for utility in predicting intracytoplasmic sperm injection (ICSI) outcome

Brown

Merryman²,

Rivnay

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The objective of this study was to evaluate a panel of three sperm function tests; tests known to assess different aspects of sperm functionality and genomic integrity, the: 1) Sperm DNA Accelerated Decondensation (SDAD TM ) Test, 2) Sperm DNA Decondensation (SDD TM ) Test, and 3) Sperm Penetration Assay (SPA), determining if positive and negative test scores correlated with failed and successful ICSI outcomes, respectfully. Methods A prospective, double blinded, cohort study was performed. One study sample (ejaculated semen) was collected by each of the 60 male partners of the 60 couples enrolled in the study; males whose female partners were found to have no major female factor issues. The sperm from each male was analyzed in the SPA, and SDAD and SDD Tests, and used for ICSI (1 ICSI cycle per couple). Results The ICSI cycle pregnancy rate for this study was 50 %, with a delivery rate040 % (n060 ICSI cycles). The SPA and SDD Test scores did not significantly predict ICSI outcome when used as stand-alone tests (p>>0.05). However, when the SPA and SDD Test scores were used together, ICSI outcomes for a subgroup of 10 (16.7 %) males, were significantly predicted (p00.03), with 1 live birth, and 9 negatives where the transferred embryos did not implant. In total, 38.4 % of the couples in this study were found to have a very poor chance for a successful ICSI cycle. Conclusion SDAD Test scores alone, and SPA and SDD Test scores used together, significantly predicted failed ICSI outcomes. This indicates that the scores obtained when analyzing patients' sperm using a panel of sperm function tests; specifically, the SPA, and SDAD and SDD Tests, can be used to identify infertile couples who should not be directed to ICSI.

show abstract

“…It is well recognized that the percentage of progressively motile sperm is associated with pregnancy rates 16,21,22 and great care should be taken to ensure accurate measurements.…”

Section: Assessment Of Motilitymentioning

confidence: 99%

Semen analysis and sperm function testing

Franken¹,

Oehninger²

2011

Asian J Androl

View full text Add to dashboard Cite

Despite controversy regarding the clinical value of semen analysis, male fertility investigation still relies on a standardized analysis of the semen parameters. This is especially true for infertility clinics in both developing and developed countries. Other optional tests or sophisticated technologies have not been widely applied. The current review addresses important changes in the analysis of semen as described in the new World Health Organization (WHO) manual for semen analysis. The most important change in the manual is the use of evidence-based publications as references to determine cutoff values for normality. Apart from the above mentioned changes, the initial evaluation and handling methods remain, in most instances, the same as in previous editions. Furthermore, the review evaluates the importance of quality control in andrology with emphasis on the evaluation of sperm morphology. WHO sperm morphology training programmes for Sub-Saharan countries were initiated at Tygerberg Hospital in 1995. The external quality control programme has ensured that the majority of participants have maintained their morphological reading skills acquired during initial training. This review reports on current sperm functional tests, such as the induced acrosome reaction, and sperm-zona pellucida binding assays, as well as the impact of sperm quality in terms of DNA integrity, and the relationship of sperm function tests to sperm morphology.

show abstract

Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques

Cited by 433 publications

References 41 publications

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Evaluating a novel panel of sperm function tests for utility in predicting intracytoplasmic sperm injection (ICSI) outcome

Semen analysis and sperm function testing

Contact Info

Product

Resources

About