Sperm content of postacrosomal WW binding protein is related to fertilization outcomes in patients undergoing assisted reproductive technology

Aarabi, Mahmoud; Bałakier, Hanna; Bashar, Siamak; Moskovtsev, Sergey I.; Šutovský, Peter; Librach, Clifford L.; Oko, Richard

doi:10.1016/j.fertnstert.2014.05.003

Cited by 56 publications

(63 citation statements)

References 37 publications

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“…These recent findings from Aarabi et al (47,49) have led to significant debate regarding the essential role of these 2 proteins (i.e., PLCz and PAWP) as oocyte activation factors (50)(51)(52). However, the true extent of the functional role of PAWP, and its precise relevance in the oocyte activation process, should be treated with caution, until further evidence is obtained, because the results of a recent study directly conflict with those of Aarabi et al (47), in that they failed to demonstrate calcium oscillations in mouse oocytes in response to the injection of recombinant PAWP (28).…”

Section: Discussionmentioning

confidence: 71%

“…Despite this evidence, recent studies suggest that a postacrosomal sperm protein found in the perinuclear theca, and known as PAWP (postacrosomal WW binding protein), elicits calcium oscillations similar to those seen during fertilization in mammalian (46,47) and nonmammalian oocytes (48). Furthermore, a significant correlation between PAWP levels in human sperm, and fertilization rates after ICSI, has been reported (49).…”

Section: Discussionmentioning

confidence: 99%

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Total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta are significantly correlated with fertilization rates after intracytoplasmic sperm injection

Yelumalai

Yeste

Jones

et al. 2015

Fertility and Sterility

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta are significantly correlated with fertilization rates after intracytoplasmic sperm injection

Yelumalai

Yeste

Jones

et al. 2015

Fertility and Sterility

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“…Polyclonal rabbit serum was raised against human recombinant (r) WBP2NL protein that was produced from WBP2NLcDNA expression vector (pET28a-N-HisTag) within E. coli host BL21 (DE3). The immune serum was then affinity purified on immobilized His-tag purified rWBP2NL protein and eluted with glycine HCL followed by neutralization with TBS (pH 8) [26].…”

Section: Antibodiesmentioning

confidence: 99%

WBP2 shares a common location in mouse spermatozoa with WBP2NL/PAWP and like its descendent is a candidate mouse oocyte activating factor

Hamilton

Suzuki

Acteau

et al. 2018

Biology of Reproduction

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The sperm-borne oocyte-activating factor (SOAF) resides in the sperm perinuclear theca (PT). A consensus has been reached that SOAF most likely resides in the postacrosomal sheath (PAS), which is the first region of the PT to solubilize upon sperm-oocyte fusion. There are two SOAF candidates under consideration: PLCZ1 and WBP2NL. A mouse gene germline ablation of the latter showed that mice remain fertile with no observable phenotype despite the fact that a competitive inhibitor of WBP2NL, derived from its PPXY motif, blocks oocyte activation when coinjected with WBP2NL or spermatozoa. This suggested that the ortholog of WBP2NL, WBP2, containing the same domain and motifs associated with WBP2NL function, might compensate for its deficiency in oocyte activation. Our objectives were to examine whether WBP2 meets the developmental criteria established for SOAF and whether it has oocyte-activating potential. Immunoblotting detected WBP2 in mice testis and sperm and immunofluorescence localized WBP2 to the PAS and perforatorium of the PT. Immunohistochemistry of the testes revealed that WBP2 reactivity was highest in round spermatids and immunofluorescence detected WBP2 in the cytoplasmic lobe of elongating spermatids and colocalized it with the microtubular manchette during PT assembly. Microinjection of the recombinant forms of WBP2 and WBP2NL into metaphase II mouse oocytes resulted in comparable rates of oocyte activation. This study shows that WBP2 shares a similar testicular developmental pattern and location with WBP2NL and a shared ability to activate the oocyte, supporting its consideration as a mouse SOAF component that can compensate for a WBP2NL.

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“…Absence of SOAF(s) in globozoospermic individuals because of absence of acrosome and PAS-PT (Lawrence et al, 1997;Neri et al, 2014) during acrosome biogenesis in these individuals may be considered as one reason for failed fertilization in globozoospermic men. A number of candidates have been proposed as SOAF including PLCf (Saunders et al, 2002;Swann et al, 2006) (a spermspecific phospholipase C), TR-KIT (a truncated form of the KIT receptor) (Sette et al, 1997), PAWP (post-acrosomal sheath WWdomain-binding protein) (Wu et al, 2007a,b;Aarabi et al, 2014a) and citrate synthase (Harada et al, 2007). Among these factors, PLCf has emerged as the strongest candidate and several studies have shown the correlation between absence or deficiency of PLCf with failed fertilization in globozoospermic men (Heytens et al, 2009;and Aghajanpour et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Expression profile ofPLCζ,PAWP,andTR-KITin association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation

Tavalaee

Nasr-Esfahani

2016

Andrology

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SUMMARYSperm-mediated oocyte activation critically depends upon appropriate expression and assembly of sperm-borne oocyte-activating factors (SOAFs) during spermiogenesis. Several factors have been considered as candidate for SOAFs over the recent years. However, little is known about the expression profile of these candidates and their potential contribution to the clinical outcomes of intra-cytoplasmic sperm injection (ICSI), particularly in globozoospermia. This study investigated the expression profile of PLCf, PAWP, and TR-KIT and clinical outcomes of ICSI in 12 men with total globozoospermia and compared with 12 fertile individuals. Levels of PLCf, PAWP, and TR-KIT mRNA in the spermatozoa of fertile men were significantly higher than the corresponding values of the globozoospermic subjects. Interestingly, at protein level, expressions of these factors in the cases assessed were low in globozoospermic individuals. Fertilization rates following artificial oocyte activation (AOA) in the majority of globozoospermic couples were higher than the expected 30% cut-off value reported for individuals with failed or low fertilization rate. Clinical outcomes of ICSI-AOA were dependent on inter-individual variation in globozoospermic couples. None of the SOAFs assessed could provide a greater prediction value with respect to fertilization rate in globozoospermic couples which underwent ICSI-AOA. High fertilization (56.06%) and pregnancy (41.7%) rates accomplished in this study following ICSI-AOA indicated that expression profiles of PLCf, PAWP, and TR-KIT were low in globozoospermic individuals, and ICSI combined with artificial oocyte activation could mimic physiological calcium changes which occur during fertilization.

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Sperm content of postacrosomal WW binding protein is related to fertilization outcomes in patients undergoing assisted reproductive technology

Cited by 56 publications

References 37 publications

Total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta are significantly correlated with fertilization rates after intracytoplasmic sperm injection

Total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta are significantly correlated with fertilization rates after intracytoplasmic sperm injection

WBP2 shares a common location in mouse spermatozoa with WBP2NL/PAWP and like its descendent is a candidate mouse oocyte activating factor

Expression profile ofPLCζ,PAWP,andTR-KITin association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation

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